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Biomedical subjects

M H Mostafa

Publications and source records attributed to M H Mostafa.

At least 73 records · Page 4Linked to original sources
PubMed1979

Effect of chlorpromazine free radical on tyrosine hydroxylase of bovine adrenal medulla.

The effect of preincubation with chlorpromazine and chlorpromazine free radical on tyrosine hydroxylase activity was studied. Chlorpromazine free radical inhibited the enzyme activity to a greater extent than chlorpromazine. More than a two-fold decrease in the enzyme activity was observed following the preincubation of the enzyme with chlorpromazine free radical. This observation may suggest interaction between chlorpromazine free radical and -SH group located on the active site of the apoenzyme. Another interaction between chlorpromazine free radical and the coenzyme (DMPH4) was confirmed from spectral studies.

Adrenal Medulla↗
PubMed1978

The activities of urinary alpha-esterases in bilharziasis and their possible role in the diagnosis of bilharzial bladder cancer in Egypt.

The activities of serum and urinary alpha-esterases were studied in 49 bilharzial bladder cancer patients, 92 bilharzial patients with other, different, urologic diseases and 22 normal healthy controls. Among the group studied, the bilharzial bladder cancer patients showed the highest level of urinary-alpha-esterases and the lowest ratio of serum/urine activity. These patients showed a significant increase in the activity of urinary alpha-esterases as compared with the normal controls or with the other different bilharzial groups. Although a small but significant increase in serum activity was observed in all the bilharzial groups studied compared with the normal healthy controls, no significant difference was found between the bilharzial bladder cancer patients and the other different bilharzial groups. Biochemical determination of urinary alpha-esterases for bilharzial patients revealed a high degree of accuracy for the diagnosis of bilharzial bladder cancer (95.9%), with low falsely positive (3.26%) and falsely negative (4.08%) results. The activity of urinary alpha-esterases in recommended as a screening test for bilharzial bladder cancer.

Egypt↗
PubMed1978

In vitro studies of the effect of metal ions, EDTA and their mixtures on kynurenine aminotransferase and kynurenine hydrolase.

In the present study use was made of the chelating ability of EDTA and the activating property of some metal ions Ca(II), Mg(II) or Mn(II) to counteract the inhibitory effect of Cu(II), Co(II), Pb(II) or Zn(II) ions on the B6-dependent kynurenine hydrolase and on kynurenine aminotransferase. These may be of help in studying the therapeutic trials in the treatment of metal poisoning. EDTA was able to counteract the inhibitory effect of Cu(II) or Co(II) on kynurenine aminotransferase and partially counteract the inhibitory effect of Cu(II), Co(II) on kynurenine aminotransferase and partially counteract the inhibitory effect of Cu(II), Co(II), Pb(II) or Zn(II) ions on kynurenine hydrolase. The difference in the response of the two B6-dependent enzymes to EDTA is attributed to the difference in the functional groups involved in the active site(s) of the two apoenzymes. Moreover, Mn(II), Ca(II) and Mg(II) ions have the ability to counteract some of the inhibitory effect of these metal ions.

Animals↗
PubMed1978

The effect of environment on tryptophan metabolism "via kynurenine" in oral contraceptives users.

We have studied tryptophan metabolism "via kynurenine" in women who were oral contraceptives users, living in two different egyptian environments: in the city of Alexandria or in a rural area near Tanta and, in comparison, in control groups of women living in the same environments but who were not contraceptives users. In the non contraceptives users of Tanta we have registered a higher elimination of some metabolites (anthranilic acid, kynurenic acid, xanthurenic acid and 3-OH-anthranilic acid) which might be related to the way of life and environment. The pattern of the kynurenine pathway metabolites of the contraceptives users (studied 1, 3 and 12 months after contraceptives administration) shows some differences with respect to the controls, both in the spontaneous elimination and in that after tryptophan load. These differences are especially evident for anthranilic acid glucuronide, kynurenine and kynurenic, anthranilic and xanthurenic acids. Some differences may be observed after 1 month of contraceptives administration. The effect of tryptophan oral load is superimposed on those of environment and contraceptives use; in the spontaneous elimination of tryptophan metabolites an accomodation to the contraceptives administration is evident. The discrepancies between some of our results and those of the literature might be related to the influence of environmental factors and of the way of life.

Aminohippuric Acids↗
PubMed1977

beta-Glucuronidase activity in liver and spleen homogenates of mice experimentally infected with Schistosoma mansoni.

A study on the beta-glucuronidase activity in liver and spleen homogenates of mice infected with an Egyptian strain of Schistosoma mansoni and of non infected control animals was carried out for a follow up period of four months. A decreased enzyme activity was observed in the spleen up to the 40th day after infection. From the 60th day on, the enzyme level in both liver and spleen was found above that in the control. The possible causes for such changes in enzyme activity are discussed.

Animals↗
PubMedDate not supplied

Possible mechanisms of alteration in the capacities of carcinogen metabolizing enzymes during schistosomiasis and their role in bladder cancer induction.

Carcinoma of the urinary bladder is the most common malignancy in many tropical and subtropical countries. There is a well documented association with chronic urinary schistosomal infection, and bladder cancer associated with schistosomiasis is a major cause of morbidity and mortality in the endemic areas. Many factors have been suggested as possible causative agents in schistosome-associated bladder carcinogenesis but theories concerning the possible role of schistosomal infection in altering host metabolism of chemical carcinogens have received most attention. In experimental schistosomiasis there is a common pattern of changes in the activities of several hepatic Phase I and Phase II enzymes. Phase I enzymes show increased activities in the early stages of infection but these activities are reduced to below their preinfection levels in the intermediate and late chronic stages of the disease. The activities of Phase II enzymes are altered in favour of the deconjugation pathways in the later stages of the disease. The possible basic mechanisms that might be involved in such changes during parasitism and their potential role in the induction of bladder neoplasia are discussed.

Animals↗