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Biomedical subjects

M J Benson

Publications and source records attributed to M J Benson.

23 records · Page 2Linked to original sources
PubMed1980

Normal patterns of human upper small bowel motor activity recorded by prolonged radiotelemetry.

In order to characterise human interdigestive cyclical motor activity, and its interruption by food, jejunal pressure changes in healthy volunteers were recorded continuously for 24 hours, using an ingested pressure-sensitive radiotelemetry capsule tethered at the duodenojejunal flexure. In 20 studies, the subjects fasted throughout; in another 20 studies they received a single standard meal. Using this technique, fasting motor complexes were easily detected. There was considerable variation in interdigestive cycle duration and in the interruption caused by food. The data were not normally distributed. The study indicates that any descriptions of 'atypical' jejunal motility patterns must take into account the wide variations seen in health, before they can be regarded as representing dysfunction or disease.

Adult↗
PubMed1978

Purification and Characterization of a Cation-stimulated Adenosine Triphosphatase from Corn Roots.

A membrane-bound, monovalent cation-stimulated ATPase from Zea mays roots has been purified to a single band on sodium dodecyl sulfate gel electrophoresis. Microsomal preparations with K(+) -stimulated ATPase activity were extracted with 1 m NaClO(4), and the solubilized enzyme was purified by chromatography on columns of n-hexyl-Sepharose, DEAE-cellulose, and Sephadex G-100 Superfine. A 500-fold purification over the activity present in the microsomes was obtained. The K(+) -stimulated activity shows positive cooperativity with increasing KCl concentrations. The purified enzyme shows K(+) -stimulated activity with ATP, GTP, UTP, CTP, ADP, alpha + beta-glycerophosphate, p-nitrophenyl phosphate, and pyrophosphate as substrates. Under most conditions ATP is the best substrate. Although dicyclohexyl carbodiimide and Ca(2+) inhibit and alkylguanidines stimulate the K(+) -ATPase while bound to microsomes, they have no effect on the purified enzyme.

Journal Article↗
PubMedDate not supplied

Brain calcium in the rabbit: site of action for the alteration of body temperature.

Body temperature was recorded in the unanesthetized rabbit during push-pull perfusion of regions of the hypothalamus. Both the anterior and posterior hypothalamus were perfused with physiological solutions containing Ca++ in a concentration 2.0 or 5.0 times that of extracellular fluid. The animals were placed in an ambient temperature of 4.0 +/- 2.0degrees C for at least 1 hr before the perfusion and all experiments were carried out at this temperature. In the posterior hypothalamic area Ca++ produced a sharp fall in body temperature but did not cause body temperature to alter when it was perfused through the anterior hypothalamic area. These results indicate that the rabbit is similar to the cat and monkey since the effect of Ca++ on body temperature is localized to the posterior hypothalamic region.

Animals↗