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M Hyánková

Publications and source records attributed to M Hyánková.

5 recordsLinked to original sources
PubMed1978

Enhancement of the specific cytotoxic reactivity of HLA antigens on lymphocytes stored at +4 degrees C for 24 hours.

The lymphocytes of some patients (especially with malignancies) as well of healthy subjects, which had been isolated on the day of blood withdrawal from the heparinized blood, show negative or slightly positive reaction with some corresponding HLA sera; after 24 h of storing in the refrigerator at +4 degrees C the lymphocytes tend to restore the cytotoxic reactivity of HLA antigens, and thus, a strongly positive reaction can be recorded. The cause of this phenomenon may be ascribed to the anticomplementary effect of the lymphocyte suspension in barbital buffer.

Blood Preservation↗
PubMed1975

[Studies on changes in lymphocyte HL-A antigens during the course of malignant diseases].

The presence of HL-A antigens 1, 2, 5, 7, 8 and 12 on the lymphocytes of 26 patients with blood diseases and malignant tumours was examined by means of the two-step microcytotoxicity test. The studies carried out several times in the course of the disease and with 4 to 5 sera of the same specificity. Two types of the serological modifications were found: 1. Transient loss of HL-A antigens in 6 patients, 2. Evidence of the polyreactivity of lymphocytes in 3 patients. The polyreactivity was later changed to the loss of HL-A antigens. In one patient, the destruction of lymphocytes in the course of the testing was proved. The importance of the results is discussed. The additional serological, morphological and clinical investigations seems to be necessary.

Antibody Specificity↗
PubMed1975

[The effect of heating and drugs on HL-A antigens and their reactions with cytotoxins].

In some instances the heating of lymphocytes for 2 to 3 minutes at 56 degrees C enhances HL-A antigens or makes it possible to detect these antigens by a twostage microlymphocytotoxic test on preheated lymphocytes only. Similar phenomenons are observed in some tannin (1: 40 000) or 0.1% phenol-treated lymphocytes and after the addition of these solutions during the first stage of the lymphocytotoxic test. Prolonged heating at 56 degrees C leads to nonspecific polyreactivity of lymphocytes, giving false results with all sera. For similar reasons also higher concentrations of tannin and phenol solutions were found dissatisfactory for the pretreatment of lymphocytes. The pretreatment of lymphocytes with 36-0.5% formaline induces full inhibition of specific cytotoxic reactivity of HL-A antigens and their absorption ability with regards to respective HL-A sera. The addition of formaline at 0.06-0.3% concentration during the first and second stages of the test and at the end of the second stage (or simultaneously with eosine) gives also negative results of the cytotoxic test owing to the inhibitory effect of formaline on rabbit complement and HL-A antigens.

Antigen-Antibody Reactions↗