Biomedical subjects

M H Rao

Publications and source records attributed to M H Rao.

11 recordsLinked to original sources

PubMed2002

Existing record keeping system in government teaching hospitals of Karachi.

OBJECTIVE: To determine the deficiencies in the existing record keeping system (ERKS) in government teaching hospitals of Karachi. STUDY DESIGN: Descriptive, qualitative study to determine the deficiencies in the ERKS in government teaching hospitals of Karachi. SETTING: The study was conducted in four government teaching hospitals of Karachi from 1.1.1998 to 1.11.1998. METHODS: A total of 9725 pre-coded proformas were filled from 136 units of all hospitals including out patient departments (OPD), wards and emergency units. RESULTS: Majority of the records (52.2%) were retrievable within one hour. The mean +/- S.D of patients seen per day at all hospitals were 122.49 +/- 92.25 (57 OPDs), 30.04 +/- 16.24 (75 wards) and 186.25 +/- 145.80 (4 emergencies). In majority of units (57.4%), the stationary for record keeping system was either not provided or was not enough. Only 39.0% units have 75% or more required information on records. The important information (except age) i.e. temperature, pulse rate, blood pressure, diagnosis, weight, history of patient, laboratory findings, previous record and discharge report and prescribed doses of medicine were lacking. CONCLUSION: The ERKS in the government hospitals of Karachi is inadequate. It is therefore essential that uniform OPD, ward and emergency slips should be developed having all-important queries. So that data could be utilized for research purposes along with better planning and management of the hospital and procurement plan for the government.

Forms and Records Control↗

PubMed1995

Studies on conformational consequences of i to i + 3 side-chain cyclization in model cyclic tetrapeptides.

In an effort to explore the effect of ring size on the biologically active conformation of cyclic analogs of the mating pheromone alpha-factor (WHWLQLKPGQPMY) from Saccharomyces cerevisiae, eight cyclic tetrapeptides corresponding to the KPGQ portion of alpha-factor were synthesized. These N-alpha-acetyl/carboxyl amide terminal cyclic tetrapeptides were prepared on a 4-methylbenzhydrylamine resin using orthogonal Boc, Fmoc, OFm and OtBut protecting groups and HOBt-DIPC accelerated active esters or urethane-protected N-carboxyanhydrides. On-resin cyclization of the side-chain amino and carboxyl groups of the first and fourth residues, respectively, was performed with the BOP reagent to generate lactams containing 14-18 atoms. HF cleavage resulted in two products, the desired cyclic tetrapeptide and a major side product. All peptides were purified to near homogeneity (> 99%) by using reversed-phase HPLC and were characterized by FBMS and 1H NMR. Certain constrained cyclic tetrapeptides appear to be a mixture of isomers at room temperature as evidenced by HPLC and NMR. The major side product has been identified as a cyclo dimer, obtained as a consequence of interchain cyclization on the resin. CD analysis in several solvents gives evidence that some of the cyclic tetrapeptides exist in beta-turn conformations.

Amino Acid Sequence↗

PubMed1995

Expanded programme of immunization in Karachi.

Immunization status of children and their mothers and reasons for their failure to be immunized were studied. The study lasted for two months, i.e., August and September, 1993 at the Paediatric Outpatient Department of Civil Hospital, Karachi. Three groups of patients were targeted. Six hundred and twenty-four children under 1 year of age, 955 children between the ages of 1 to 5 years and 1579 mothers were registered. These women were questioned regarding their immunization status and that of their children. In the group below 1 year of age, BCG, DPT and polio (3 doses) and measles were given to 75%, 35% and 23% respectively. Tetanus toxoid (2 doses) was given to 47% of their mothers. In the group of children between 1-5 years, BCG, DPT and polio 3 doses and measles were given to 84%, 63% and 58% respectively. Tetanus toxoid (2 doses) were given to 64% of their mothers. The main reasons for not vaccinating were lack of information and lack of motivation.

Adult↗

PubMed1994

Antisense BCR-ABL oligomers cause non-specific inhibition of chronic myeloid leukemia cell lines.

We have examined the effects of antisense oligomers (AOs) of various lengths, sequences and chemistry on the proliferation of eight different cell lines, five derived from patients with chronic myelogenous leukemia (CML) and three from other sources. In general, phosphodiester AOs were inactive, presumably due to degradation by nucleases present in fetal calf serum. Both BA2 and B3A2 phosphorothiolate AOs (but not corresponding sense oligomers) significantly inhibited the proliferation of three CML cell lines (BV173, LAMA84, and KYO1), but the effect was independent of the type of breakpoint expressed by each cell line, suggesting that the inhibition was sequence dependent but not sequence specific. The CML cell lines tested showed different sensitivities to inhibition of proliferation by AOs--lines with defective expression of the normal ABL protooncogene (e.g. BV173) were more readily inhibited than lines with a normal ABL message (e.g. K562). We conclude that further studies are necessary to delineate the precise mechanism(s) by which CML cell proliferation is inhibited by AOs.

Base Sequence↗

PubMed1994

A change of Plasmodium species infecting children in Karachi over the last decade.

Febrile children of both sexes, aged between 0-15 years coming to the Civil Hospital over the last 12 years had their blood tested for malarial parasite. Five hundred and twenty-six slides positive for Plasmodium were analysed for species and stage identification. Of the total 59.3% children with malaria were between 5-15 years of age. Applying the test of proportion, Plasmodium vivax was the predominant species (P < 0.01) between 1981-1985 and plasmodium falciparum between 1986-1990. This trend persisted upto 1992. As malaria due to Plasmodium falciparum is more severe with multiple complications accurate and easier methods of its diagnosis are needed at primary health care level.

Adolescent↗

PubMed1987