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M HARBOE

Publications and source records attributed to M HARBOE.

At least 19 recordsLinked to original sources
PubMed1965

PROPERTIES OF VARIOUS ANTI-GAMMA-GLOBULIN FACTORS IN HUMAN SERA.

The serological and physicochemical properties of the following three forms of human anti-gamma-globulin factors were compared: (a) rheumatoid factors; (b) Milgrom type anti-gamma-globulin factors; and (c) factors directed against an antigen in human gammaG-globulin that is hidden in the intact molecule and revealed by enzymatic digestion at low pH. The property common to these factors is ability to interact with human gammaG-globulin; they are distinguishable because they react with different antigenic groups on this molecule. In all of five sera, the Milgrom type anti-gamma-globulin factors were gammaM-globulins. They reacted with various human gammaG-globulin antibodies but failed to interact with gammaM-globulin type antibodies in agglutination and absorption experiments. When isolated from other anti-gamma-globulin factors, they agglutinated red cells coated with intact anti-Rh antibodies, but failed to react with cells cells coated with pepsin-digested anti-Rh antibody. These observations indicate that the agglutinator reacts with the crystallizable, inert fragment of gammaG-globulin. Anti-gamma-globulin activity directed against an antigen in human gammaG-globulin revealed by pepsin digestion was demonstrated in gammaG-, gammaA-, and gammaM-globulins. This anti-gamma-globulin factor could be absorbed by antigen-antibody precipitates containing human antibody, which shows that the hidden antigen in human gammaG-globulin is revealed not only by enzymatic digestion at low pH, but also when gammaG-globulin is present as antibody in an antigen-antibody precipitate. Rheumatoid factors and Milgrom type anti-gamma-globulin factors were also absorbed by antigen-antibody precipitates containing human antibody. The results indicate that the three distinct forms of antigamma-globulin factors may all be produced as a result of antigenic stimulation by autologous antigen-antibody complexes.

Antibodies↗
PubMed1962

Genetic characters of human gamma-globulins in myeloma proteins.

The genetic factors Gm(a), Gm(b), Gm(x), and Inv(a), Inv(b) described for normal human gamma-globulin were all found in different myeloma proteins. A single myeloma protein never contained more than one product of alternate alleles even in heterozygous individuals. However, factors determined by the two different loci were often found in the same myeloma protein. The Gm(a) character of the myeloma protein parallelled that of the normal gamma-globulin of the same serum in most cases. In contrast, the Gm(b) character was usually absent in the myeloma protein when it was directly demonstrable in the normal gamma-globulin. The myeloma proteins from six Negroes were Gm(a+b-), whereas the normal gamma-globulin was Gm(a+b+). This indicates that the effect of gene Gm(b) is similar in Negroes and whites, even though its relation to gene Gm(a) is different in the two races. Gm factors were found only in the 7S gamma-globulin type myelomas and not in other products of plasma cell tumors. Inv characters were, however, present in all four types of proteins studied, namely 7S and 19S gamma-globulins, beta(2A)-globulins, and Bence Jones proteins. In two instances, genetic heterogeneity of the protein products was demonstrated suggesting the proliferation of more than one clone of plasma cells in some multiple myeloma patients. The accumulated evidence obtained in this study strongly suggested that the presence and absence of genetic characters was compatible with the concept that myeloma proteins were closely analogous to individual moieties in the spectrum of normal gamma-globulins rather than truly abnormal proteins. Their study offered evidence of a heterogeneity of genetic characters among the normal gamma-globulins in a given individual. It also appears probable that in normal individuals single plasma cells have a restricted capacity to express genetic information in their protein product.

Bence Jones Protein↗
PubMed1962

Localization of two genetic factors to different areas of gamma-globulin molecules.

Gm(a) and Gm(b) factors are present in 7S gamma-globulin molecules and absent in 19S gamma-globulins, beta(2A)-globulins and Bence-Jones proteins, whereas the Inv(b) factor was demonstrated in all four kinds of proteins. The Inv type was identical in isolated 7S and 19S gamma-globulins of six normal sera. After papain splitting of 7S gamma-globulin, Gm determining sites were present only in the fast (F) split product and Inv determining sites were present only in the slow (S).

Bence Jones Protein↗