Lymphadenectomy as a part of resection for non-small cell lung carcinoma.
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Carcinoma, Non-Small-Cell Lung↗
Biomedical subjects
M Herman
Publications and source records attributed to M Herman.
Extended lung resections after inductive chemotherapy.
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Antineoplastic Agents↗
Expression of stromelysin-3 in atherosclerotic lesions: regulation via CD40-CD40 ligand signaling in vitro and in vivo.
Stromelysin-3 is an unusual matrix metalloproteinase, being released in the active rather than zymogen form and having a distinct substrate specificity, targeting serine proteinase inhibitors (serpins), which regulate cellular functions involved in atherosclerosis. We report here that human atherosclerotic plaques (n = 7) express stromelysin-3 in situ, whereas fatty streaks (n = 5) and normal arterial specimens (n = 5) contain little or no stromelysin-3. Stromelysin-3 mRNA and protein colocalized with endothelial cells, smooth muscle cells, and macrophages within the lesion. In vitro, usual inducers of matrix metalloproteinases such as interleukin-1, interferon-gamma, or tumor necrosis factor alpha did not augment stromelysin-3 in vascular wall cells. However, T cell-derived as well as recombinant CD40 ligand (CD40L, CD154), an inflammatory mediator recently localized in atheroma, induced de novo synthesis of stromelysin-3. In addition, stromelysin-3 mRNA and protein colocalized with CD40L and CD40 within atheroma. In accordance with the in situ and in vitro data obtained with human material, interruption of the CD40-CD40L signaling pathway in low density lipoprotein receptor-deficient hyperlipidemic mice substantially decreased expression of the enzyme within atherosclerotic plaques. These observations establish the expression of the unusual matrix metalloproteinase stromelysin-3 in human atherosclerotic lesions and implicate CD40-CD40L signaling in its regulation, thus providing a possible new pathway that triggers complications within atherosclerotic lesions.
Animals↗
High-Resolution FT Spectroscopic Investigation of Acetaldehyde around 7 µm.
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Journal Article↗
Rotation-Vibration Constants for the nu1, nu22, nu24, nu22 + nu24, and Ground States in Pyrrole (12C4H5N).
We have recorded the infrared absorption spectrum of pyrrole at 0.005 cm-1 spectral resolution using a Fourier transform interferometer. The rotational analysis of the symmetric out-of-plane C-H bend 22(1)0 fundamental band at 722.132993(5) cm-1 was performed, allowing 6760 lines to be assigned. These lines were fitted simultaneously to literature data on nu1 [A. Mellouki, R. Georges, M. Herman, D. L. Snavely, and S. Leytner, Chem. Phys. 220, 311-322 (1997)] and microwave lines [G. Wlodarczak, L. Martinache, J. Demaison, and B. P. Van Eijck, J. Mol. Spectrosc. 127, 200-208 (1988)]. A set of rotation parameters was determined for the ground state in Ir and IIIr representations, together with vibration-rotation constants for the v1 = 1 and v22 = 1 vibrational states. The fine structure in the strongest of the hot bands in that range was highlighted by division, from the experimental data, of the spectrum of the 22(1)0 band, computed using the vibration-rotation parameters. The rotational assignment of 930 lines in the strongest hot band was performed. The 22(1)024(1)1 vibrational assignment is proposed, leading to x22,24 = 1.90 cm-1. The transition dipole matrix element for the 22(1)0 band is estimated to || || = 2 x 10(-4) D. Copyright 1999 Academic Press.
Journal Article↗
Effective Rotation-Vibration Parameters for the nu8 and nu4 + nu12 Bands of Ethane.
The jet-cooled spectrum of ethane in the region of the nu8 perpendicular fundamental vibration at 1472 cm-1 was recorded with a Fourier transform spectrometer at 0.01 cm-1 instrumental resolution. About 1150 lines were assigned to 18 subbands of nu8 ranging from K"DeltaK = -9 to +8. About 160 lines were assigned to three subbands (K"DeltaK = 0 to +2) of the nu4 + nu12 combination band near 1481 cm-1. Torsional splittings were observed for the majority of the transitions. A stack-by-stack analysis was performed, resulting in effective rovibrational parameters for the upper states nu8 and nu4 + nu12. A number of perturbations was observed and is discussed. Copyright 1999 Academic Press.
Journal Article↗
Weak Combination Bands in the 3-µm Region of Ethane.
The infrared spectrum of ethane in the region of the CH-stretching fundamental vibrations, between 2600 and 3300 cm(-1), was recorded with a Bruker IFS120HR Fourier transform spectrometer. Jet-cooled and room-temperature conditions were used at 0.01 and 0.005 cm(-1) instrumental resolution, respectively. Ten weak combination bands were observed and vibrationally assigned, in addition to the three principal bands in the region (nu(5), nu(7), and nu(8) + nu(11) ( parallel component)). A stack-by-stack analysis was performed for five of the combination bands. Their origins were determined as follows: nu(8) + nu(12) ( parallel component) at 2665.1512(30) cm(-1), nu(2) + nu(6) at 2753.326(32) cm(-1), nu(6) + nu(11) at 2844.13069(75) cm(-1), nu(8) + nu(11) ( perpendicular component) at 2930.705(90) cm(-1), and nu(4) + nu(10) at 3255.568(50) cm(-1). Effective rovibrational parameters were obtained for the corresponding upper states. New information was also obtained on the five remaining combination bands. Their approximate origins are: nu(8) + nu(12) ( perpendicular component) at 2660 cm(-1), nu(2) + nu(4) + nu(12) at 2860 cm(-1), nu(4) + 2nu(9) + nu(12) at 3090 cm(-1), 3nu(4) + nu(8) + nu(9) at 3157 cm(-1), and nu(4) + 2nu(8) at 3225 cm(-1). Copyright 1999 Academic Press.
Journal Article↗
Rotational Analysis of the nu(7) Band in Furan (C(4)H(4)O).
We recorded and analyzed the absorption spectrum of the nu(7) fundamental band in furan, observed around 995 cm(-1). Fourier transform (FT) spectroscopy was used at ULB to record the spectrum under room-temperature conditions, at 0.01 cm(-1) instrumental resolution. Diode-laser (DL) spectroscopy in a supersonic jet was used to record some portions of the band at Lille, revealing the fine structure around the band center. Pure rotation (MMW) data in the upper state were also recorded at Lille. Some 5559 FT, 101 DL, and 23 MMW data were assigned in this work. We fitted, on one hand, the MMW and DL data together and, on the other hand, the MMW, DL, and FT data simultaneously using a weighted procedure, constraining the ground state constants to their value determined from the microwave data in the literature. The results from these fits are provided and the constants discussed. Ab initio calculations are also performed to provide a force field which is used to support the very strong increase with the vibrational excitation observed in the inertial defect determined from the experimental rotational constants. Copyright 1999 Academic Press.
Journal Article↗
Calcium balance during pulse alfacalcidol therapy for secondary hyperparathyroidism in CAPD patients treated with 1.0 and 1.25 mmol/L dialysate calcium.
Hypercalcemia frequently occurs in continuous ambulatory peritoneal dialysis (CAPD) patients treated with calcium carbonate and vitamin D metabolites. To reduce the incidence of this complication, it has been proposed to use dialysate solutions with a low calcium concentration. However, there is concern that these solutions may lead to a negative calcium balance. We measured calcium balance in 13 CAPD patients with secondary hyperparathyroidism who were treated with calcium carbonate and alfacalcidol, 2 microg twice weekly, while using 1.0- (1.0 group) and 1.25-mmol/L (1.25 group) dialysate calcium solutions. Calcium absorption was measured after the administration of Ca47. Results for the 1.0 (n = 6) and 1.25 (n = 7) groups included fractional calcium absorptions of 0.14 (range, 0.09 to 0.27) and 0.08 (range, 0.03 to 0.40; P = not significant [NS]) and calcium absorptions of 380 +/- 92 and 331 +/- 83 mg/d (P = NS). Dialysate calcium losses were 93 +/- 20 and 91 +/- 26 mg/d, and total calcium losses (dialysate and urine) were 106 +/- 16 and 108 +/- 40 mg/d (P = NS). Calcium balance was positive in all patients (274 +/- 92 and 223 +/- 65 mg/d; P = NS). These data suggest that the use of 1.0- and 1.25-mmol/L calcium solutions in conjunction with calcium carbonate and pulse alfacalcidol therapy is associated with a positive calcium balance in CAPD patients.
Absorption↗
Effects of antiprogestins on the rate of proliferation of breast cancer cells.
