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Biomedical subjects

M H Pereira

Publications and source records attributed to M H Pereira.

At least 19 recordsLinked to original sources
PubMed2006

RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection.

Mass sequencing of cDNA libraries from salivary glands of triatomines has resulted in the identification of many novel genes of unknown function. The aim of the present work was to develop a functional RNA interference (RNAi) technique for Rhodnius prolixus, which could be widely used for functional genomics studies in triatomine bugs. To this end, we investigated whether double-stranded RNA (dsRNA) can inhibit gene expression of R. prolixus salivary nitrophorin 2 (NP2) and what impact this might have on anticoagulant and apyrase activity in the saliva. dsRNA was introduced by two injections or by ingestion. RT-PCR of the salivary glands showed that injections of 15 microg of NP2 dsRNA in fourth-instar nymphs reduced gene expression by 75+/-14% and that feeding 1 microg/microL of NP2 dsRNA into second-instar nymphs (approx. 13 microg in total) reduced gene expression by 42+/-10%. Phenotype analysis showed that saliva of normal bugs prolonged plasma coagulation by about four-fold when compared to saliva of knockdown bugs. These results and the light color of the salivary gland content from some insects are consistent with the knockdown findings. The findings suggest that RNAi will prove a highly valuable functional genomics technique in triatomine bugs. The finding that feeding dsRNA can induce knockdown is novel for insects.

Animals↗
PubMed2004

Variability of the salivary proteins of 20 Brazilian populations of Panstrongylus megistus (Hemiptera: Reduviidae: Triatominae).

The objective of the present study was to study variability in the salivary proteins of 20 Panstrongylus megistus populations from different ecotopes and verify whether this variability influenced the intensity of the response to specific anti-saliva antibodies. Electrophoretic analysis of P. megistus saliva showed a complex protein composition and great interpopulation variability. A higher concentration of bands was observed in the 17-29 kDa region. The phenogram constructed from the electrophoretic profiles of the P. megistus study populations revealed the existence of two main groups. However, there was no evident relationship between these groups and geographical regions, ecotopes or hosts. Saliva inoculated by P. megistus during feeding elicited production of low level of anti-saliva antibodies in rabbit. The homologous and heterologous salivary proteins were recognised by serum of rabbit sensitised with saliva from only one population. Qualitative and quantitative differences were observed for recognised bands in the saliva of all eight populations studied by Western blot analysis. The most recognised bands were those of greatest molecular weight (68.0-97.4 kDa).

Animals↗
PubMed2003

Anti-complement activity in the saliva of phlebotomine sand flies and other haematophagous insects.

The saliva of haematophagous insects has a series of pharmacological activities which may favour blood feeding. In the present study, an inhibitory effect on the complement system was observed in salivary extracts obtained from the phlebotomine sand flies Lutzomyia longipalpis and Lu. migonei. Saliva from Lu. longipalpis was capable of inhibiting both the classical and alternative pathways, while that from Lu. migonei acted only on the former. Other haematophagous insect species were screened for inhibition of the classical pathway. The triatomine bugs Panstrongylus megistus, Triatoma brasiliensis and Rhodnius prolixus were also able to inhibit the classical pathway whereas the mosquito Aedes aegyti and flea Ctenocephalides felis were not. The activity of Lu. longipalpis saliva on the classical pathway was partially characterized. The inhibitor is a protein of Mr 10000-30000 Da, which is very resistant to denaturation by heat. The inhibition of the complement system by phlebotomine sand flies may have a role in the transmission of Leishmania to the vertebrate hosts. The inhibitor molecule is thus a promising component of a vaccine to target salivary immunomodulators.

Animals↗
PubMed2002

Molecular cloning and sequencing of salivary gland-specific cDNAs of the blood-sucking bug Triatoma brasiliensis (Hemiptera: Reduviidae).

