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Biomedical subjects

M Hille

Publications and source records attributed to M Hille.

At least 19 recordsLinked to original sources
PubMed2002

Photodynamic therapy mediated induction of accelerated re-endothelialisation following injury to the arterial wall: implications for the prevention of postinterventional restenosis.

OBJECTIVE: Accelerated re-endothelialisation may inhibit the development of restenosis. Basic Fibroblast Growth Factor (bFGF) plays a key role for early proliferative activity in the artery following injury. Therefore, this study was devised to examine the effect of photodynamic therapy (PDT) on post-injury re-endothelialisation in vivo, and bFGF-mRNA expression in endothelial cells (EC) in vitro. MATERIALS AND METHODS: Rat carotid arteries were balloon-injured prior to PDT. Arteries were analysed after 1, 3, 5, 14 and 30 days. Morphometric measurements were undertaken following injection of 0.5% Evans Blue which stains non-endothelialised surfaces only. To identify EC, immunohistochemistry (CD-31) was performed. Proliferation was assessed by fluorescence cell counting. PCR quantification of bFGF-mRNA expression and proliferation were assessed in bovine aortic EC which were plated on isolated, PDT-treated EC-derived extracellular matrix at (12), 24, 48 (72 h). RESULTS: Three days following PDT, arteries displayed significantly increased endothelial lining (p = 0.02), which was more pronounced at 5 (p = 0.03) and 14 days (p = 0.02). At 30 days no relevant differences between PDT and control were noted. EC proliferation on PDT-treated matrix was significantly increased at 24, 48, and 72 h (p = 0.0004), whereas bFGF-mRNA expression was significantly increased at 24 h only (p = 0.007). CONCLUSION: Post-injury PDT appears to accelerate re-endothelialisation. Expression of bFGF-mRNA, however, although increased shortly after PDT, may not be responsible for a constant stimulation of EC proliferation.

Animals↗
PubMed2001

Dual role of GdmH in producer immunity and secretion of the Staphylococcal lantibiotics gallidermin and epidermin.

The biosynthetic gene clusters of the staphylococcal lantibiotics epidermin and gallidermin are distinguished by the presence of the unique genes epiH and gdmH, respectively. They encode accessory factors for the ATP-binding cassette transporters that mediate secretion of the antimicrobial peptides. Here, we show that gdmH also contributes to immunity to gallidermin but not to nisin. gdmH alone affected susceptibility to gallidermin only moderately, but it led to a multiplication of the immunity level mediated by the FEG immunity genes when cloned together with the gdmT gene, suggesting a synergistic activity of the H and FEG systems. gdmH-related genes were identified in the genomes of several bacteria, indicating an involvement in further cellular functions.

Amino Acid Sequence↗
PubMed1997

Secretion of the lantibiotics epidermin and gallidermin: sequence analysis of the genes gdmT and gdmH, their influence on epidermin production and their regulation by EpiQ.

The closely related lantibiotics epidermin and gallidermin are produced by Staphylococcus epidermidis Tu3298 and S. gallinarum Tu3928, respectively. The epidermin biosynthetic genes involved in maturation, regulation, and immunity have been identified previously. How epidermin or gallidermin is secreted, however, has remained unclear. Here, we characterize two additional genes, epiH and epiT, as well as the homologous gallidermin genes gdmH and gdmT. EpiT and GdmT are similar to one-component ABC transporters that are involved in the secretion of proteins or peptides. EpiH and GdmH are hydrophobic proteins without conspicuous similarities to other proteins. Comparison of the gene sequences revealed that epiT is incomplete, having an internal deletion that causes a frame shift and a second deletion at the 3'-end, while gdmT is intact. Introduction of epiT and epiH into the heterologous host S. carnosus (pTepi14) bearing the maturation and regulation genes had no significant effect on the rather low level of epidermin production. The presence of the homologous gdmT and gdmH, however, resulted in a strong increase (seven- to tenfold) in the production level, which is very likely to be due to increased efficiency of epidermin secretion. Both gdmT and gdmH were necessary for this effect, indicating that the two gene products cooperate in some way. In the epidermin-producing wild-type strain Tu3298, which contains epiH and the disrupted epiT, the addition of gdmT alone led to a two-fold increase in epidermin production. Both gdmT and gdmH and the corresponding epi genes were activated by the transcriptional regulator EpiQ; this is in accordance with the presence of putative EpiQ operator sites in the promoter regions.

Amino Acid Sequence↗
PubMed1996

A dominant inhibitory mutant of the type II transforming growth factor beta receptor in the malignant progression of a cutaneous T-cell lymphoma.

