Biomedical subjects
Publications and source records attributed to M Iosefsohn.
We compared results of an enzymic assay for alpha-amylase (EC 3.2 1.1), adapted to a centrifugal analyzer (GEMSAEC), with results by the manual dyed-starch (Phadebas) amylase assay. We assessed linearity and precision, and the stability of the reagent. The kinetic procedure is rapid, precise, requires only 25 microliter of serum, and thus is evidently suited for both pediatric and emergency use.
We compared the Beckman Cl/CO2 Analyzer to the American Instrument Company chloride titrator for measurement of chloride in serum, sweat, and spinal fluid. We also compared it to the Natelson gasometer and a modified continuous-flow (AutoAnalyzer) technique for measurement of carbon dioxide in serum. Effects of bilirubin and hemoglobin on results for both the CO2, and Cl were negligible. The linearity, stability, and precision of the equipment was studied. We believe the Beckman Cl/CO2 Analyzer to be ideal for simultaneous measurement of chloride and carbon dioxide in a pediatric or emergency-determination setting.