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Biomedical subjects

M H Read

Publications and source records attributed to M H Read.

15 recordsLinked to original sources
PubMed2002

An intestinal obstruction in an eight-month-old child suffering from mevalonic aciduria.

UNLABELLED: This report describes a case of mevalonate kinase deficiency diagnosed at 1 mo of age. Soon after delivery, symptoms were suggestive of congenital infection. An intestinal occlusion occurred towards the age of 8 mo. CONCLUSION: Mevalonate kinase deficiency has variable clinical and biological signs which can lead to a delay in diagnosis. This is the first reported occurrence of bowel obstruction in this disease and the resemblance to a congenital infection in the neonatal period must be emphasized.

Amino Acid Metabolism, Inborn Errors↗
PubMed1997

Adolescent male athletes: body image, diet, and exercise.

The purpose of this study was to investigate and compare body image concerns, attitudes toward eating/weight control, and reasons for exercising between two groups of adolescent male athletes--football players (N = 44) and cross-country runners (N = 30). Subjects responded to surveys covering eating attitudes, weight concerns, physical traits, perceived and ideal body shape/size, and reasons for exercising. Significant differences were noted: Football players reported a more positive body image; cross-country runners indicated a greater degree of body dissatisfaction, more disordered eating patterns, and a greater degree of concern for weight control which identified this group as one in need of increased health education.

Adolescent↗
PubMed1989

Demographic profile and nutrient intake assessment of individuals using emergency food programs.

A dietary assessment of food pantry and soup kitchen users was conducted. A demographic and socioeconomic profile of participants was developed. One hundred ninety-one persons completed 30- to 40-minute interviews while in line to receive food assistance. Dietary status was determined from 24-hour recall data. Intakes of calcium, iron, thiamin, riboflavin, niacin, protein, vitamin A, and vitamin C were compared with the 1980 RDAs for age and sex. A dietary adequacy score was developed to evaluate nutrient quality by calculation of a nutrient density ratio (total intake for a nutrient divided by the RDA). A value of one was considered the norm. A score of 0.67 or less was considered an inadequate nutrient intake. Sixty-eight percent of the sample demonstrated some degree of inadequate nutrient intake. Nutrients that tended to be the lowest were calcium (76% inadequate), vitamin C (74% inadequate), and thiamin (74% inadequate). Inadequate nutrient intake was significantly associated with a lack of cooking facilities, lower monthly incomes, and lesser amounts of money spent on food. Comparison of food pantry users with soup kitchen participants indicated calcium was a problem nutrient for both groups.

Eating↗
PubMed1988

Adolescent compliance with dietary guidelines: health and education implications.

Through school-sponsored health fairs, 398 adolescents (153 males and 245 females) between the ages of 14 and 18 completed a questionnaire on their compliance with six of the U.S. Dietary Guidelines. Results indicated that adolescents report highest compliance on "eat a variety of foods" and "eat foods with adequate starch and fiber." Conversely, they indicated low compliance on "reduce sugar intake" and "reduce fat, saturated fat and cholesterol intake." Sex differences were noted on two dietary guidelines. Females reported lower compliance with respect to "maintain desirable body weight." Males, on the other hand, reported lower compliance than females on "reduce salt intake." Given the fact that the dietary guidelines of "reduce fat, saturated fat and cholesterol," "maintain desirable body weight," and "reduce salt intake" are all aimed at reducing cardiovascular risk, the low compliance reported by adolescents may have long-range health implications.

Adolescent↗
PubMed1982

[Assay of total serum bile acids. Reference values in children].

Serum total bile acids were measured using an enzyme micromethod involving 3-hydroxysteroid dehydrogenase (Sterognost 3 Fluorometry) without prior extraction. The test specimen was 50 microliters of serum, with reading by spectrofluorometry. The degree of accuracy assessed at several concentration levels was acceptable for values greater than 5 mumol/l. The calibration function was linear between 0 and 80 mumol/l. Lower limit of detection was 0.88 mumol/l. Results given by this method were compared with those obtained using the Schwarz technique involving extraction of bile acids and their enzyme estimation. The correlation between the two methods was satisfactory. Absence of interference by bilirubin was confirmed. Total bile acids were measured in the serum of fasting children, aged from 1 day to 4 years. High values were seen at birth (m = 8.33 mumol/l, SD = 3.46, n = 10) as well as during the first month of life. Serum total bile acid concentration decreased up to the age of 10 months (m = 2.13 mumol/l, SD = 1.91, n = 31). After one year, they increased progressively, reaching at the age of 4 values similar to those in the adult (m = 3.80 mumol/l, SD = 2.17, n = 30). Particularly high values were found in children with delayed growth or in premature infants (m = 19.66 mumol/l, SD = 10.4, n = 15). Serum total bile acid levels were lower in umbilical cord blood and in the newborn. The increase in serum bile acids found in the newborn may be due to slight cholestasis or to a reduction in the hepatic clearance, showing the special type of hepatic function in the young child.