Haematophagous insects produce pharmacological substances in their saliva to counteract vertebrate host haemostasis events such as coagulation, vasoconstriction and platelet aggregation. To investigate the bioactive salivary molecules of the triatomine bug Triatoma brasiliensis, we produced subtraction-enriched cDNAs of salivary-gland specific genes using suppression subtractive hybridization. Six full-length differentially expressed cDNAs (Tb113, Tb125, Tb152, Tb169, Tb180 and Tb198) were selected, cloned and sequenced. Sequence similarity searches of the databases using the putative amino acid sequence of our clones gave the following results: Tb152 - Triabin, an antithrombin induced platelet aggregation factor found in salivary gland extracts of T. pallidipennis. Tb169 - Pallidipin, an anticollagen induced platelet aggregation factor also found in T. pallidipennis salivary homogenates. Tb180 - Procalin, the major allergen of T. protracta saliva. The other three salivary-gland specific cDNAs produced no obvious homologies. Comparison of these salivary gland-specific cDNAs of with those of other triatomines combined with functional studies using recombinant proteins will allow a better understanding of the co-evolutionary process occurring between these insects and their vertebrate hosts, and may also lead to the discovery of novel antihaemostatic agents.

Amino Acid Sequence↗
PubMed2000

Influence of the blood meal source on the development of Triatoma infestans, Triatoma brasiliensis, Triatoma sordida, and Triatoma pseudomaculata (Heteroptera, Reduviidae).

The objective of this study was to determine the influence of the blood meal source on the life cycle and reproductive development of female Triatoma infestans (Klug, 1834), Triatoma brasiliensis Neiva, 1911, Triatoma sordida (Stal, 1859), and Triatoma pseudomaculata Corrga & Espfinola, 1964. In all triatomine species studied the life cycle was shorter for the groups fed on mice than for those fed on pigeons, the range of differences being between 1.5 times (T pseudomaculata and T. infestans) and 2.4 times (T brasiliensis). The mortality rate of nymphs during the life cycle tended to be greater in insects fed on pigeons than in those fed on mice, the differences for T. brasiliensis being statistically significant. Females of T sordida and T pseudomaculata had a greater fecundity than those of T. infestans and T. brasiliensis independently of the blood meal source. The differences of fecundity observed probably reflect differences in the availability of blood in the silvatic ecotopes of these species, meals being more frequent for T. infestans and T brasiliensis, which live at high densities in association with rodents in highly stable ecotopes. Because T. sordida and T. pseudomaculata live in more unstable ecotopes with fewer sources of blood they form small sparse colonies and invest more energy in reproduction than maintenance.

Animal Feed↗
PubMed2000

Identification of morphologically similar Rhodnius species (Hemiptera: Reduviidae: Triatominae) by electrophoresis of salivary heme proteins.

We examined intraspecific variability in the genus Rhodnius using starch gel electrophoresis of salivary heme proteins. Salivary protein profiles of 8 Rhodnius species (R. prolixus, R. robustus, R. neglectus, R. nasutus, R. ecuadoriensis, R. pallescens, R. pictipes, and R. domesticus) were compared. All species could be distinguished by this technique. The greatest protein polymorphism was found in R. ecuadoriensis, R. nasutus, R. robustus, and R. pictipes, followed by R. prolixus, R. neglectus, R. pallescens, and R. domesticus. This approach was able to distinguish R. prolixus from R. robustus and R. neglectus from R. nasutus, species with extreme phenotypical similarity.

Animals↗
PubMed2000

[Biological potential of Triatoma brasiliensis].

Biological and physiological parameters of Triatoma brasiliensis, Triatoma infestans, and Triatoma pseudomaculata were studied and compared. T. brasiliensis, reared on mice, showed a faster evolutionary cycle from first stage to adult and higher fecundity, when compared to the other species. T. infestans showed the fastest blood intake in both hosts tested, followed by T. brasiliensis and T. pseudomaculata. Clotting tests using salivary gland extracts of T. brasiliensis presented intermediary values of anti-clotting activity when compared to T. infestans and T. pseudomaculata.

Animals↗
PubMed1999

Differences in saliva composition among three Brazilian populations of Panstrongylus megistus (Hemiptera, Reduviidae).

