Biomedical subjects

M Hager

Publications and source records attributed to M Hager.

At least 19 recordsLinked to original sources

PubMed1999

Targeted inactivation of the smallest plastid genome-encoded open reading frame reveals a novel and essential subunit of the cytochrome b(6)f complex.

The smallest conserved open reading frame in the plastid genome, ycf6, potentially specifies a hydrophobic polypeptide of only 29 amino acids. In order to determine the function of this reading frame we have constructed a knockout allele for ycf6. This allele was introduced into the tobacco plastid genome by chloroplast transformation to replace the wild-type ycf6 allele. Homoplasmic Deltaycf6 plants display a photosynthetically incompetent phenotype. Whereas the two photosystems are intact and physiologically active, we found that the electron transfer from photosystem II to photosystem I is interrupted in Deltaycf6 plants. Molecular analyses revealed that this block is caused by the complete absence of the cytochrome b(6)f complex, the redox-coupling complex that interconnects the two photosystems. Analysis of purified cytochrome b(6)f complex by mass spectroscopy revealed the presence of a protein that has exactly the molecular mass calculated for the Ycf6 protein. This suggests that Ycf6 is a genuine subunit of the cytochrome b(6)f complex, which plays a crucial role in complex assembly and/or stability. We therefore propose to rename the ycf6 reading frame petN.

Amino Acid Sequence↗

PubMed1998

The WHO has seen the future, and it's full of good health.

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Acquired Immunodeficiency Syndrome↗

PubMed1998

Do you have hepatitis C?

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Blood Banks↗

PubMed1998

Protective effects of fruits and vegetables against in vivo clastogenicity of cyclosphosphamide or benzo[a]pyrene in mice.

Seven fruits and 10 vegetables commonly consumed in Germany were investigated for their anticlastogenic potencies against cyclophosphamide (CP) and benzo[a]pyrene (BaP) in the in vivo mouse bone marrow micronucleus assay. We detected protective effects in 76.5% and 70.6% of the samples, respectively, and more or less distinct quantitative differences between the various plant materials and the two clastogens investigated. With respect to CP, moderate activities were exerted by sweet cherries, strawberries, cucumber, radish and tomatoes, average activities by bananas, oranges, peaches, asparagus and red beets and strong activities by yellow red peppers and especially spinach. Apples (cultivar Jona Gold), brussels sprouts, cauliflower and onions were inactive. With respect to BaP, we found moderate activities in strawberries, brussels sprouts and radish, average activities in sweet cherries, oranges, peaches, asparagus, red beets, cucumber and spinach and strong activities in bananas and kiwi. Apples, cauliflower, onions, tomatoes and yellow-red peppers were inactive. When oranges were fractionated according to previously described schemes (Edenharder et al., 1995), anticlastogenic activities against CP were exerted by materials extracted with n-hexane, acetone and 2-propanol and in the terminal residue, but not in the dichloromethane and water phases. With respect to BaP, materials extracted with acetone showed strong anticlastogenicity while the 2-propanol fraction, the aqueous phase and the terminal residue were less potent. The n-hexane and the dichloromethane fractions were inactive. In red beets, all fractions showed anticlastogenicity against CP and BaP as well. However, the n-hexane and dichloromethane fractions were most potent with respect to CP, while for BaP the aqueous phase and the terminal residue were most effective. These result suggest the presence of various (groups of) anticlastogenic compounds with different chemical structure.

Animals↗

PubMed1997