3-Hydroxysteroid Dehydrogenases↗
PubMed1982

Food supplement usage by the elderly.

A variety of food supplements were taken by 66 percent of the elderly persons in this study. Ascorbic acid and vitamin E were the most popular choices. Some supplements may have improved nutritional status, while others were probably inappropriate or unnecessary. The findings indicate that the elderly have a number of erroneous beliefs about the efficacy of food supplements. Reasons for supplementation were often inappropriate, and unreliable sources of information were used. In addition, substantial amounts of money were spent on food supplements by those already existing on a limited budget. Nutrition education is needed to demonstrate the relationship between adequate diet, good health, and appropriate versus inappropriate food supplement consumption.

Aged↗
PubMedDate not supplied

[Plasma homocysteine measurement: a study of pre-analytical variation factors for conditions for total plasma homocysteine concentration].

An increase in homocysteine, a sulphur amino acid, is nowdays considered as a risk factor for cardiovascular diseases, and is independent of other risk factors. Reference range for total plasma homocysteine level in adults is usually 5-15 mmol/l. Hyperhomocysteinemia is defined as a fasting total plasma homocysteine level > 15 mmol/l. There may be also graded increased risks for subjects with homocysteinemia from 10 to 15 mmol/l. However, no threshold has been defined, partly because of the lack of standardization in pre-analytical and analytical steps. The aim of the present work was to evaluate three pre-analytical parameters on plasma homocysteine levels: i) the influence of three anticoagulants (EDTA, sodium citrate and lithium heparin); ii) the delay period of blood sample on ice before centrifugation; and iii) the advantages of strong acidic citrate at room temperature. The mean concentrations of total plasma homocysteine were different in function of the anticoagulant. These differences (EDTA minus lithium heparin or EDTA minus sodium citrate) were less than 10% however the used methods and could explain the good correlation between the results. However we recommend to keep the anticoagulant constant in the same study. When EDTA blood samples were immediately put on crushed ice, the maximum delay period before centrifugation could reach 4 hours. If ice is unavailable, strong acidic citrate at room temperature is a good alternative until for 4 hours.

Anticoagulants↗
PubMedDate not supplied

[Comparison of plasma total homocysteine determinations in 9 French hospital laboratories by using 6 different methods].

A lot of methods are now available for total plasma homocysteine (tHcy) determination. Commercial kits using immunoassay, easier to use, begin to supplant in-house laboratory methods. Our aim is to evaluate the interchangeability of tHcy measurements in 9 French hospital laboratories. Six different method types were used: 2 gas chromatography-mass spectrometry (GC-MS), 2 HPLC with fluorescence detection subdivided in one in-house method and one commercial kit (Bio-Rad ), 3 fluorescence polarization immunoassays (FPIA), 1 enzyme immunoassay, 1 amino acid analyser, 1 capillary electrophoresis coupled with laser-induced fluorescence detection (EC-LIF). Each laboratory analysed 41 patient's plasma samples in which 8 samples contained added homocystine. Results were analysed for imprecision, recovery, and methodological differences. The mean among-laboratory imprecision (CV) ranged from 12.5 to 18% in function of plasma sample type and was identical to the mean among-method variation. In terms of recovery, we obtained underestimated results with immunoassays. The bias relative to the GC-MS method was less than 12.5% except for two laboratories, one using FPIA assay and the other EC-LIF. In conclusion, the interchangeability of tHcy results between laboratories is not satisfactory and does not allow us to evaluate cardiovascular risk linked to moderate increases of tHcy.

Blood Chemical Analysis↗