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Biomedical subjects

M Hajek

Publications and source records attributed to M Hajek.

At least 19 recordsLinked to original sources

Novel shielding materials for space and air travel.

The reduction of dose onboard spacecraft and aircraft by appropriate shielding measures plays an essential role in the future development of space exploration and air travel. The design of novel shielding strategies and materials may involve hydrogenous composites, as it is well known that liquid hydrogen is most effective in attenuating charged particle radiation. As precursor for a later flight experiment, the shielding properties of newly developed hydrogen-rich polymers and rare earth-doped high-density rubber were tested in various ground-based neutron and heavy ion fields and compared with aluminium and polyethylene as reference materials. Absorbed dose, average linear energy transfer and gamma-equivalent neutron absorbed dose were determined by means of LiF:Mg,Ti thermoluminescence dosemeters and CR-39 plastic nuclear track detectors. First results for samples of equal aerial density indicate that selected hydrogen-rich plastics and rare-earth-doped rubber may be more effective in attenuating cosmic rays by up to 10% compared with conventional aluminium shielding. The appropriate adaptation of shielding thicknesses may thus allow reducing the biologically relevant dose. Owing to the lower density of the plastic composites, mass savings shall result in a significant reduction of launch costs. The experiment was flown as part of the European Space Agency's Biopan-5 mission in May 2005.

Aircraft↗

Dose distribution in the Russian Segment of the International Space Station.

Absorbed dose and average linear energy transfer (LET) were assessed by means of (7)LiF:Mg,Ti (TLD-700) thermoluminescent (TL) detectors for different panels on-board the Russian Segment of the International Space Station in the timeframe between March and November 2002 (233 d). A technique is presented to correct the measured absorbed dose values for TL efficiency in the radiation climate on-board the spacecraft. Average LET is determined from the high-temperature TL emission in the TLD-700 glow curve and used as a parameter in the TL efficiency correction. Depending on the shielding distribution, the efficiency-corrected absorbed dose varies between 154 +/- 5 microGy d(-1) in panel no. 327 (core block ceiling) and 191 +/- 3 microGy d(-1) in panel no. 110 (core block central axis, floor). The experimental data are compared with the model calculations by using detailed shielding distributions and orbit parameters as inputs.

Algorithms↗

Error images for spectroscopic imaging by LCModel using Cramer-Rao bounds.

The results of spectroscopic imaging (SI) measurements are often presented as metabolic images. If the spectra quality is not sufficient, the calculated concentrations are biased and the metabolic images show an incorrect metabolite distribution. To simplify the quality analysis of spectra measured by SI, an error image, reflecting the accuracy of the computed concentrations, can be displayed along with the metabolite image. In this paper the relevance of Cramer-Rao bounds (CRBs) calculated by the LCModel program to describe errors in estimated concentrations is validated using spectra simulations. The relation between the average CRBs and standard deviations (STD) of metabolite concentrations from 100 simulated spectra for various signal to noise ratio and line broadening conditions is evaluated. A parameter for calculating error images for metabolite ratios is proposed and an effective way to display error images is shown. The results suggest that the average CRBs are strongly correlated with the standard deviations and hence that CRB values reflect the relative uncertainty of the calculated concentrations. The error information can be integrated directly into a metabolite image by displaying only those areas of the metabolite image with corresponding CRBs below a selected threshold or by mapping CRBs as a transparency of the metabolite image. The concept of error images avoids extensive examination of each SI spectrum and helps to reject low quality spectra.

Algorithms↗

Magnetic resonance imaging of intrahepatically transplanted islets using paramagnetic beads.

Superparamagnetic agents can be reliably used for magnetic resonance imaging (MRI) of pancreatic islets located in the liver sinusoids. However, the main disadvantages seemed to be the rather long culture time necessary for islet labeling and the low specificity of these agents. In the present study we investigated a more specific approach with a shorter labeling time using immunomagnetic particles. Isolated rat islets were cultivated with immunomagnetic beads coated with antibody against rat MHC class I antigen. Labeled islets were transplanted into the livers of syngeneic rats. The animals were examined weekly by MRI or livers explanted 10 minutes after islet transplantation for in vitro experiments. In both in vitro and in vivo studies, labeled transplanted islets were imaged as hypointensive spots, diffusely distributed throughout the liver. This experiment represents an alternative way of islet imaging by magnetic resonance, which is as effective as the use of known superparamagnetic contrast agents and more specific owing to targeting to specific donor antigens.

