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Biomedical subjects

M J Corbel

Publications and source records attributed to M J Corbel.

At least 73 records · Page 4Linked to original sources
PubMed1987

Experimental Brucella abortus infection in pigs.

Sixteen pigs inoculated by the intra-conjunctival, intravenous or subcutaneous routes with Brucella abortus Strain 544 developed a short-lived infection usually accompanied by conjunctival and vaginal excretion of the organism for up to 99 days post-inoculation. Serological tests performed by the agglutination, complement fixation, Rose Bengal plate, antiglobulin, immunodiffusion or ELISA procedures with B. abortus antigens disclosed wide variations in the antibody responses of individual animals. In some cases the serological tests were negative even though the animal was shown to be excreting B. abortus. The intradermal test for delayed hypersensitivity to Brucella antigens gave more consistent results, especially when supported by histological evaluation of the skin reactions.

Animals↗
PubMed1987

Lectin agglutination of thermophilic Campylobacter species.

Agglutination tests with lectins indicated differences in the surface composition of strains of the thermophilic (optimum temperature 42 degrees C) Campylobacter species C. coli, C. faecalis, C. hyointestinalis, C. jejuni and C. laridis. All strains examined were agglutinated by the protein-reactive agglutinins of Mangifera indica (mango) and Persea americana (avocado) and a large proportion was also agglutinated by the carbohydrate-reactive lectins of Canavalia ensiformis (Jack bean) and Triticum vulgaris (wheat germ). Reactions with other lectins varied widely between strains, even of the same species and serotype. Lectin agglutination may be useful as a supplementary procedure for characterizing individual Campylobacter isolates for epidemiological purposes.

Agglutination Tests↗
PubMed1985

Development of improved methods for the transport and isolation of Haemophilus somnus.

The survival of several strains of Haemophilus somnus under various simulated transport conditions was investigated. Recovery of H somnus from alginate swabs kept at room temperature was possible for up to 27 hours after sampling, but this period could be extended to 72 hours if the swabs were refrigerated. Storage of swabs in transport media did not prolong survival time significantly but did increase the number of bacterial contaminants, thus making recovery of H somnus less likely. The sensitivity of several strains of H somnus to a number of dyes, antibiotics and other antimicrobial agents was determined and a selective isolation medium then formulated. This medium which consisted of brain heart infusion agar supplemented with 5 per cent ovine blood, 5 per cent equine serum, 0.5 per cent yeast extract, cycloheximide (100 micrograms ml-1) and lincomycin (3 micrograms ml-1), facilitated the isolation of H somnus from contaminated material. However, while this medium was effective against many contaminants, it did not prevent swarming by Proteus species.

Anti-Bacterial Agents↗
PubMed1985

Urease activity of Brucella species.

Examination of the urease activity of 604 brucella strains showed a limited correlation with species. