M Hoppert — Page 2 | PubMed Health

PubMed1990

Electron microscopy of native and artificial methylreductase high-molecular-weight complexes in strain Gö 1 and Methanococcus voltae.

The preparation of inside-out vesicles from methanogenic bacteria with protein cell walls was improved with regard to the preservation of structure and localization of membrane-bound proteins. Complexes similar to the methanoreductosome in the methanogenic bacterium Gö 1 were also found attached to the inner aspect of the cytoplasmic membrane of Methanococcus voltae. Methanoreductosomes were purified from crude extracts of Gö 1-cells by affinity chromatography. Under specific conditions at high protein concentrations methyl-CoM-methylreductase molecules isolated from Gö 1-cells could be reassociated to spherical complexes of various sizes, with an appearance similar to that of methanoreductosomes isolated from strain Gö 1.

Euryarchaeota↗

PubMedDate not supplied

[Unusual late complications following total hip endoprosthesis].

A cementless total hip replacement was implanted in a 52-year-old male patient with coxarthrosis. Rapid cranial migration of the cup and ectopic ossification were subsequently observed. Five years after the original operation, revisional arthroplasty had to be performed to replace the cup. At surgery a large amount of granulomatous tissue and the tip of a deep drainage tube were found. Interposed between the polyethylene inlay and the femoral head of the prosthesis, the drain had not been visible on any of the radiographic controls. The X-ray control performed immediately after the first procedure revealed that the drain had been slung around the neck of the prosthesis stem, a procedure preferred by some surgeons. When the drain was removed, the tip must have been caught between the polyethylene inlay and the femoral head of the prosthesis. It is not clear whether the loosening of the cup was ultimately caused by the increased wear due to the presence of the drain tip. However, in light of this finding we no longer sling the drain around the prosthesis shaft, but lay it along the neck of the femoral component in the same direction as it is routed out transcutaneously.

Foreign-Body Migration↗