Biomedical subjects
Publications and source records attributed to M I Pitman.
Various arthroscopic meniscal repair techniques have been developed in recent years to preserve meniscal function. We report the case of a patient with a failed arthroscopic meniscal repair demonstrating an inflammatory foreign-body reaction to bioabsorbable meniscal arrows.
We have developed a novel, two-layered, collagen matrix seeded with chondrocytes for repair of articular cartilage. It consists of a dense collagen layer which is in contact with bone and a porous matrix to support the seeded chondrocytes. The matrices were implanted in rabbit femoral trochleas for up to 24 weeks. The control groups received either a matrix without cells or no implant. The best histological repair was seen with cell-seeded implants. The permeability and glycosaminoglycan content of both implant groups were nearly normal, but were significantly less in tissue from empty defects. The type-II collagen content of the seeded implants was normal. For unseeded implants it was 74.3% of the normal and for empty defects only 20%. The current treatments for articular injury often result in a fibrous repair which deteriorates with time. This bilayer implant allowed sustained hyaline-like repair of articular defects during the entire six-month period of observation.
The purpose of this study was to observe the difference in healing of full-thickness articular cartilage defects treated with burr arthroplasty versus subchondral drilling. Cartilage was shaved off the medial femoral condyles of 39 rabbits without penetrating the subchondral plate. In left knees, two 2.0-mm holes were drilled into the condyle until bleeding was obtained. Right knees underwent a burr arthroplasty until punctate bleeding was observed. Animals were sacrificed at 6, 12, and 24 weeks postoperatively. Joint resurfacing and degenerative changes were evaluated grossly and histologically. Degenerative changes in the cartilage surface were observed with both treatments. Rabbits undergoing subchondral drilling had increased fibrocartilaginous healing with time, with a slight increase in degenerative changes. With burr arthroplasty, there was significant decrease in cartilaginous coverage of the exposed surface as well as progressive increase in degenerative changes. Although both techniques were suboptimal, histological evidence at 6 months suggests that subchondral drilling may result in a longer-lived repair than abrasion arthroplasty in the treatment of full-thickness lesions.
Most of the investigation of the properties of the anterior cruciate ligament (ACL) has focused on its biomechanical functions. There has been increasing interest in the study of the possible neuroreceptor function of the ACL and its role in providing important proprioceptive feedback. Anatomic and histologic studies in humans have shown the presence of neuroreceptors within the anterior cruciate ligament. Indirect evidence exists that proprioception is diminished in the ACL-deficient knee. However, direct evidence in humans of the actual origin of the afferent impulses from within the ACL itself is lacking. Measurement of direct electrical afferent activity, occurring on stimulation of the ACL, should provide this evidence. Somatosensory evoked potentials (SEP) measure the electric potentials evoked in the cerebral cortex upon stimulation of a peripheral neuroreceptor. Carried by the posterior columns of the spinal cord, they reflect activity of the proprioceptive fibers. During arthroscopic procedures performed on nine patients, the normal ACL was stimulated by the use of electrodes applied to the femoral end, midsubstance, and tibial end, and cortical potentials thus evoked were recorded. In all cases, SEPs were recorded at the cerebral cortex upon stimulation of the ACL. The greatest potentials were reported upon stimulation of the midsubstance of the ligament. These findings provide direct evidence for, and strongly support the presence of, active proprioceptive receptors within the intact anterior cruciate ligament of the human knee.
Reports of laser energy applied to soft tissues in vitro and in vivo suggest both stimulation and inhibition of specific metabolic processes, depending on the type of laser, the energy density (ED) used, the mode of delivery, and type of tissue studied. An earlier in vitro study of Nd:YAG laser irradiation of articular cartilage indicated stimulation of both matrix and DNA synthesis for 6 days following laser exposure. In vivo reports on the ability of Nd:YAG laser energy to stimulate the healing of partial-thickness cartilage defects are conflicting. In the present study, a noncontact continuous-wave Nd:YAG laser beam of varying EDs was applied to full-thickness adult articular cartilage explants maintained in organ culture; the metabolic processes of chondrocyte DNA synthesis and matrix synthesis were followed over 2 weeks. For both canine and bovine cartilage, low-levels of laser energy (ED 51-127 J/cm2) stimulated matrix synthesis at 6-7 days following laser exposure, with a concomitant decrease in baseline DNA synthesis. By 12-14 days, however, these dose-dependent effects were no longer seen, with no significant differences from control noted for any of the laser energies studied. Histologic analysis of the cartilage explants following laser exposure showed no significant differences in cell number or morphology between sample and control groups; however, a decrease in matrix proteoglycan staining was seen in the highest laser energy group at all time points. These findings indicate that exposure to low-level noncontact Nd:YAG laser energy promotes a significant stimulation of cartilage matrix synthesis. However, a single exposure may not be sufficient to promote a sustained upregulation of cartilage metabolism.(ABSTRACT TRUNCATED AT 250 WORDS)
Using the knee joints of New Zealand White rabbits, a baseline study was made to determine the intrinsic capability of cartilage for healing defects that do not fracture the subchondral plate. A second experiment examined the effect of autologous chondrocytes grown in vitro on the healing rate of these defects. To determine whether any of the reconstituted cartilage resulted from the chondrocyte graft, a third experiment was conducted involving grafts with chondrocytes that had been labeled prior to grafting with a nuclear tracer. Results were evaluated using both qualitative and quantitative light microscopy. Macroscopic results from grafted specimens displayed a marked decrease in synovitis and other degenerative changes. In defects that had received transplants, a significant amount of cartilage was reconstituted (82%) compared to ungrafted controls (18%). Autoradiography on reconstituted cartilage showed that there were labeled cells incorporated into the repair matrix.
