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M Iu Obolenskaia

Publications and source records attributed to M Iu Obolenskaia.

9 recordsLinked to original sources
PubMed1991

[Cycle dependent expression of the gene coding for B2mRNA].

The relative contents of individual tissue specific RNA B2mRNAx was studied in the population of nuclear and poly(A)+ cytoplasmic RNA from the livers of intact, falsely operated and having suffered the partial liver resection rats. Dot-hybridization technique was used to study this transcript containing the transcribed copy of the B2 repetitive genetic element of rats. The expression of the gene coding for B2mRNAx takes place at the lower level in the liver induced to proliferation as compared with the one in the liver of intact animals. It is changed reproducibly in antiphase with the c-fos RNA and with major inclinations at the moments of cellular phases switch.

Animals↗
PubMedDate not supplied

[Pheno-and genotyping of a placental detoxication system in ecologically unfavorable regions of Ukraine].

During 1991-1993 period a study of detoxifying activity of the fetoplacental barrier and genotyping of the major detoxifying enzymes in it (CYP1A1 Ile462Val, GSTP1 Ile104Val, GSTM1 present/absent) was undertaken in different regions of Ukraine that were radioactively contaminated with summary effective equivalent annual expositional doses (SEEAED approximately 1.7 mSv (Group I) and 0.1-0.4 mSv (Group III), chemically polluted Zaporizzhia, monitored for ambient levels of benzo(a)pyrene (BP) (Group II) and Poltava that was judged as "clean" one (Group IV). Glutathione-S-transferase (GSTase) and glutathionereductase (GSSG-Rase) activities of cytosol and concentration of thiobarbituric acid reacting compounds (TBA-reactants) and reduced low-molecular weight thiols (rLMW thiols) were used as phenotype parameters. Cytosolic GSTase activities were nearly two times less in the samples from radioactively contaminated area (Group I, SEEAED approximately 1.7 mSv) and in chemically polluted area (Group II, mean BP level 12.3 ng/m3), compared with the groups III and IV. The highest level of TBA-reactants indicative of lipid peroxidation in response to radiation was observed in Group I, while the lowest level in Group IV. The level of rLMW thiols was 2.5-4 times more in Group II comparative with Groups I, III and IV. The frequency of the genotypes in all the investigated samples corresponds to that reported for Caucasians. For the combined exposure groups, individuals with the CYP1A1 (Ile462Val) genotype (n = 5) had significantly higher levels of GST, GSSG-R and TBA reacting compounds compared to individuals with the Ile462Ile genotype (n = 14 for TBA-reactants and n = 24--for GST and GSSG-R). Despite the challenge of small numbers of individuals, stratification by exposure group for Groups I, II and III indicated significantly higher GST levels in CYP1A1 (Ile462Val) variants from Groups II and III (n = 3) compared to the Ile462Ile variants (n = 17). The data demonstrate contributions by both exposure and genotype on the detoxification of radiation and chemical damage in the human placenta.

Base Sequence↗
PubMedDate not supplied

[Intracellular distribution of newly formed hepatocyte RNA in the 1st hours following partial hepatectomy].

Two periods in the activity of cell are distinguished during the first three hours after partial hepatectomy. The first period (about 0.5 h) is characterized by the activation of RNA synthesis in all the compartments of the cell, including mitochondria, and by approach of the latter to the nucleus, which is probably induced by the demand of a great quantity of energy for both the present and subsequent intranuclear synthesis. These data have permitted referring the response of hepatocytes in the first period to nonspecific ones. The second period (0.5-3 h) is distinguished by the differentiated response of hepatocytes to the proliferative stimulus.

Animals↗
PubMedDate not supplied

[Autoradiographic study of RNA synthesis and secretion into the cytoplasm during the early stages of liver regeneration].

Autoradiographic experiments with regeneration of rat liver showed that RNA transport from nucleus to cytoplasm occurs more quickly in 3h following partial hepatectomy while the total RNA synthesis increases insignificantly. Nucleolar RNA synthesis at this time grows in some hepatocytes and decreases in others though it does not differ on the average from the control one. By the 6th h after partial hepatectomy total RNA synthesis significantly increases simultaneously with the acceleration of RNA transport to cytoplasm. Nucleolar RNA synthesis intensifies in all hepatocytes.

Animals↗
PubMedDate not supplied

[Age characteristics of DNA replication in regenerating rat liver].

Incorporation of a label into double-stranded DNA as well as transformation of newly formed one-stranded DNA into the double-stranded DNA occurred slower in aged animals, as compared with adult ones, within the period of the most intensive DNA synthesis after partial hepatectomy. Dynamic characteristics of replication appear to alter with ageing.

Aging↗