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Biomedical subjects

M Hirono

Publications and source records attributed to M Hirono.

At least 73 records · Page 4Linked to original sources
PubMed1985

[Phase II study of 4'-O-tetrahydropyranyl-adriamycin (THP-ADM) in patients with gynecological cancer].

We conducted a joint phase II study in 76 patients with gynecological cancer (42 patients with ovarian cancer, 22 patients with cervical cancer, 10 patients with endometrial cancer and 2 patients with vaginal cancer). The response rate was 25.0% in the patients with ovarian cancer, 13.3% in those with cervical cancer, and 28.6% in those with endometrial cancer. The overall response rate was 23.1%. When the patients were classified according to dose schedules, the highest response rate was obtained in the group administered THP-ADM at a dose of 60 mg per body by single i.v. injection at 3-week intervals. Such side effects as myelosuppression and gastrointestinal disturbances were observed, but alopecia, a marked side-effect of ADM administration, was mild, and no cardiac toxicity was seen in any of the patients.

Adult↗
PubMed1984

Early gastric cancer with widespread lymph node metastases; a case report.

The resected stomach from a 58-year old male patient showed a 4.3 X 3.2 cm, depressed (II c) lesion in the body, histology of which revealed moderately differentiated tubular adenocarcinoma with invasion reaching the submucosa. Widespread metastases of lymph nodes including superior mesenteric and para-aortic ones were noted. The patient once worked in a mustard gas (Yperite) factory, and had chronic bronchitis. The exposure to this toxic substance was discussed in relation to the diffuse metastases of cancer.

Adenocarcinoma↗
PubMed1984

[Surgical treatment of Borrmann type 4 (diffuse) gastric cancer].

A total of 435 cases with stage II-IV gastric cancer resected in our department was investigated. Cases with Borrmann 4-type cancer showed the poorest prognosis in all stages compared with other types of cancer. None of three drugs-PS-K, FT-207 and Levamisole-was specifically effective on Borrmann 4-type cancer in two randomized controlled trials. A study of in-vitro lymphocyte responsiveness to mitogens suggested the presence of a suppressive factor in the serum of the patient with poorly differentiated cancer. An immunofluorescent study using monoclonal antibodies against large and small intestinal mucin antigens showed strong positivity in poorly differentiated cancers.

Female↗
PubMed1984

[Sensitivity test of anticancer agents using human tumor xenografts in nude mice with special reference on correlation between clinical effects and chemotherapy].

In order to improve the response rate of chemotherapy, we have investigated to the method for rapid and accurate prediction of the most appropriate anticancer agents. Thus, a rapid method using nude mice has been established as in vivo model for assessing chemosensitivity of individual human tumors, in which the final evaluation was made with 3H-thymidine incorporation and histological changes of tumor cells treated. Correlation between the sensitivity of anticancer agents and retrospective results of gastrectomy in stage IV gastric cancer was investigated. Our sensitivity test indicated a good correlation with clinical therapeutic and immunological effects. From these results, it seems reasonable to conclude that the sensitivity test using human tumor/nude mouse system may be useful for selection of appropriate agents to treat patients with cancer.

Animals↗
PubMed1984

[Four gastrectomized cases associated with chronic leukemia].

We reviewed 4 gastrectomized cases associated with chronic leukemia. Three patients had chronic myelogenous leukemia, one had hairy cell leukemia. Subtotal gastrectomy was performed in all cases; two operations were for gastric cancer and two for bleeding gastric ulcers. The spleen was removed in three patients without any trouble. There were no difficulties in managing the patients during and after surgery and their leukemia was not aggravated during these periods.

Aged↗
PubMed1984

[A case of minute carcinoma associated with an atypical epithelial lesion of the stomach and histopathological analysis].

A patient with minute carcinoma associated with an atypical epithelial lesion (ATP) of the stomach is reported. The resected stomach from this 81-year-old male exhibited a 1.3 X 1.0 cm elevated ATP; microscopic examination revealed a minute (0.4 mm) carcinoma. The lesion was differentiated tubular adenocarcinoma and was suggestive of one-gland carcinoma. It consisted of one faveola in the upper half and multibranched , compound alveolar glands in the lower half, structurally mimicking the initial stage of intestinal metaplasia. The lesion existed in small metaplastic foci; the surface foveolar epithelia of the lesion showed no cancerous changes. These findings suggested that this minute carcinoma occurred in the initial stage of intestinal metaplasia.

Adenocarcinoma↗
PubMed1983

Clinico-pathological studies on ovarian metastasis from gastric cancer.

