Biomedical subjects
Publications and source records attributed to M J Aldred.
The literature recording cases of the rare juvenile hyaline fibromatosis is reviewed. Gingival enlargement is a regular feature of this entity. A case report and treatment of the gingival lesions are described, and the differential diagnosis and prognosis are discussed.
Adhesive tape applied to the fitting surface was used to study the early colonization of acrylic palates in six subjects. Streptococci were major constituents of the plaque accumulated on the tape over the first 3 days. Bacterial colonization of the tape discs was selective in that dextran-producing streptococci constituted a greater proportion of the total streptococcal count after 15 min compared with that of the initial sample and saliva, while the converse was true for Streptococcus salivarius. Scanning electron microscopy showed that denture plaque development started in grooves and depressions on the surface of the tape, and became more extensive with time. Comparison of the colonization of tape and acrylic discs in one individual demonstrated that the tape was a suitable model for in vivo colonization of dentures or orthodontic appliances.
Previous techniques used for the detection and quantitation of antibodies in body fluids may be inappropriate where only small volumes are available, or may not be sensitive enough to detect low levels of specific antibodies. An indirect ELISA technique has successfully been employed to estimate class-specific antibody levels to Streptococcus mutans in serum and secretions in a group of mothers and their neonates, and an attempt has been made to relate such levels to the presence or absence of active caries in the mothers. A high maternal serum IgG antibody level appears to exert a protective effect against dental caries. Antibody levels in maternal saliva and colostrum/breast milk showed no differences between the 2 groups. The presence of active caries in mothers was associated with an elevated IgA antibody level in neonatal saliva. Although ELISA permitted the detection of low levels of antibody in the small volumes of neonatal saliva collected, a further increase in sensitivity and specificity of the assay would be advantageous.
To our knowledge, this is the first report of calcium oxalate crystals in a cyst of the oral cavity. No underlying systemic disorder could be identified. It is proposed that this represents a dystrophic process, possibly related to inflammation, although the mechanism of the crystal formation is uncertain.
Breast milk antibodies have been shown to persist in the mouths of neonates for some hours following feeding. The decrease in concentration following a feed probably reflects the flushing action of salivary flow combined with swallowing.
A medium based upon tryptone, yeast extract, cystine (TYC) agar and incorporating bacitracin and sucrose has been evaluated for selective isolation of Streptococcus mutans. The effect of varying the concentrations of sucrose and bacitracin on the recovery of two standard strains was investigated. Growth of S. mutans NCTC 10449 was significantly inhibited by increasing concentrations of sucrose but was not affected by bacitracin; the reverse was seen with S. sobrinus strain 6715. The best compromise between recovery of the streptococci and growth of other organisms was obtained with a final sucrose concentration of 20% and bacitracin 0.2 units/ml. In comparison with three other selective media, this medium gave the highest recovery rate of standard strains, indicating that it is superior to mitis-salivarius bacitracin (MSB) agar for the recovery of S. mutans from saliva.
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A case is reported in which considerable external resorption of the crown of a lateral incisor occurred during storage in a buccal pouch.
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The prevalence of oral candidosis and the frequency of isolation of Candida albicans and its density and distribution have been determined in the mouths of 50 patients with diabetes mellitus and 50 healthy volunteers matched for age, sex, dental status and smoking habits. Three of the diabetic patients were found to have a chronic oral candidosis. According to an imprint culture technique, the oral carrier rate and density of C albicans were both higher in the diabetic group as a whole than in the control subjects. Smoking was associated with an increased prevalence of the yeast in diabetes mellitus. Diabetics wearing dentures had higher candidal density than those without a prosthesis. No differences in candidal status could be detected according to the degree of control of diabetes, mode of treatment, duration of diabetes or the patient's age. Local factors such as smoking and the presence of dentures, particularly when worn continuously, interact with diabetes mellitus in promoting candidal colonisation of the mouth. Attention to these predisposing factors could reduce the incidence of thrush in diabetics.
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This study of 20 hospice patients provided baseline information on oral and dental status in the terminally ill. For each patient, a questionnaire was completed, the mouth was examined, and an oral rinse and imprint cultures were collected to establish the prevalence and intra-oral density of yeasts, staphylococci and coliforms. Oral symptoms reported included disturbance of taste (26%), dysphagia (37%), soreness (42%), and dryness (58%). Of the 75% who wore dentures, 71% had difficulty with their prosthesis (es). A clinical diagnosis of oral candidiasis was made in 70% of subjects. The high prevalence of oral symptoms, denture problems, and candidiasis clearly affect the quality of remaining life in terminally ill patients.