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Biomedical subjects

M Ismail

Publications and source records attributed to M Ismail.

At least 145 records · Page 8Linked to original sources
PubMed1979

A comparison between a new serological method, thin layer immunoassay (TIA), and the enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in schistosomiasis.

A comparison has been made of a new serological method, thin layer immunoassay (TIA), and an established method, enzyme-linked immunosorbent assay (ELISA), in the detection and quantification of antibodies in schistosomiasis. Using sera from known S. haematobium and S. mansoni cases, the performance of the two tests was almost identical; 95.6% positive for TIA and 96.4% by ELISA. TIA however produced a small number of false positives with sera from other helminth infections whereas ELISA gave none. There was excellent correlation between the tests in the quantification of anti-S. haematobium antibodies, both in human cases and in infected baboons. TIA has the advantage of being extremely simple to perform, but has the disadvantage of requiring a higher concentration of antigens.

Animals↗
PubMed1975

Hypothermic effect of cocaine in rats.

Cocaine injected intraperitoneally into rats resulted in a dose-dependent hypothermia. Intracerebral injection of smaller doses also produced a fall in body temperature. In rabbits and guinea-pigs, cocaine produced hyperthermia, in mice and chicks it produced hypothermia while inconsistent changes were produced in goats. Pre-treatment of rats with 6-hydroxydopamine, alpha-methyl-m-tyrosine or haloperidol significantly antagonized the cocaine hypothermia. Pre-treatment of the rats with either hyoscine or methscopolamine resulted in some but non-significant attenuation of the cocaineinduced hypothermia. Pre-treatment with p-chlorophenylalanine, however, did not modify the cocaine hypothermia. Pargyline pre-treatment significantly antagonized the hypothermic action of cocaine. It is suggested that cocaine may cause the release of noradrenaline centrally or it may potentiate its action by interfering with the uptake mechanism. It is also possible that cocaine may have a direct effect on the heat regulating centre in the hypothalamus.

Animals↗