Biomedical subjects

M Illert

Publications and source records attributed to M Illert.

At least 37 records · Page 2Linked to original sources

PubMed1996

On the mechanism of the post-activation depression of the H-reflex in human subjects.

It was demonstrated that the soleus H-reflex was depressed for more than 10 s following a preceding passive dorsiflexion of the ankle joint. This depression was caused by activation of large-diameter afferents with receptors located in the leg muscles, as an ischaemic block of large-diameter fibres just below the knee joint abolished the depression, whereas a similar block just proximal to the ankle joint was ineffective. The depression of the H-reflex was not caused by changes in motoneuronal excitability, as motor-evoked potentials by magnetic brain stimulation were not depressed by the same passive dorsiflexion. Therefore it was concluded that the long-lasting depression is due to mechanisms acting at presynaptic level. The transmission of the monosynaptic Ia excitation from the femoral nerve to soleus motoneurones was not depressed by the ankle dorsiflexion. The depression thus seems to be confined to those afferents that were activated by the conditioning dorsiflexion. In parallel experiments on decerebrate cats, more invasive methods have complemented the indirect techniques used in the experiments on human subjects. A similar long-lasting depression of triceps surae monosynaptic reflexes was evoked by a preceding conditioning stimulation of the triceps surae Ia afferents. This depression was accompanied by a reduction of the monosynaptic Ia excitatory postsynaptic potential recorded intracellularly in triceps surae motoneurones, but not by changes in the input resistance or membrane potential in the motoneurones. Stimulation of separate branches within the triceps surae nerve demonstrated that the depression is confined to those afferents that were activated by the conditioning stimulus. This long-lasting depression was not accompanied by a dorsal root potential. It is concluded that the long-lasting depression is probably caused by a presynaptic effect, but different from the "classical" GABAergic presynaptic inhibition which is widely distributed among afferent fibres and accompanied by dorsal root potentials. It is more probably related to the phenomenon of a reduced transmitter release from previously activated fibres, i.e. a homosynaptic post-activation depression. The consequences of this post-activation depression for the interpretation of results on spinal mechanisms during voluntary movements in man are discussed.

Adult↗

PubMed1996

Kinematic analysis of the cat shoulder girdle during treadmill locomotion: an X-ray study.

A quantitative kinematic analysis of the movements of the shoulder girdle in the three dimensions of space during treadmill locomotion (velocity range 0.33-1.2 m/s) was performed in two cats. Since the movement patterns of the scapula and the humeroscapular joint can only vaguely be estimated through the overlying skin we used implanted metal spheres placed on the scapula in combination with three-dimensional pulsed X-ray cinematography (time resolution 20 ms) to reconstruct the excursions of the scapula, the humerus and the elbow and to calculate the respective angular amplitudes and velocities. The movements of the scapula relative to the Th4 spinous process consist of four major components:(i) a monophasic flexion (caudocranial movement of glenoid fossa during swing)/extension (craniocaudal movement of the glenoid fossa during stance) sequence, the fulcrum for which sequence is situated near the vertebral border of the scapula at the medial elongation of the scapular spine; (ii) a vertical monophasic up/down sequence of the fulcrum relative to the trunk, the highest vertical position being reached during mid-stance and the lowest vertical position during mid-swing; (iii) a biphasic abduction/adduction sequence during swing and during stance respectively; and (iv) small rotations along the scapular spine. The trajectory recordings of the scapula indicate that the scapula yields relative to the trunk under the body weight after ground contact. The angular excursions of the humeroscapular joint consist of : (i) a flexion/extension sequence during swing, a yield after ground contact and a final extension at the end of stance; (ii) an adduction and outward rotation during the early swing phase flexion; (iii) an abduction and inward rotation during the late swing phase extension; and (iv) an adduction during the yield with only minor rotations during the whole stance phase. The movement patterns are discussed in view of the muscular synergies necessary to guide the scapula and the humerus during stance and swing, and in relation to the implications for the organization of these patterns in spinal neuronal systems.