Animals↗

Austrian dose measurements onboard space station MIR and the International Space Station--overview and comparison.

The Atominstitute of the Austrian Universities has conducted various space research missions in the last 12 years in cooperation with the Institute for Biomedical Problems in Moscow. They dealt with the exact determination of the radiation hazards for cosmonauts and the development of precise measurement devices. Special emphasis will be laid on the last experiment on space station MIR the goal of which was the determination of the depth distribution of absorbed dose and dose equivalent in a water filled Phantom. The first results from dose measurements onboard the International Space Station (ISS) will also be discussed. The spherical Phantom with a diameter of 35 cm was developed at the Institute for Biomedical Problems and had 4 channels where dosimeters can be exposed in different depths. The exposure period covered the timeframe from May 1997 to February 1999. Thermoluminescent dosimeters (TLDs) were exposed inside the Phantom, either parallel or perpendicular to the hull of the spacecraft. For the evaluation of the linear energy transfer (LET), the high temperature ratio (HTR) method was applied. Based on this method a mean quality factor and, subsequently, the dose equivalent is calculated according to the Q(LET infinity) relationship proposed in ICRP 26. An increased contribution of neutrons could be detected inside the Phantom. However the total dose equivalent did not increase over the depth of the Phantom. As the first Austrian measurements on the ISS dosimeter packages were exposed for 248 days, starting in February 2001 at six different locations onboard the ISS. The Austrian dosimeter sets for this first exposure on the ISS contained five different kinds of passive thermoluminescent dosimeters. First results showed a position dependent absorbed dose rate at the ISS.

Astronauts↗

Passive in-flight neutron spectrometry by means of bonner spheres.

The spectral distribution of the neutron fluence rate was determined on-board a series of eight trans-atlantic flights between Cologne (Germany) and Washington, DC (USA), using a passive Bonner Sphere spectrometer based on thermoluminescence detectors. Contrary to the commonly applied active systems, the passive instrument facilitates a complete discrimination of gamma ray and charged particle-induced events in the detector count rate. The system was calibrated in the CERN-EU High-Energy Reference Field (CERF). The measured spectra are compared with FLUKA Monte Carlo simulations and show excellent agreement.

Aircraft↗

A TLD-based personal dosemeter system for aircrew monitoring.

Evaluation of LiF:Mg,Ti thermoluminescence dosemeters (TLDs) according to the high-temperature ratio (HTR) method enables the determination of the dose-average linear energy transfer (LET), the mean quality factor and the dose equivalent in mixed radiation fields of unknown composition. The neutron contribution is assessed by the Extended Pair method calibrated in the CERN-EU High-Energy Reference Field (CERF). The advantages of the small passive detectors as an easy-to-handle monitoring system for in-flight surveillance are demonstrated by measurements on-board north-bound and trans-equatorial flights. The experimental results are compared with calculations by the well-established CARI code.

Aircraft↗

Analysis of the neutron component at high altitude mountains using active and passive measurement devices.

The European Council directive 96/29/Euratom requires dosimetric precautions if the effective dose exceeds 1 mSv/a. On an average, this value is exceeded by aircrew members. Roughly half of the radiation exposure at flight altitudes is caused by cosmic ray-induced neutrons. Active (6LiI(Eu)-scintillator) and passive (TLDs) Bonner sphere spectrometers were used to determine the neutron energy spectra atop Mt. Sonnblick (3105 m) and Mt. Kitzsteinhorn (3029 m). Further measurements in a mixed radiation field at CERN as well as in a proton beam of 62 MeV at Paul Scherrer Institute, Switzerland, confirmed that not only neutrons but also charged particles contribute to the readings of active detectors, whereas TLD-600 and TLD-700 in pair allow the determination of the thermal neutron flux. Unfolding of the detector data obtained atop both mountains shows two relative maxima around 1 MeV and 85 MeV, which have to be considered for the assessment of the biologically relevant dose equivalent. By convoluting the spectra with appropriate conversion functions the neutron dose equivalent rate was determined to be 150 +/- 15 nSv/h. The total dose equivalent rate determined by the HTR-method was 210 +/- 15 nSv/h. The results are in good agreement with LET-spectrometer and Sievert counter measurements carried out simultaneously.

