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M Hooton

Publications and source records attributed to M Hooton.

10 recordsLinked to original sources

The significance of the relationship between a discipline and its practice.

In this paper, it is argued that the absence of conceptual consensus across the university and its sectors is largely responsible for the growing gap between the goals of the discipline and its practice. Both discipline and practice are defined, their symbiotic relationship explored, and specific strategies proposed, to enhance the development of the profession. In particular, the need for a shared conceptual framework which would transcend university and service structures to promote an academic practice is examined in addition to the benefits of instituting theoretically based daily rounds and journal club in the practice setting.

Education, Nursing, Baccalaureate

DNA probes for the detection of mycoplasmas in genital specimens.

The utility of whole-genomic DNA probes for the detection of infections by genital mycoplasmas was investigated in 220 men attending a sexually transmitted diseases clinic. In 144 patients, probe results were compared with quantitative culture results. The prevalence of Mycoplasma hominis was 11% by culture, whereas the prevalence of ureaplasmas was 38%. The M. hominis DNA probe detected 9 of 16 M. hominis culture-positive specimens and 2 of 128 culture-negative specimens. The Ureaplasma urealyticum DNA probe detected 36 of 57 U. urealyticum culture-positive specimens and 18 of 87 culture-negative specimens. Most of the probe-negative culture-positive specimens had colony counts of less than 10(3) organisms/ml of specimen. The DNA probe does not require viable organisms, and the probe-positive, culture-negative specimens suggest that false-negative cultures occurred, perhaps due to specimen handling or insensitivity of culture methods for some strains of mycoplasmas.

Bacteriological Techniques

What is nursing?

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Education, Nursing, Baccalaureate