Two commercial adhesive preparations--fibrin glue and mussel adhesive protein (MAP)--were tested in-vivo for their ability to fix a chondrocyte allograft internally. While results for the fibrin, including additional testing for chondro inductive/conductive properties, were at best inconclusive, the results for MAP are highly promising.
With the increase in the use of shoulder arthroscopy in the past decade, there has been an increased awareness of complications. Reports of the occurrence of transient neuropraxia indicate an incidence of 10%-30%. The recording of somatosensory evoked potentials (SEP) for the study and functional monitoring of the sensory pathway is well accepted as a reproducible method of monitoring peripheral nerve and spinal cord function during surgery. SEPs were recorded during shoulder arthroscopy in 20 patients to monitor the musculocutaneous nerve, ulnar nerve, and either the median or radial nerve. In all 20 cases, abnormal SEPs of the musculocutaneous nerve were demonstrated. In 16 cases, this was produced upon initial joint distention, and in 15 cases, by traction; in 11, by longitudinal traction of greater than or equal to 12 lb, and in six by perpendicular traction of greater than or equal to 7 lb. In 10 patients, there were varying combinations of median, ulnar, and radial nerve involvement. There were two cases of clinical neuropraxia in this series. One resolved in 24 h and one in 48 h. The conclusion is that there is a real potential for neurologic damage during shoulder arthroscopy and that the musculocutaneous nerve is the most vulnerable. Factors responsible include joint distention, excessive traction, and extravasation of fluid. The use of SEPs provides a reliable means for monitoring the neurologic status of the extremity during shoulder arthroscopy.
This report describes the in-vitro use of mussel adhesive protein (MAP) as a possible adjunct to chondrocyte allograft surgery. Experiments were performed to define the attachment kinetics of chondrocyte onto bone using MAP, and to assess the biocompatibility of this material in vitro. The results indicate that MAP is biocompatible and may have future clinical applications in orthopaedic procedures.
It is important to remember that knee disorders seen in the elderly are distinctly different from those seen in the younger individual. In the elderly, the problems are generally the result of chronic processes and, occasionally, an acute process on top of a chronic disorder. A careful history and physical examination should enable the physician in most cases to make the correct diagnosis. However, at times, specific laboratory studies may be ordered to confirm the diagnosis. In part I of this two-part review of geriatric knee disorders, the authors will focus on the anatomy, history, and physical examination of the knee joint. Common knee disorders specific to the elderly will be discussed in part II.
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Ligament insufficiency due to athletic injury is widely recognized and reported as an etiological factor in knee joint instability. It was recognized that a patient presenting with knee joint instability due to past hockey injury to the anterior cruciate ligament, subsequently verified surgically, was able to voluntarily and actively perform the pivot shift maneuver with his knee as a result of his anterolateral rotatory instability. During a subsequent 6 month period, three other patients with similar anterior cruciate deficiencies presented with this same active pivot shift maneuver phenomenon. A study was designed to determine the active muscle or muscles involved in this abnormal active motion in the knee joint with anterolateral rotatory instability. Electromyographic studies with needle electrodes were conducted on a variety of muscles in four subjects presenting with the instability. Results indicated that the popliteus muscle plays a major role in the active performance of the pivot shift maneuver.
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Isolated posterolateral rotatory instability of the knee is an uncommon injury pattern that may result in significant degrees of functional disability. This injury complex can be a challenging diagnostic and therapeutic problem for the orthopaedic surgeon. The presence of associated ligamentous and soft-tissue injuries, resulting in combined instability patterns, further complicates management. The results of recent research have enhanced our understanding of the complex anatomy and biomechanics of the posterolateral aspect of the knee. Numerous surgical techniques have been described for both repair and reconstruction of the injured posterolateral structures; however, long-term functional results have been only moderately successful.