We have examined the influence of progestins (progesterone, R5020) and antiprogestins (RU486, ZK98299, Org 31710 and Org 31806) on the rate of proliferation of wild type T47D cells cultured in whole fetal bovine serum (FBS) or in single charcoal stripped fetal bovine serum (SSFBS). All of the progesterone antagonists RU486, ZK98299 and two novel antiprogestins Org 31710 and Org 31806 inhibited cell proliferation when cells were cultured in FBS. In contrast, all of the antiprogestins with the exception of ZK98299 enhanced cell growth when cells were cultured in SSFBS. This stimulatory effect of RU486 was observed only at a high concentration of the ligand (1 microM). The effect of R5020, however, was concentration independent. The number of cells in the presence of RU486 was approximately 600% followed by R5020 approximately 400% above control values after a 28 day culturing period. In contrast, when the cells were grown in the presence of medium containing non-stripped whole serum, RU486 inhibited the extent of cell proliferation by 45%. Estradiol (E2) stimulated the rate of proliferation in cells cultured in SSFBS. Similar to when cells were cultured in whole serum, the antiprogestins inhibited cell growth in E2-supplemented SSFBS. Detection of the growth enhancement effects of progesterone receptor (PR) ligands such as RU486 and R5020 on the cells grown in charcoal-stripped medium appear to require the removal of E2 by charcoal stripping of the serum.
Blood↗
Current concepts in gastrointestinal photodynamic therapy.
OBJECTIVE: To review current concepts of photodynamic therapy (PDT) applied to the treatment of tumors of the gastrointestinal tract. SUMMARY BACKGROUND DATA: PDT initially involves the uptake or production of a photosensitive compound by tumor cells. Subsequent activation of the photoreactive compound by a specific wavelength of light results in cell death, either directly or as a result of vascular compromise and/or apoptosis. METHODS: The authors selectively review current concepts relating to photosensitization, photoactivation, time of PDT application, tissue selectivity, sites of photodynamic action, PDT effects on normal tissue, limitations of PDT, toxicity of photosensitizers, application of principles of PDT to tumor detection, and current applications of PDT to tumors of the gastrointestinal tract. RESULTS: PDT is clearly effective for small cancers, but it is not yet clear in which cases such treatment is more effective than other currently acceptable approaches. The major side effect of PDT is cutaneous photosensitization. The major limitation of PDT is depth of tumor kill. As data from current and future clinical trials become available, a clearer perspective of where PDT fits in the treatment of cancers will be gained. Many issues regarding pharmacokinetic data of photosensitizers, newer technology involved in light sources, optimal treatment regimens that take advantage of the pharmacophysiology of photoablation, and light dosimetry still require solution. One can foresee application of differing sensitizers and light sources depending on the specific clinical situation. As technologic advances occur, interstitial PDT may have significant application. CONCLUSIONS: PDT has a potentially important role either as a primary or adjuvant mode of treatment of tumors of the gastrointestinal tract.
Gastrointestinal Neoplasms↗
Our experience with 3D CT.
The authors present the results of 9-year work with 3D CT in orthopaedics and musculoskeletal traumatology. In the years 1990-1998, the method was used in the examination of 59 patients (27 males, 20 females, and 12 children). The place of 3D CT in clarifying areas of complex musculoskeletal anatomy for operative treatment is discussed and presented as a contribution to the indication conference. According to the authors' experience, in many cases 3D CT increases the quality of diagnostics. Most of all, it helps the surgeon in operation planning. 3D reconstructions are particularly valuable in proximal humerus fractures, spine fractures and tumours, acetabulum fractures, as well as in conditions after hip dysplasia, LCPD and coxa vara adolescent.
Adult↗
High-Resolution FTIR Spectroscopy Using a Jet: Sampling the Rovibrational Spectrum of 12CH4
We have recorded high-resolution absorption spectra of 12CH4 cooled in a jet, using a Fourier transform interferometer. The expected nuclear spin symmetry conservation in the jet is exhibited from data recorded around 3000 cm-1 showing the relative intensity of the lines starting from the J" = 2 para and ortho levels. Spectra recorded around 4300 cm-1 are used to validate existing rovibrational parameters. Spectra recorded around 6000 cm-1 allow new rovibrational assignments to be performed in the N = 4 polyad. In particular, lines in the nu1 + nu3 band are identified for the first time. Copyright 1998 Academic Press. Copyright 1998Academic Press
Journal Article↗
High-Resolution Jet-Cooled Spectroscopy of SF6: The nu2 + nu6 Combination Band of 32SF6 and the nu3 Band of the Rare Isotopomers.