Animals↗

PubMed1996

Monosynaptic Ia pathways and motor behaviour of the cat distal forelimb.

The projection pattern from large muscle spindle afferents (Ia) onto cat alpha-motoneurones innervating the elbow, wrist and digit muscles is correlated with new electromyographical and x-ray kinematic data on the motor behaviour of the forelimb. This correlation suggests that the Ia-synergistic groups may be one substrate to organize movement components mobilized in motor behaviour. This hypothesis is based on the data on the active stabilization of the radio-ulnar joints during the stance phase, on the data on the Ia-organization of a motor nucleus taking part in different motor behaviours and on the results showing how the Ia convergence onto the long digit extensor muscles fits the execution of finely tuned movements at ball joints.

Animals↗

PubMed1996

Beta innervation and recurrent inhibition: a hypothesis for manipulatory and postural control.

The innervation of muscle spindles by skeletofusimotor (beta) axons in functionally different muscles of the cat forelimb has been correlated with the neural circuitry of the respective motor nuclei, morphological characteristics of their motoneurones and the innervated muscle fibres. In long digt extensor muscles a high degree of beta-innervation (more than 70%) and of fast contracting muscle fibres (more than 70% IIB fibres) correlates with specific projections of their Ia muscle spindle afferents, with the absence of a recurrent inhibitory system, with cell body diameters in the range of small alpha-motoneurones and with a short duration of their after-hyperpolarisations. In contrast, the investigated elbow muscles display a low degree of beta-innervation (41-47%) irrespective of their fibre type composition, their Ia afferent fibres show a divergent projection pattern and their motor nuclei a distinct recurrent inhibitory system. We suggest that for the distal muscles the specific combination of these different characteristics serves the control of manipulative movements, whereas for the proximal muscles the contrasting characteristics serve the control of posture and locomotion. This hypothesis is discussed in view of the phylogenetic development of motor control.

Animals↗

PubMed1995

Skeletofusimotor (beta) innervation of proximal and distal forelimb muscles of the cat.

The innervation of muscle spindles by skeletofusimotor (beta) axons has been compared in two long digit extensor muscles with three elbow extensor muscles of the cat forelimb. The proportion of muscle spindle poles with p1-endplates was analysed in silver impregnated teased material. The proximal and distal muscles displayed a significant difference in their proportion of muscle spindle poles with p1-endplates. The distal muscles had an estimated proportion of more than 70% beta innervation of their muscle spindles, the proximal muscles of 41-47%. This difference is discussed with respect to the different circuitry of the motoneurones. We suggest that the high proportion of beta-innervated spindles in distal muscles obtained with the absence of a recurrent inhibitory system in the innervating motoneurones [18] serves the execution of manipulative movements.

Animals↗

PubMed1994

X-ray study of the cat hindlimb during treadmill locomotion.

With pulsed X-ray cinematography we have analysed the angular excursions of the distal hindlimb joints (proximal interphalangeal, PIP; metatarsophalangeal, MTP; ankle) in cats walking on a treadmill. These distal joints transmit the body weight and the dynamic forces onto the ground. We have included the knee and hip joints in the analysis to relate the angular excursions of the proximal and distal joints and to verify the data previously obtained with external markers on the kinematics of the proximal joints. At the beginning of the stance phase the PIP joints flexed rapidly, the MTP joints extended slowly and the ankle and knee yielded under body weight. Whereas the PIP joints maintained a rather constant angular position of approximately 75 degrees throughout the stance phase, extension continued in the MTP joints from approximately 230 degrees at touch-down to approximately 270 degrees at the end of the stance phase. Around 50 ms before lift-off the MTP joints flexed rapidly. Early (approximately 30 ms) after lift-off this flexion changed into a slow extension. The PIP joints extended swiftly at the stance-swing transition and moderately at the end of the swing phase. During the middle part of the swing phase they flexed slowly. Small rotatory movements around the long axis of the foot took place in the last 100 ms of the swing phase. The results of this study on the distal joints are discussed in relation to the placing of the paw, to the translation of forward propulsion into a MTP movement and to the lifting of the paw (conventionally described as toe curling). They show a differentiated mechanical interaction between the different distal limb joints during these different phases, which must be known in detail to interpret the corresponding electromyographic data and to understand how the hip is moved forward over the MTP joints which serve as the final pivot during stance.