Aerospace Medicine↗

Application of the high-temperature ratio method for evaluation of the depth distribution of dose equivalent in a water-filled phantom on board space station Mir.

A water-filled tissue equivalent phantom with a diameter of 35 cm was developed at the Institute for Biomedical Problems. Moscow. Russia. It contains four channels perpendicular to each other, where dosemeters can be exposed at different depths. Between May 1997 and February 1999 the phantom was installed at three different locations on board the Mir space station. Thermoluminescence dosemeters (TLDs) were exposed at various depths inside the phantom either parallel or perpendicular to the hull of the spacecraft. The high-temperature ratio (HTR) method was used for the evaluation of the TLDs. The method was developed at the Atominstitute of the Austrian Universities. Vienna, Austria, and has already been used for measurements in mixed radiation fields on earth and in space with great success. It uses the changes of peak height ratios in LiF:Mg,Ti glow curves in dependence on the linear energy transfer (LET), and therefore allows determination of an 'averaged' LET as well as measurement of the absorbed dose. A mean quality factor and, subsequently, the dose equivalent can be calculated according to the Q(LETinfinity) relationship proposed by the ICRP. The small size of the LiF dosemeters means that the HTR method can be used to determine the gradient of absorbed dose and dose equivalent inside the tissue equivalent body.

Aerospace Medicine↗

Dose assessment of aircrew using passive detectors.

Radiation exposure of aircrew is a serious concern which has been given special emphasis in the European Council directive 96/29/Euratom. The cosmic ray induced neutron component can contribute more than 50% to the biologically relevant dose at aviation altitudes. Various computational approaches to route dose assessment, e.g. CARI, are in use nowadays and are compared with experimental data. Measurements of aircrew exposure usually involve extensive instrumentation in order to cover the whole particle spectrum and energy range present inside aircraft. Due to their small size and easy handling, thermoluminescence dosemeters represent an appropriate alternative. Previous measurements onboard aircraft applying the high-temperature ratio method with LiF:Mg,Ti dosemeters for the determination of an 'averaged' linear energy transfer of mixed radiation fields demonstrate the ability of this method to evaluate the dose equivalent, according to the Q(LETinfinity) relationship proposed by the ICRP. Measurements with CaF2:Tm dosemeters are currently in progress and are discussed here.

Aerospace Medicine↗

Advantages of passive detectors for the determination of the cosmic ray induced neutron environment.

Due to the pronounced energy dependence of the neutron quality factor, accurate assessment of the biologically relevant dose requires knowledge of the spectral neutron fluence rate. Bonner sphere spectrometers (BSSs) are the only instruments which provide a sufficient response over practically the whole energy range of the cosmic ray induced neutron component. Measurements in a 62 MeV proton beam at Paul Scherrer Institute, Switzerland, and in the CERN-EU high-energy reference field led to the assumption that conventional active devices for the detection of thermal neutrons inside the BSS, e.g. 6Lil(Eu) scintillators, also respond to charged particles when used in high-energy mixed radiation fields. The effects of these particles cannot be suppressed by amplitude discrimination and are subsequently misinterpreted as neutron radiation. In contrast, paired TLD-600 and TLD-700 thermoluminescence dosemeters allow the determination of a net thermal neutron signal.

Aerospace Medicine↗

Thermoluminescence dating of archaeological artefacts from the Middle Neolithic, Bronze Age and the Roman Empire period.

Thermoluminescence (TL) dating was applied for artefacts found near the small village of Michelstetten, Lower Austria. Settlements in this region can be traced hack a long time and, according to archaeologists, the discovered artefacts may be as old as 6000 years. A modified sample preparation technique based on the fine-grain method was developed. This technique results in a higher reproducibility and reduces the overall preparation time. For some artefacts the new information of the TL dating leads to an unforeseen re-interpretation of the archaeological age. Furthermore, an iron furnace from the period of the Roman Empire could be dated. For the first time, it was possible to estimate correctly the point of time of the burn-down of an ancient wooden house via an analysis of the house's clay plaster. The fire took place in the sixth century; this was confirmed by dating ceramic artefacts.

Archaeology↗

Isolation and structure elucidation of Chlorofusin, a novel p53-MDM2 antagonist from a Fusarium sp.