Thirty autopsied female patients with gastric cancer were investigated clinico-pathologically, regarding ovarian metastases. According to the histological pattern of the ovarian metastases, there were five types; no-metastasis in five, capsular type (cancer involvement confined to the capsule) in three, hilar type (carcinoma is evident in the hilus but not in the cortex or medulla) in four, multifocal type (multifocal growth of cancer in the cortex and/or medulla) in eight and diffuse type (diffuse proliferation of cancer) in ten. Although marked peritoneal dissemination was noted in 27, the capsule of the ovaries was usually intact. These results suggest that in the development of ovarian metastasis, cancer cells first reach the hilus, then spread into the medulla or cortex multifocally, and later grow diffusely. While cancer cells reach and cover the capsule of the ovary in many instances, they seldom break and invade it. All seven patients, except one unknown patient, with no-metastasis and with the capsular type were postmenopausal, while one hilar, two multifocal and five diffuse type women were premenopausal. This indicates an "affinity" of ovarian tissue for cancer cells, in younger patients.

Adenocarcinoma↗
PubMed1983

Involvement of Tetrahymena intermediate filament protein, a 49K protein, in the oral morphogenesis.

To study the biological function of Tetrahymena intermediate-type filament protein (a 49K protein), we examined the immunofluorescence localization of 49K protein within Tetrahymena cells. The results showed that the immunofluorescence was localized in the oral apparatus, mitochondria and mucocysts. Among them, the fluorescence in the oral apparatus was of high interest in its unique region and vicissitude in the cell cycle: a tau-shaped region of the oral apparatus intensely fluoresced during interphase, but the fluorescence completely disappeared during dividing phase. The tau-shaped region corresponded to 'posterior connectives' and the root part of 'deep fiber', to the conjunction parts of microtubule bundles. In the those parts, there was electron-dense material in the microtubule bundles. Hence, it is conceivable that 49K protein corresponds to the dense material and has a function of microtubule bundle conjunction. On the other hand, disappearance of immunofluorescence from the old oral apparatus of most dividing cells reflected the oral apparatus regression and remodelling which have been known as necessary sequential events in the cell cycle. We observed that oral fluorescence disappeared concurrently with the onset of oral regression and of constriction of division furrow, whereas at a late dividing stage immunofluorescence began to appear simultaneously in both new and old oral apparatus. Thus, the 49K protein may play a crucial role(s) not only in the morphogenesis of oral primordia but also in the transient morphogenesis in the old oral system.

Animals↗
PubMed1983

[Evaluation of chemotherapy for resected stomach cancer: second-look operation].

During the period from Jun. 1973 to Dec. 1981, seven hundred and three patients with primary gastric cancer have undergone gastrectomy at the Surgical Department, Research Institute for Nuclear Medicine & Biology, Hiroshima University. As adjuvant immunochemotherapy, large dose of Mitomycin C was routinely administered; 20 mg on the day of gasrectomy and additional 10 mg on the next day, intravenously. Furthermore, PS-K, a protein-bound polysaccharide, and/or Tegafur were administered for a prolonged period of time. Two hundred and forty of 703 patients were non-curatively resected cases. The factors by which gastrectomy was limited to non-curative resection at the primary gastrectomy were peritoneal dissemintions, liver metastases or invasions to the contiguous organs. Of the 244 patients, 17 were re-laparotomized and at the same time the evaluation of the chemotherapeutic effects was investigated. Of these 17, six patients were administered with large dose OK-432, preparation of Bac. streptococcus hemolyticus, intratumorally or intraperitoneally. In three patients, Intraabdominal patching with Adriamycin (ADM) was performed the direct cytocidal activities and strong stickiness of this drug to the malignant cells. From the findings of relaparotomy, it was suggested that large dose OK-432 administration or ADM patching was effective for peritoneal disseminations.

Adult↗
PubMed1983

Establishment of different clonal strains from a human sarcoma of the stomach: tumorigenic heterogeneity in athymic nude mice.

Twenty five clonal strains have been isolated from a single human sarcoma of the stomach. Two different types of clones have been recognized by their morphology and behavior in vitro. Type I clones were characterized by criss-crossed arrays and multilayers with high terminal density. Type II clones grew in a well-organized monolayer with lower saturation density. Although both types of clones exhibited fibroblastic appearance, type I clones showed a more rounded, refractile shape. The cells of this type showed multiple regions of criss-crossed arrays and multilayers throughout the culture vessels. Saturation density of this type of clone was 2- to 3-fold (1.7 to 2.1 X 10(5) cells/sq cm) higher than that of type II clones. Chromosomal analysis revealed that type I clones were human aneuploid ones with modal chromosome numbers ranging from 51 to 61. With the exception of clones 11 and 19, type I clones were able to produce tumors in athymic nude mice when injected s.c. Type II clones exhibited a more flattened and elongated appearance. The cells grew in a well-organized monolayer resembling fingerprint whorls. They showed lower saturation density (0.7 to 0.9 X 10(5) cells/sq cm). Chromosomal examinations revealed the clones to be human aneuploid ones with modal numbers from 47 to 54. Tumor formation was not observed in nude mice given injections of this type of cell. Both types of clones did not bear antigens cross-reacting with the antiserum against mouse spleen cells but had surface antigens which were affected by the antibody against HL-60 cells and complement. These results suggested that this human sarcoma was heterologous and that cells with widely different tumorigenic potential preexisted in the parental cell population.

