Biomedical subjects

M Heide

Publications and source records attributed to M Heide.

At least 19 recordsLinked to original sources

PubMed1991

Nucleotide sequence of pea seed-borne mosaic potyvirus coat protein gene.

cDNA of pea seed-borne mosaic potyvirus (PSbMV) RNA was synthesized and cloned in E. coli. Four overlapping clones that cover the complete PSbMV genome, except the extreme 5' terminus, were identified by restriction enzyme mapping, hybridization analysis, and partial sequencing. Overlapping cDNA clones covering 1386 nucleotides of the 3' terminus were sequenced. The nucleotide sequence contains one open reading frame (ORF), followed by an untranslated region of 163 nucleotides and a poly(A)-tract. The deduced amino acid sequence was found to include the C-terminus of the predicted RNA-dependent RNA polymerase and the coat protein. A glutamine-alanine dipeptide was identified as a putative 49-kD proteinase cleavage site at the N-terminus of the coat protein.

Amino Acid Sequence↗

PubMed1991

The complete nucleotide sequence of pea seed-borne mosaic virus RNA.

The complete nucleotide sequence of the RNA genome of pea seed-borne mosaic virus (PSbMV) was determined from cloned cDNA and by direct sequencing of viral RNA. The PSbMV genomic sequence was determined to be 9924 nucleotides in length excluding the poly(A) tract. The RNA contained an open reading frame (ORF) of 9618 nucleotides with the potential to encode a polyprotein with a calculated Mr of 364000 (364K). The ORF was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' untranslated region of 163 nucleotides. A comparison of the PSbMV polyprotein with the polyproteins of the potyviruses tobacco etch virus (TEV), tobacco vein mottling virus (TVMV), plum pox virus (PPV) and potato virus Y (PVY) showed that PSbMV had a similar genome organization. The polyproteins had a high level of amino acid identity except in the N-terminal region, which varied in both sequence and length. Putative proteolytic cleavage sites were identified in the polyprotein of PSbMV by comparison with those identified for other potyviruses. The cleavage site between the 6K protein and the 49K proteinase is proposed to occur at the C-terminal side of glutamic acid and not at the C-terminal side of glutamine as in other potyviruses. In addition to the five proteolytic cleavage sites for the 49K proteinase identified previously, a sixth putative cleavage site was identified internally in the 49K proteinase of PSbMV, as well as in the 49K proteinases of TEV, TVMV, PPV, PVY and soybean mosaic virus. Cleavage at this site in the 49K proteinases of TEV, TVMV and PPV would result in an N-terminal protein of 22K to 24K, which is similar in size to the size determined for their VPgs.

Amino Acid Sequence↗

PubMed1990

Purification of plant viruses and virus coat proteins by high performance liquid chromatography.

High performance liquid chromatography (HPLC) gel filtration has been successfully applied in the purification of elongated and isometric plant viruses. Two different approaches have been tested. In one approach, semi-purified virus particles were dissociated with lithium chloride and the released coat proteins purified by HPLC gel filtration. The purified coat protein was highly immunogenic and gave rise to very specific antisera reacting with intact virus particles as well as with SDS-denatured coat protein monomers. This method is generally applicable only for elongated viruses since many isometric viruses are not dissociated by the lithium chloride treatment. The second approach consisted in gel filtration of native, undissociated virus particles and could be used both with elongated and isometric viruses. Both methods were fast and simple to perform and removed all or most of the contaminating plant proteins as judged by sodium dodecylsulphate gel electrophoresis followed by silver staining or by immunoblotting with antiserum against healthy plant extracts. With both methods the recovery of virus coat protein was about 30% on average.

Animals↗

PubMed1990

[Therapeutic concept for fracture of the trochanter major in hip joint alloarthroplasty].

During the first two years following the foundation of the Orthopaedic Clinic of Frankfurt (O.) (1985/1986) occurred connected with the procedure of hip arthroplasty by reason of failing technical teamwork of the operation steam 20 fractures of the Trochanter major. By means of aetiological-biomechanical analysis and a roentgenological classification three groups of fractures which require different therapeutic measures are described. Experiences with our treatment and the development of this complication are demonstrated.

Aged↗

PubMed1985

[Changes in nutrition habits in obesity and hyperlipoproteinemia].

Lowering of cardiovascular risk factors has a favorable effect on the manifestation of arteriosclerotic vascular diseases. The dietetic measures constitute the therapeutic basis of this; atherogenic low-density lipoproteins (LDL) are lowered and at the same time the high-density lipoproteins (HDL) are raised. The lowering of alimentary cholesterol intake and selection of suitable fats with a high percentage of polyunsaturated fatty acids is at the forefront. The diet should be supplemented by roughage and soya proteins. In particular, obese patients with disorders of lipid metabolism should pay attention to the guidelines indicated.

Diet, Reducing↗

PubMed1983

Vanadium inhibits oxidative drug demethylation in vivo in mice.

Sodium vanadate inhibits the oxidative demethylation of substrates of the cytochrome P-450-dependent monooxygenase system in vivo in mice. [14C]Methacetin and 7-[methoxy-14C] coumarin were used as substrates, and the exhaled 14CO2 was monitored using the technique of the breath test. The inhibition is of short duration and begins to subside after about 10 min. The inhibition is dose-dependent; half-maximal effect is achieved at a dose of approximately 60 mumol/kg. The inhibition pattern is identical for both substrates, although 62% of the label of [14C] methacetin and only 10% of 7-[methoxy-14C] coumarin are enhaled within 1 h. Pretreatment with ascorbic acid (50 mg/kg p.o.) drastically diminishes the observed inhibitory effect of vanadate. Similarly, application of an equimolar dose of vanadyl sulphate produces a comparatively weak retardation of 14CO2 exhalation. The effect of vanadate is thought to occur by its competition for electrons normally transferred to cytochrome P-450.

Acetamides↗

PubMed1973