The Fourier transform infrared spectrum of SF6 was recorded in a supersonic expansion jet of an SF6/argon mixture. The SF6:Ar seeding ratio was 2:3. The instrumental bandwidth was 0.005 cm-1. A globar source and an MCT detector were used. A rotational temperature of approximately 30 K was achieved. The nu2 + nu6 combination band of 32SF6 was analyzed using a modified version of the spherical top data system (STDS) programs developed in Dijon. A very good fit was obtained for this band with an rms of 0.0036 cm-1. The effective Hamiltonian was developed up to fourth order for the nu2 + nu6 part, to second order for the nu2 and ground state parts, and to first order for the nu6 part. Five hundred twenty-one transitions were assigned, 40 of them reaching the F2u forbidden sublevel. The positions of the two F1u and F2u sublevels are found to be 991.276 and 989.487 cm-1, respectively. The nu3 band of the 33SF6 and 34SF6 isotopomers were also analyzed. Parameters and simulations are presented. A first detection of the nu3 Q branch of 36SF6 (0.02% natural abundance) is reported. Copyright 1998 Academic Press.
Journal Article↗
Postoperative rhabdomyolysis in patients with end-stage renal failure.
Four patients with end-stage renal failure on intermittent hemodialysis in whom rhabdomyolysis developed after major surgery are described. This possibly underdiagnosed complication was manifested by extreme hyperphosphatemia, hypocalcemia, and elevated creatine phosphokinase levels. Serum myoglobin levels further supported the diagnosis. The metabolic abnormalities reached a peak on the fourth postoperative day. The possible precipitating factors included opiates used for anesthesia and postoperative pain control, anesthetic agents, and surgical position. The preferred treatment option is increasing dialysis to control hyperphosphatemia and hypocalcemia.
Adult↗
A modified method of intracytoplasmic sperm injection without the use of polyvinylpyrrolidone.
In a controlled study we compared the outcome of intracytoplasmic sperm injection (ICSI) performed by two different methods. The oocytes from 20 patients were equally divided into two groups and injected either by conventional ICSI using polyvinylpyrrolidone (PVP) or by a modified PVP-free ICSI procedure. While in the conventional ICSI method the spermatozoon is aspirated into the injection pipette, in the modified ICSI procedure the spermatozoon is attached to the end of the narrow micropipette by aspirating its tail. The sperm head is never drawn into the pipette. Accordingly, even a fast-moving spermatozoon can be 'caught' easily. As a result of such an aspiration the spermatozoon loses its motility. Therefore, PVP is required neither to slow down the movement of the spermatozoon nor to facilitate the movement of the solution in the injection pipette. A total of 230 mature oocytes were injected by both methods and the results were analysed. No differences were observed in survival rate between the two ICSI procedures (89% and 91%, respectively). However, the proportion of normally fertilized oocytes was significantly higher after microfertilization by modified ICSI (74%) when compared with the outcome of the conventional ICSI method (62%). The frequency of abnormal fertilization was not influenced by the method of ICSI used. The cleavage rate and quality of resulting embryos were also comparable. In conclusion, we have demonstrated a modified ICSI method which does not require the use of PVP. When compared with the conventional ICSI procedure, even better fertilization rates can be achieved. The proposed ICSI modification may provide an alternative procedure for elimination of the potentially harmful effects which may be associated with conventional ICSI.
Cleavage Stage, Ovum↗
The femoral artery-femoral vein polytetrafluoroethylene graft: a 14-year retrospective study.
BACKGROUND: The use of the femoral vessels for permanent haemodialysis access has been neglected during the last two decades. Since 1981 femoral artery-vein loop polytetrafluoroethylene grafts have been constructed in our chronic haemodialysis patients. This study examines results obtained in patients with this particular graft over the last 14 years. METHODS: This clinical study is retrospective in nature. Overall 35 patients, with 37 femoral grafts, are included. Inclusion and exclusion criteria for this type of graft are given and the surgical procedure detailed. RESULTS: Seven patients had femoral grafts used as primary dialysis access. Twenty-eight patients had femoral grafts used after multiple access failures. There was no perioperative mortality. Immediate thrombotic non-function of the graft occurred in three patients. In the long term no patient death was related to the femoral grafts. Twenty-seven (73%) grafts had no long-term complications. The leading cause for graft 'loss' was patient death; in the first year 10 grafts were lost, eight because of patient death. All eight patients died with functioning grafts. Median graft survival was 21 months in all patients and 28 months in non-diabetic patients. Twenty-seven (73%) grafts were patent at the end of the first year, 33% of grafts were still patent after 5 years. Worsening claudication occurred in four patients; one diabetic required foot amputation. Four patients had late graft thrombosis; only two patients had bacteraemia originating from the femoral graft. Urea reduction ratio greater than 60% was measured in 87.5% of patients. CONCLUSION: The femoral artery vein graft is a good primary and secondary haemodialysis access. Both infection and thrombosis rates are low and graft survival is comparable, if not superior to, that of upper-limb grafts. The graft is easy to cannulate, can be used early, is easily protected, and is cosmetically acceptable.
Adult↗