Adult↗
PubMed1983

[Management of rectal and ureteral obstruction following gastrointestinal cancer].

During the period from June 1973 to August 1982, thirteen patients with gastric cancer and seven patients with colorectal cancer received surgical treatment due to the obstruction of the rectum or ureter. Of thirteen gastric cancer patients, 11 underwent the formation of the artificial anus for rectal obstruction. Nephrostomy was performed in 4 gastric cancer patients with ureteral obstruction. Seven patients with colorectal cancer underwent the formation of the artificial anus for rectal obstruction. In the recurrent cancer of the gastrointestinal tract, obstruction of the rectum or ureter mainly occurred due to peritoneal invasion or tumor formation. During laparotomy, OK-432, a Streptococcal preparation, was administered with a large dose of 100 K. E. to the patients with advanced or recurrent cancer foci. The prognosis for those patients was poor, and the main reason for poor survival was the advancement of disease in the cases of rectal or ureteral obstruction.

Colonic Neoplasms↗
PubMed1981

Comparative studies on cytological and histological evaluations of disseminating peritoneal metastasis in gastric cancer.

Macroscopic disseminating peritoneal metastasis of gastric cancer (P) was investigated in relation to its compatibility with microscopic findings (p) in 52 (P1 19, P2 21, P3 12) cases among 240 gastrectomized cases by cytological, histological, clinical and postmortem findings. In total, numbers of the evaluations for yes (P is p), probable (P is probably p), possible (P is possibly p), probably not (P is probably not p) and no (P is not p) were 28 (53.8%), 8 (15.4%), 14 (26.9%), 2 (3.8%) and 0, respectively, These results indicate that macroscopic disseminating peritoneal metastasis is compatible with microscopic findings, in most cases. Although histology is the most contributory factor for the evaluation of peritoneal dissemination, cytology of lavaged saline from the Douglas pouch also is important.

Adult↗
PubMed1976

Effects of microinjection of various prostaglandins into the 3rd ventricle, median eminence and pituitary on plasma LH in rats.

The effects of microinjection of PG'S (PGE1, E2, F2a) into the 3rd ventricle, median eminence (ME) and anterior pituitary on plasma LH in rats were investigated. Blood samples were obtained by jugular puncture before, and 10 and 45 min after the injection of PGS (50 or 100 mug), plasma LH was measured by radioimmunoassay. In the 3rd ventricle microinjection, PGE2 prodiced a significant rise in plasma LH. PGE1 and F2a did not significantly after plasma LH levels. In the median eminence, PGE2 and E1 produced a significant rise in plasma LH. PGF2a did not alter plasma LH levels. In the pituitary, PGE2 and E1 produced a significant rise in plasma LH. PGF2a did not alter plasma LH levels. These observations indicate that PGs act directly on the hypothalamic-pituitary axis, and that particular PG may be involved in the release of particular horomones from the hypothalamus and pituitary.

Animals↗
PubMed1975

Direct action of prostaglandins on the rat pituitary.

The purpose of this study is to ascertain whether or not prostaglandins (PGs) act directly on the pituitary. In the first experiment, the plasma LH levels were sharply decreased 24 hr after the production of hypothalamic lesions in ovariectomized mature rats. Ten min after the iv injection of PGE1, PGE2 or PGF2alpha, the plasma LH levels increased significantly as compared with the vehicle control. Also, the plasma LH levels were elevated following the iv injection of LH-releasing hormone (LH-RH). In the second experiment, after pretreatment of ovariectomized mature rats with estrogen and progesterone, animals received a SC injection of indomethacin, 2 mg in 0.2 ml of gelatin for 3 days. Twenty-four hr after the last injection, 100 ng of LH-RH were injected into the jugular vein. The response of the pituitary to the LH-RH was significantly lower in the indomethacin-treated rats than in the vehicle control rats. In the 3rd experiment, we tested several PGs for stimulatory effects on LH secretion by injecting them into the anterior pituitary. Following direct microinjection of PGE1 or PGE2 (50 or 100 mug/mul) into the rat pituitary, plasma LH was increased at 10 and 45 min after the injection. However, PGF2alpha at both 50 and 100 mug/5mul, failed to increase the plasma LH levels. In the last experiment, the effect of PGE1, PGE2 or PGF2alpha on in vitro rat anterior pituitary LH release was studied. Addition of PGE1, (2 or 20 mug), PGE2 (0.002 or 0.02 mug) or PGF2alpha (200 mug) to the incubation medium produced a significant increase in LH release into the incubation medium. These observations indicate that sufficient dosages of PGs can also stimulate LH secretion by acting directly on the pituitary.

Animals↗