Animals↗

PubMed1994

X-ray kinematography as a tool for investigations of distal limb movements of the cat.

X-ray kinematography was used to investigate the kinematics of cat distal forelimb joints during motor behaviour. These joints are not accessible for instrumentation with external markers normally used in conventional motion analysis systems. To trace the movements in space two X-ray systems positioned rectangularly to each other illuminated the forelimb quasi-simultaneously with pulsed X-ray shots (time resolution: 20 ms). A mathematical model was developed for 3-dimensional reconstruction of the object and includes procedures for correction of image distortions, e.g., pincushion distortions and rotation of the image due to interaction of the earth's magnetic field with the electron optics of the image amplifiers. Accuracy of image correction and object reconstruction is +/- 1 pixel, corresponding to +/- 0.5 mm in space which is sufficient for investigation of the kinematics of cat distal forelimb joints. The approach described is of general relevance and useful in kinematic investigations where the structures under study are not directly accessible to external instrumentation with markers.

Animals↗

PubMed1992

Reciprocal inhibition of forearm flexor muscles in spasmodic torticollis.

Reciprocal inhibition between forearm extensor and flexor muscles was tested by means of an H-reflex technique in patients with spasmodic torticollis and normal controls. In both, patients and controls three different phases of reciprocal inhibition could be demonstrated with maximal inhibition at conditioning test intervals of 0 ms, 15 ms and 100 ms, respectively. However, the quantitative amount of this inhibition was different for the patients and the controls. Significantly less inhibition was found for the second and the third phase of reciprocal inhibition in the patient group. Discriminant analysis showed a clear separation between normal subjects and patients if the amount of reciprocal inhibition of the second and third phase were taken into account. We were not able to detect any side differences neither for the patients nor for the controls. The findings demonstrate a functional disturbance of motor control mechanisms of a clinically unaffected extremity in spasmodic torticollis. This is believed to reflect a bilateral disturbance most likely within the basal ganglia or their outflow. Therefore, our data support the idea, that spasmodic torticollis is associated with or even due to a generalized rather than a focal disturbance of motor control mechanisms.

Analysis of Variance↗

PubMed1992

Electroencephalography and evoked potentials: a PC-based analysis program for laboratory courses in physiology.

A computer program (EEG Analysis) was developed for the preclinical laboratory course in physiology held for medical and dental students. It offers an off-line analysis of a set of typical and frequently occurring physiological and pathological electroencephalogram (EEG) and evoked potential (EP) recordings, which are stored in an IBM-compatible personal computer (PC) system. The users are requested to measure and analyze the data sets and to work through a base of questions relevant in the frame of the particular topic. The program is structured in several exercises: calibration, pickup of non-EEG signals (eye movements, chewing), waveforms in EEG recordings from awake subjects (alpha-waves, beta-waves), desynchronization of cerebral activity (visual activation, acoustic activation, mental activation), habituation of cerebral activity upon acoustic stimuli, EEG recordings from asleep subjects (different sleep stages, sleep-specific EEG signals), epileptic seizures, and EPs (principle of averaging, visually evoked potentials in different cortical areas). The program runs under MS-DOS and is network capable. The software structure ensures maximal flexibility for rapid changes and adaptations of the program to specific needs of a particular EEG course. The program has been used for three years, and the response from > 800 students has been consistently positive.

Acoustic Stimulation↗

PubMed1991

Absence of recurrent axon collaterals in motoneurones to the extrinsic digit extensor muscles of the cat forelimb.