Comparisons of electrophoretic profiles of triatomines (Panstrongylus megistus) from the Brazilian states of Bahia (BA), Minas Gerais (MG) and Santa Catarina (SC) revealed differences in the composition of the saliva between the three populations. A phenogram constructed on the basis of the most representative electrophoretic banding patterns allowed the specimens tested to be divided into two groups. The BA and SC populations could be completely separated while some individuals from MG could be placed in each group. The BA population presented fewer bands than the MG and SC ones. The populations studied also differed with regard to their degree of association with human habitations (sylvatic, peridomiciliary and/or intradomiciliary) and our results suggest that the proteinaceous composition of the saliva of the three distinct populations of triatomines could be related to the hosts each is likely to encounter.

Animals↗
PubMed1999

Action of the saliva of Triatoma infestans (Heteroptera: Reduviidae) on sodium channels.

Saliva of Triatoma infestans (Klug) produced a progressive reduction in the amplitude of the compound action potential recorded from rat sciatic nerve. The saliva also inhibited the Na+ current on GH3 cells. The data demonstrate that the saliva of T. infestans has an inhibitory effect on Na+ channels. We conclude that this effect may decrease the generation and conduction of nerve action potential, thereby decreasing the sensitivity of the region in which the insect probes, in a manner similar to that of local anesthetics. This study demonstrates such activity in the saliva of hematophagous insects. The adaptive value of this activity is clear, because the quantity of blood obtained by triatomines is limited by the irritation caused during the feeding process.

Action Potentials↗
PubMed1998

Salivary heme proteins distinguish Rhodnius prolixus from Rhodnius robustus (Hemiptera: Reduviidae: Triatominae).

Rhodnius prolixus interpopulation variability was studied based on a new approach using salivary heme proteins (nitrophorins) electrophoresis in starch gel. We compared salivary proteins profiles of R. prolixus from three different laboratory colonies from Honduras, Venezuela, Brazil and Rhodnius robustus from Venezuela, constructing a UPGMA. The Honduran and Venezuelan populations could not be distinguished from each other, but the Brazilian population was well separated from the others. The high similarity between Honduran and Venezuelan specimens lends support to current theories that the Central American populations of R. prolixus may have been introduced from a Venezuelan origin. The low polymorphism shown by the Honduran specimens is in agreement with a possible founder effect. This new approach also distinguished R. prolixus populations from R. robustus, species with extreme phenotypical similarity.

Animals↗
PubMed1996

Anticoagulant activity of Triatoma infestans and Panstrongylus megistus saliva (Hemiptera/Triatominae).

Salivary gland extract of Triatoma infestans prolonged thrombin time, prothrombin time, and activated partial thromboplastin time, while salivary gland extract of Panstrongylus megistus delayed thrombin time and activated partial thromboplastin time. However, saliva of P. megistus prolonged activated partial thromboplastin time and saliva of T. infestans delayed activated partial thromboplastin time and prothrombin time. T. infestans saliva interferes particularly with activity of factor VIII (intrinsic pathway) and factor V (common pathway), but also affects other factors. Saliva of the triatomine species studied presented distinct SDS-PAGE profiles. These results demonstrate that there are differences in anticoagulant activity and protein composition of triatomine saliva.

Animals↗
PubMed1995

[AIDS: the view from Africa].

Epidemiological data on AIDS in several zones in Africa is presented. The clinical and diagnostic aspects of HIV infection in Africa, based on the definition of Bangui's AIDS (1986), are analysed and the criteria is presented. The treatment and follow-up of a patient with infusion by HIV in the African context is discussed. Reference is then made to some data on the status of AIDS in Angola.

Acquired Immunodeficiency Syndrome↗
PubMed1993

Effects induced by saliva of the aquatic hemipteran Belostoma anurum on the isolated guinea-pig heart.

