PubMed Health⌕ Search

Biomedical subjects

M Ianconescu

Publications and source records attributed to M Ianconescu.

26 records · Page 2Linked to original sources
PubMedDate not supplied

Hemorrhagic enteritis of turkeys in California: serologic study of hemorrhagic enteritis virus antibody with an enzyme-linked immunosorbent assay.

The incidence of hemorrhagic enteritis (HE) infection in California turkeys was studied by testing 2220 turkey blood samples from 173 flocks for HE virus (HEV) antibody by the enzyme-linked immunosorbent assay (ELISA). Maternal antibody was detected at 1 day of age in all flocks tested, and it vanished after 3 weeks. Acquired HEV antibody appeared at 8 to 10 weeks, and 100% of the meat and breeder turkey flocks were positive after 11 weeks of age. HEV infection occurred earlier in the meat flocks than in the breeder flocks, and it also occurred earlier during summer than during the fall and winter months.

Animals↗
PubMedDate not supplied

Paramyxovirus type 3 (PMV-3) in California turkeys: serologic study of PMV-3 antibody with an enzyme-linked immunosorbent assay.

An enzyme-linked immunosorbent assay (ELISA) was developed for detecting paramyxovirus type 3 (PMV-3) antibody. This test had a higher sensitivity than the hemagglutination-inhibition test, and no cross-reactivity with various paramyxoviruses or avian pathogens was detected when the sera were tested diluted 1:200. The incidence of PMV-3 infection in California was studied by testing 2037 turkey sera from 174 meat and breeder flocks for the presence of PMV-3 antibody using the ELISA. The age at which the infection occurs was around 5 to 8 weeks for meat flocks and 10 to 12 weeks for breeder flocks. Infection with the PMV-3, as determined serologically, was more frequent than manifested cases of disease, and 95.2% of the flocks aged over 11 weeks had PMV-3 antibody. No typical manifestations of PMV-3 disease (respiratory disease plus drop in egg production) were observed, probably because of the early infection which occurred before laying age.

Animals↗
PubMedDate not supplied

An enzyme-linked immunosorbent assay for detection of hemorrhagic enteritis virus and associated antibodies.

An enzyme-linked immunosorbent assay (ELISA) that detects antibodies specific for type II avian adenoviruses is described. Freon-treated extracts from spleens of hemorrhagic enteritis (HEV)-infected poults were a better source of antigen than the cell-culture-propagated virus. Antigen-coated microtiter wells treated with bovine serum albumin reduced nonspecific adsorption of immunoglobulins. The ELISA readily detected antibodies in hemorrhagic-enteritis- and marble-spleen-disease-infected field flocks of turkeys and pheasants. Antibody was also detected 1 week after vaccination of 2-week-old poults with cell-culture-prepared hemorrhagic enteritis vaccine. The assay was specific and more sensitive than agar-gel-precipitation and virus-neutralization tests. HEV-related antigens in infected spleens were also detected indirectly in a blocking ELISA by removal of HEV antibodies (Ab) from stock (Ab+) serum through antigen-antibody complex formation. Extracts from HEV-infected poults reduced specific antibody reactivities in HEV-antigen-coated wells, whereas extracts from HEV-challenged poults previously vaccinated with attenuated virus did not remove HEV antibodies.

Animals↗
PubMedDate not supplied

Effect of lymphoproliferative disease virus and of niridazole on the in vitro blastogenic response of peripheral blood lymphocytes of turkeys.

Turkeys inoculated at 5 weeks of age with lymphoproliferative disease (LPD) virus developed typical lesions in the spleen, thymus, and pancreas. The in vitro blastogenic response of peripheral blood lymphocytes to the mitogens phytohemagglutinin and concanavalin A was drastically (up to 90%) suppressed in the inoculated turkeys 1 to 4 weeks postinoculation compared with uninoculated controls, and even at 11 weeks the response was about 50% inhibited. A lethal (about LD33) dose of antihelminthic drug niridazole, 100 mg/kg given each day for 3 days to 4-week-old turkeys, caused a transient inhibition of the blastogenic response within 32 days of treatment, which was less pronounced than that observed in turkeys inoculated with LPD virus, whether pretreated with niridazole or not. Virus-associated reverse transcriptase activity in the plasma was significantly higher in the turkeys pretreated with niridazole, and LPD lesions developed to the same extent in the untreated and treated groups, as determined 9 weeks post virus inoculation. A sublethal dose of niridazole, 50 mg/kg given each day for 4 days, did not suppress the blastogenic response to mitogens at any time determined (starting 10 days post-treatment) and did not affect the pathogenesis of LPD and the viremia. Body weights were significantly decreased by virus infection and by treatment with lethal doses of niridazole.

Animals↗
PubMedDate not supplied

In vitro and in vivo investigations on reticuloendotheliosis virus infection.

Experimental transmission of reticuloendotheliosis (RE) in 1-day-old SPF chickens was achieved by intraperitoneal inoculation of cell-associated and cell-free RE virus (REV). REV-inoculated chickens exhibited an immune response throughout the entire observation period. Viremia persisted for 5--6 months in chickens hatched from REV-inoculated SPF embryonated eggs. The natural occurrence of RE was detected in turkey flocks of farms Bc.

Animals↗
PubMedDate not supplied

Turkey meningo-encephalitis: a general review.

Turkey meningo-encephalitis (TME), a neuro-paralytic disease of the adult turkey caused by an Arbovirus, was described in 1960 in Israel, and not yet reported elsewhere. The seasonal outbreaks caused a high morbidity and a mortality up to 80%. A tendency toward spread has been found in the last two years. The TME virus was attenuated sucessfully by adapting it to a new host, the Japanese quail, and a vaccine prepared with this attenuated virus is effective in immunizing turkeys to the disease.

Animals↗