In many cancers, inactivating mutations in both alleles of the transforming growth factor beta (TGF-beta) type 11 receptor (TbetaRII) gene occur and correlate with loss of sensitivity to TGF-beta. Here we describe a novel mechanism for loss of sensitivity to growth inhibition by TGF-beta in tumor development. Mac-1 cells, isolated from the blood of a patient with an indolent form of cutaneous T-cell lymphoma, express wild-type TbetaRII and are sensitive to TGF-beta. Mac-2A cells, clonally related to Mac-1 and isolated from a skin nodule of the same patient at a later, clinically aggressive stage of lymphoma, are resistant to TGF-beta. They express both the wild-type TbetaRII and a receptor with a single point mutation (Asp-404-Gly [D404G]) in the kinase domain (D404G-->TbetaRII); no TbetaRI or TbetaRII is found on the plasma membrane, suggesting that D404G-TbetaRII dominantly inhibits the function of the wild-type receptor by inhibiting its appearance on the plasma membrane. Indeed, inducible expression, under control of a tetracycline-regulated promoter, of D404G-TbetaRII in TGF-beta- sensitive Mac-1 cells as well as in Hep3B hepatoma cells results in resistance to TGF-beta and disappearance of cell surface TbetaRI and TbetaRII. Overexpression of wild-type TbetaRII in Mac-2A cells restores cell surface TbetaRI and TbetaRH and sensitivity to TGF-beta. The ability of the D404G-TbetaRH to dominantly inhibit function of wild-type TGF-beta receptors represents a new mechanism for loss of sensitivity to the growth-inhibitory functions of TGF-beta in tumor development.

Amino Acid Sequence↗
PubMed1989

[Carcinoid tumor with cystic liver metastases].

Generally metastases of carcinoid tumours are pseudocystic in nearly 10% of the patients. In our case the guiding diagnostic importance of sonography and computed tomography could be proved. Results have to be completed by histologic findings and hormonal analysis (serotonin, 5-hydroxyindol acetic acid). - Morphological signs especially of the new imaging techniques are often a supposition for the modern therapy, e.g. the application of serotonin antagonists, local perfusion of cytostatic substances and if possible transplantation of the liver.

Angiography↗
PubMed1988

[Report of experiences in 20 years use of intrauterine fetal transfusion in severe Rh-induced hemolytic disease of newborn].

136 intrauterine transfusions in 90 fetuses between the 25th and 34th gestational week with severe hemolytic disease because of Rh-incompatibility have been performed after a prenatal step by step diagnostic in the years 1967 till 1986. The reason of the immunization was mistransfusion or omitted immune prophylaxis in 25 cases. 25 from 67 live born children died. There were 23 still births. 34 from 43 fetuses without hydrops survived, but only 8 from 47 fetuses with hydrops. The survival rate over all was 46.7 per cent.

Blood Transfusion, Intrauterine↗
PubMed1986

[The fight against hemolytic disease of the newborn-- an example of interdisciplinary cooperation].

The practical proceeding used in checking anti-D-immunoprophylaxis, in monitoring carriers of antibodies during gravidity and in treating newborns is represented. Details of patients selecting by means of step by step prenatal diagnostics as well as technique and results of intrauterine transfusion are described. After the general introduction of anti-D-prophylaxis, the morbidity resulting from morbus haemolyticus could be reduced by 80% approximately. The survival rate of foeti intrauterinely transfused amounts to 49%.

Blood Transfusion, Intrauterine↗
PubMed1984

[Clinical relevance of the so-called arteria lusoria in childhood].

Although arteria lusoria is relatively frequent, symptomatic cases are rare in childhood. Symptoms are not caused by the retroesophageal course of the vessel itself, but by additional anomalies of the other branches of the aortic arch, above all by the pretracheal course of the equilateral arteria carotis communis. In very rare cases, the anomalous right arteria subclavia passes between esophagus and trachea and may cause threatened impending respiratory disturbances together with an aberrant course of the arteria carotis. The diagnostic and therapeutic procedure is discussed; the development of pulmonary hypertension in vascular respiratory disturbances is pointed out.

Aorta, Thoracic↗
PubMed1980

[Experience obtained from intra-uterine foetal transfusion to cope with severe rhesus-caused haemolytic disease of foetus - ten-year report (author's transl)].

Sixty-three intra-uterine transfusions were applied to 44 foetuses with foetohaemolytic disease, in Rostock, between 1967 and 1976. Patients were selected for treatment by means of stepwise prenatal diagnosis. Details of that selection are described together with the technique of intra-uterine transfusion, with reference being made also to risks and complications. The survival rate of children, following intra-uterine transfusion, accounted for 41 per cent. The survival rate of non-hydropic foetuses was 76 per cent and thus much beyond that of hydropic foetuses of whom only 19 per cent survived. While the incidence of severe foetohaemolytic disease due to blood-group incompatibility is on a declining trend, intra-uterine foetal transfusion should be maintained as a possible therapy for certain cases.

Amniotic Fluid↗
PubMed1978

[Compression tests on the human hip joint (author's transl)].

Three human pelvises were subjected to static loading in the course of the experiments. The values determined for the elasticity modules lie between 2 and 4.5 kp/min2. The values range between 5 and 14 kp/mm2 if only the compacta is considered. The experiments show that the elasticity modules obtained at cross sections through the Os coxae are higher than those for sections through the hip joint and its immediate surroundings. Here the elasticity module reaches the minimum value of 0.77 kp/cm2 (Fig. 1, cross section No.2, Table 3). The highest breaking strain was 1045 kp (Pelvis II). The longitudinal contraction of the three pelvis was 26.6 mm on average. For the mean height of 27.2 cm for the specimens used, this means that the pelvis, as a system (with hip joint) can be reduced in length by about 10% before a fracture occurs under static load conditions.

Biomechanical Phenomena↗