1. The effects of the saliva of the aquatic hemipteran Belostoma anurum were investigated on the isolated guinea-pig heart, perfused with Locke solution by the Langendorff's method. 2. The electrocardiogram (bipolar lead), the contractile force (g) and coronary flow (ml/min) were simultaneously recorded. 3. The total protein present in the saliva of each belostomatid was 11.17 +/- 2.39 micrograms (N = 10), and SDS-PAGE of the saliva showed a complex protein composition (N = 10). 4. Bolus injections of 0.2 ml saliva obtained from 10 belostomatids elicited complex effects which were divided into two phases (N = 8): an initial phase characterized by sinus bradycardia and/or A-V block associated with a decrease in contractile force and an increase in coronary flow followed by a decrease and a second phase, 0.5-5 min after saliva injection, characterized by a progressive increase in resting tension (ventricular contracture). 5. Since pretreatment of the preparation with atropine or verapamil did not prevent the initial and the late effects (N = 12), we conclude that these effects are not explained either by a muscarinic effect or by a stimulation of calcium channels. 6. Injection of saliva previously mixed with heparin (1000 IU/ml, N = 7), evoked the first but not the second phase (ventricular contracture). 7. Injection of saliva from two belostomatids into the isolated hearts of guinea pigs, not previously treated with heparin, elicited dramatic effects, such as ventricular contracture (N = 6). 8. We suggest that the formation of an acid-base complex (heparin-saliva) would prevent, in part, the toxic effects of the saliva on the heart.

Animals↗
PubMed1990

Return ventricular rhythm: new mechanism for tachyarrhythmia.

A case study is presented in which the electrocardiographic patterns during tachycardia showed ventricular premature depolarizations with retrograde conduction to the atria probably using a left-sided anomalous pathway and reentry to the ventricles with normal ventricular activation and duplication of the heart rate. This type of tachycardia is being labeled "return ventricular rhythm" and, as far as it is known, this is the first description of its mechanism.

Aged↗
PubMed1987

Standard score assessment on physique and performance of Brazilian athletes in a six tiered competitive sports model.

The use of large cross-sectional norms on Brazilian children and youths aged 7 to 18 years within a six level competition plan helps to assess development status and monitor change. Non-athletic prototypes for comparative purposes are illustrated by the use of the lowest competitive level of 18 year olds to assess differences from internationally elite players. The purpose of this study was to establish the efficacy of this practice in male and female basketball and volleyball samples. Comparisons were made on 11 anthropometric and performance variables using percent difference (% delta) and z-score values. The z-scores were highest for height, weight, and jumping ability in both sports groups. The volleyball players were the more linear in physique and the better jumpers. Related to their prototypes the female basketball players had the highest estimated VO2 max (ml kg-1 min-1), and the best values of anaerobic power measures. It is concluded that differences in physique and performance at various levels of competition compared to non-athletic prototypes may be used to infer selective and training factors.

Adolescent↗
PubMed1987

Hemodynamic effects of hypertonic sodium chloride during surgical treatment of aortic aneurysms.

Thoracoabdominal aortic surgery with aortic clamping is normally associated with major volemic shifts when the clamp is removed. This study compares the hemodynamic effects of hypertonic (HS) and isotonic (IS) solutions of sodium chloride (NaCl) infusions on the severe hypotension which follows aortic unclamping. Five consecutive patients received HS, and five received IS immediately after aortic unclamping. Blood loss, diuresis, and blood and crystalloid infusions were monitored throughout the operation. Blood gases, and pH, and hematocrit, hemoglobin, and plasma electrolyte levels were controlled during and 24 hours after surgery. Systemic arterial pressure, pulmonary arterial and wedge pressures, cardiac output, and systemic and pulmonary vascular resistance were monitored at the start of the operation, during aortic clamping, immediately after unclamping, immediately after HS or IS NaCl infusion and at the end of the operation. Patients who received HS NaCl exhibited higher pulmonary arterial and wedge pressures, higher cardiac index, and lower systemic and pulmonary vascular resistances immediately after the infusion. These patients required less volume transfusion than patients who received IS NaCl, despite slightly higher blood losses. It is concluded that HS NaCl is useful for the treatment of human hemorrhagic shock.

Adult↗