Wild-type p53 plays a crucial role in the prevention of cancer. Since dysfunction of p53 can be caused by increased levels of the protein MDM2, small molecules which antagonize the interaction between these two proteins have potential in cancer therapy. The discovery and structure determination of a fungal metabolite, chlorofusin, which antagonizes the p53/MDM2 interaction are reported.

Antineoplastic Agents↗

A rapid and facile method for the dereplication of purified natural products.

A new approach to the use of commercial databases for the dereplication of purified natural products has been developed. This is based on searching a text file that links each structure with its molecular weight and an exact count of the number of methyl, methylene, and methine groups it contains. Analysis of such a text file, constructed from a database containing more than 126,000 natural product structures, revealed that these data, readily measured using MS and NMR spectroscopy, are highly discriminating. The identification of an alkaloid and a sesquiterpene using this new approach is described.

Alkaloids↗

Measurement of the depth distribution of average LET and absorbed dose inside a water-filled phantom on board space station MIR.

The Atominstitute of the Austrian Universities developed the HTR-method for determination of absorbed dose and "averaged" linear energy transfer (LET) in mixed radiation fields. The method was applied with great success during several space missions (e.g. STS-60, STS-63, BION-10 and BION-11) and on space station MIR in the past 10 years. It utilises the changes of peak height ratios in LiF thermoluminescent glowcurves in dependence on the LET. Due to the small size of these dosemeters the HTR-method can be used also for measurements inside tissue equivalent phantoms. A water filled phantom with a diameter of 35 cm containing four channels where dosemeters can be exposed in different depths was developed by the Institute for Biomedical Problems. This opens the possibility to measure the depth distribution of the average LET and the dose equivalent simultaneously. During phase 1 dosemeters were exposed for 271 days (05.1997-02.1998) in 6 different depths inside the phantom, which was positioned in the commander cabin. In phase 2 dosemeters were exposed in 2 channels in 6 different depths for 102 days (05.1998-08.1998) in the board engineer cabin, following an exposure in different channels in 3 different depths for 199 days (08.1998- 02.1999) in the Modul KWANT 2.

Humans↗

[Sex specific differences in hemispheric lateralization in schizophrenia? An MEG-MRI study].

In this magnetoencephalography study the issue of hemispheric lateralisation in patients with schizophrenia was addressed using acoustically evoked neuromagnetic fields. The characteristics of dipoles in the superior temporal gyrus, the primary auditory cortex, were calculated. In contrast to other studies, alterations did not concern the localisation, but rather the orientation of dipoles. Of pathophysiological interest was that the dipoles abnormalities were found left-hemispherically in male (p = 0.02) and right-hemispherically in female patients with schizophrenia (p = 0.01) when compared to controls. The findings suggest gender-specific alterations of hemispheric lateralisation in schizophrenia.

Adult↗

Selective amobarbital test for the determination of language function in patients with epilepsy with frontal and posterior temporal brain lesions.

PURPOSE: Selective amobarbital tests with selective temporary inactivation of the left frontal operculum and/or the left parietotemporal cortex were performed in 5 patients with left-hemispheric epileptogenic lesions in or adjacent to classical Broca's and/or Wernicke's area. The aim was to assess language functions in these brain regions before surgery, to tailor the surgery according to the individual functional importance of these brain regions, and to predict postoperative outcome. METHODS: Amobarbital was injected by transfemoral selective catheterization of the arteries supplying the target areas. Along with neuropsychological and neurological testing during the amobarbital procedure, EEG recordings were performed in all patients, and [99mTc]HMPAO-single photon emission computed tomography (SPECT) in 2 patients. RESULTS: After the amobarbital injection into the left frontal opercular region, there was no recognizable language dysfunction in 3 patients. In these 3 patients, the lesions in or adjacent to the frontal operculum were completely resected without postoperative language impairment. In the remaining 2 patients, temporary language impairment after the amobarbital injection into the left frontoopercular and Wernicke's region, respectively, suggested language functions in these areas. Surgery was restricted to the left mesiotemporal lobe in 1 patient. In the other patient, the tumor infiltrating the frontal operculum was restrictively resected. Postoperatively, the first patient had no language impairment, but the latter had transient global aphasia, from which she recovered. CONCLUSIONS: Selective temporary amobarbital inactivation of brain regions that may be associated with language has clearly indicated the presence or absence of language functions in these regions. The test contributed substantially to planning of the surgical approach in each patient. The predictive value of the amobarbital test was demonstrated by the postoperative outcome.

Adolescent↗