Forelimb alpha-motoneurones were intracellularly recorded in anaesthetized cats and iontophoretically filled with horseradish peroxidase (HRP). All motoneurones to the elbow flexors, elbow extensor and to the extensor carpi radialis muscles displayed in parallel homonymous recurrent inhibitory postsynaptic potentials (RIPSPs) and axon collaterals. Homonymous RIPSPs and axon collaterals were missing in the nuclei to the long digit extensor muscles. Two populations of motoneurones, with and without recurrent axon collaterals, seem to be present in the extensor carpi ulnaris motor nucleus. These results are consistent with the hypothesis that the motoneurones to the extrinsic digit extensors lack a recurrent axonal system. This indicates that the contribution of the recurrent Renshaw systems to motor control may be more complex than hitherto assumed.

Animals↗

PubMed1989

Distribution of recurrent inhibition in the cat forelimb.

This chapter reviews experiments on the distribution of recurrent pathways from motor axon collaterals to alpha motoneurones in the brachial enlargement of the cat. In anaesthetized cats intracellular recording from identified forelimb motoneurones was used to reveal the pattern of recurrent inhibition or excitation following stimulation of muscle nerves. The recurrent connections of the motor nuclei acting on the elbow follow the tight mechanical agonism of the muscles involved. Extensive bidirectional recurrent inhibitory connections were found between motor nuclei innervating elbow and wrist muscles. It is suggested that one group of these connections supports the organization of limb extension, the other group organization of limb flexion. The supinator and the pronator teres motornuclei have identical recurrent connections. Co-convergence from elbow flexor and extensor motornuclei in one and the same motoneurone is frequent. It is suggested that this pattern may serve the stabilization of the radio-ulnar plane. Neither homonymous nor heteronymous recurrent actions were observed in the radial motornuclei acting on the digits, which suggests a lack of recurrent axon collaterals in these nuclei. These results draw attention to the fact that recurrent inhibition is not evenly distributed between all limb motor nuclei.

Animals↗

PubMed1989

Pattern of monosynaptic Ia connections in the cat forelimb.

1. In anaesthetized cats intracellular records were obtained from antidromically identified motoneurones. The motor nuclei to the elbow extensor and flexor muscles and to the muscles innervated by the deep radial, ulnar and median nerves were investigated. The maximum Ia EPSPs from electrical stimulation of various peripheral nerves were measured. The characteristic convergence and projection patterns to each motor nucleus were established from pooled data. 2. The total aggregates of the Ia EPSPs between the different motor nuclei ranged from 3.5 to 11.7 mV. The smallest aggregates were found in the nuclei to the digit muscles. The ratio of the heteronymous versus homonymous EPSP amplitudes varied between 3.9 and 0.5. A general rule which would govern the distribution of the EPSP aggregates, such as a proximo-distal gradient, was not observed. 3. The Ia connections followed a complex but highly organized pattern. Bidirectional and unidirectional pathways were present. In many cases the convergence pattern of a motor nucleus included muscles acting at different joints. The connections of one nucleus were not necessarily restricted to one side of the limb, but could cross the radio-ulnar plane. 4. Muscles with similar actions onto the same joint were interconnected with bidirectional, rather balanced Ia pathways. Such relations were also present between close functional synergists and then often extended across several joints. The relations between the anatomical extensors of wrist and digits were graded according to the neighbourhood of these muscles. It is suggested that this reflects the graded mechanical synergism in the wrist action of these muscles. 5. A large number of unidirectional or strongly skewed bidirectional Ia pathways project from proximal to distal muscles. It is suggested that they may serve a readjustment of distal joints during changes in the position of proximal ones (e.g. stabilization of the position of the radio-ulnar plane during elbow extension in case of the undirectional projections onto supinator and abductor pollicis longus motoneurones). 6. The motor nuclei to some multifunctional muscles display a negative correlation between different heteronymous Ia inputs: motoneurones with a large input from one muscle show a significant tendency to receive a smaller input from another muscle and vice versa. This organization leads to subpopulations of neurones with different convergence patterns within the same motor nucleus. 7. Motor nuclei with bidirectional Ia relations between each other displayed similar convergence and projection patterns. They were combined into 'Ia synergistic groups.' One motor nucleus may belong to several groups.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

PubMed1989