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Biomedical subjects

M H Panthaki

Publications and source records attributed to M H Panthaki.

12 recordsLinked to original sources

Evaluation of phospholipase activity of Candida albicans and its correlation with pathogenicity in mice.

Sixty-one isolates of Candida albicans were tested for their phospholipase activity and this parameter was correlated with pathogenicity in albino mice. Of the isolates tested, 57 (93%) showed appreciable phospholipase activity and of these, 55 (90%) were pathogenic to mice. A significant correlation was found between phospholipase activity and involvement of kidneys in animal pathogenicity studies. The isolates of C. albicans, that exhibited higher phospholipase activity were found to be pathogenic for mice.

Animals↗

Detection of haemoglobin variants for the diagnosis of beta thalassaemia & other haemoglobinopathies using anion exchange high performance liquid chromatography.

We describe the precise identification, separation and quantitation of several haemoglobin (Hb) variants such as Hbs A2, A, F, S, E, J, H, D-Punjab and Q-India in untransfused patients with beta thalassaemia and other haemoglobinopathies prevalent in the Indian population using whole blood (5 microliters), red cell lysates (2 microliters) or dried blood specimens on filter paper, using a conventional anion exchange high performance liquid chromatography (AX-HPLC) system. Samples were simultaneously studied for the presence of haemoglobin variants by alkaline electrophoresis. The problems in analysing rare variants and separating the closely migrating variants, encountered in the conventional methods could be resolved using the HPLC technique.

Adult↗

Doubting atopy?

Sera of 37 patients attending the allergic clinic and of 10 normal healthy non-allergic controls were collected and in a double blind manner they were tested using phadiatop RIA for differentiation of patient's symptoms as due to atopy or otherwise without performing other troublesome investigations. It is an easy, less time consuming and reliable technique.

Double-Blind Method↗