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Biomedical subjects

M Ia Spivak

Publications and source records attributed to M Ia Spivak.

At least 19 recordsLinked to original sources
PubMed1999

[The determination of the cellular immunity indices in papillomavirus infection of the female genitalia].

The authors studied the impact of HPV infection and HPV-associated cellular intraepithelial neoplasia (CIN) and cancer in situ on indicators of cellular immunity, such as levels of T(CD3+)- and B (CD19+)-lymphocytes as well as those of their subpopulations (CD4+, CD8+, CD3+DR+, CD3-DR+). Female patients presenting with benign states and CIN II as well as those with CIN III and cancer in situ induced by HPV exhibit an apparent decrease in CD3+DR+T-lymphocyte count in their peripheral blood. In benign states and CIN I as well as in CIN III an cancer in situ levels of CD4(+)-cells are noted to be on the decrease while those of CD8+ T-lymphocytes get appreciably increased. Female patients with benign states and CIN I as well as those with CIN III and cancer in situ have a reduction in the index CD4/CD8 compared to the values in controls. The results obtained may have a prognostic significance and will, we believe, help in developing methodological approaches to the immunocorrection of HPV infections and HPV-associated disorders of female genital organs.

Adolescent↗
PubMedDate not supplied

[Current methods of laboratory diagnosis of HIV infection].

The paper is a review of scientific data obtained in 1990-1998 and concerning the up-to-date diagnostical approaches used in the laboratory practice. The main widely used methods of HIV-specific antigens and antibodies detection in biological fluids are discussed. The paper deals with the basic methods of HIV-infection diagnosis, an analysis of their specificity, sensitivity, their advantages and possible pitfalls. The principal approach permitting one to improve the current test-systems is the elaboration of automated and express tests side by side with the increasing of their sensitivity and specificity.

AIDS Serodiagnosis↗
PubMedDate not supplied

[Clinical study of anti-leukotriene therapy with montelukast in bronchial asthma patients].

Clinical and functional efficacy of the antileukotriene agent montelucast (singulair) was investigated in 30 patients with moderately severe bronchial asthma. Patients with a positive FEV1 response to montelukast (61.1%) demonstrated a higher blood level of IgE and sputum eosinophilia. Enhancement of bronchodilatatory effect secondary to the use of beta 2-agonist has been recordable in the wake of a 24-day montelucast treatment (10 mg o.d.).

Acetates↗
PubMedDate not supplied

[Prognosis of pretreatment outcome in patients with acute glomerulonephritis after an immunological review].

Expediency has been shown for the immunological investigation to be done in those patients with acute glomerulonephritis in the nephrosis stage before the start of treatment to find out relevant approaches to such patients. The most informative parameters characterizing immunity include the peripheral blood absolute content T-lymphocytes, relative and absolute content of B-lymphocytes in the peripheral blood, IgA, IgG in blood plasma. However, to prognosticate near term clinical efficiency of treatment with immunodepressive drugs it is essential that all basic indices for the cell-bound and humoral immunity be taken account of.

Acute Disease↗
PubMedDate not supplied

[Effectiveness of short courses of fasting in pre-asthma and asthma patients].

In patients with preasthma and bronchial asthma, short-term courses of fasting dietotherapy (FDT) with a 7-day fasting period proved to be effective, as evidenced by clinical-and-functional and laboratory investigations. The incidence rate of viral infections was much lower with short-term courses compared to long-term courses. Too low an effect, if any, with FDT short-term courses can be explained by excess of the patient's body weight. Short-term FDT courses with a 3-day fasting period have been found out to result in a significant decrease in the level of anxiety, as measured by Spilberger Anxiety Inventory.

Adult↗
PubMedDate not supplied

[Interferon inducers--from theory to practice].

The authors present up-to-date information concerning different inducers of different interferon types in vivo and in vitro. Interactions between the structure and interferon-inducing activity of high and low molecular weight inducers are in the focus of this paper. There are also a lot of data, concerning different biological properties of interferon-inducing compounds. Different aspects of inducers use as antiviral and immunomodulating drugs are also discussed.

Animals↗
PubMedDate not supplied

[Obtaining of new monoclonal antibodies against human immunoglobulins of class G, suitable for use in diagnostics of infectious diseases].

An original set of 17 clones of hybridomas-producers of monoclonal antibodies (MCAB) against Fc-fragment of human IgG has been obtained. The possibility of using horseradish peroxidase conjugates of the obtained MCAB as a part of ELISA tests systems of the company "DiaProphMed" for diagnostics of syphilis, rubella, toxoplasmosis and cytomegalovirus infections has been comparatively studied. It has been shown, that the application of peroxidase conjugates of two monoclonal antibodies (156C10 and 153H11) as the part of five diagnostic ELISA test systems provides high specificity and sensitivity.

Animals↗
PubMedDate not supplied

[Optimization of cultivation conditions of recombinant Escherichia coli strain KPR-42--producer of human gamma-interferon].

Cultivation conditions for recombinant Escherichia coli strain KPR-42 producing human gamma-interferon in liquid nutrient medium were optimized. Glucose content in the medium was optimized by the reason that glucose catabolism products cause the medium acidification. That permitted to prolong fermentation time and increase recombinant protein content in the cells by 23-25%, avoiding expendable enrichment of the medium by nutrient components.

Biotechnology↗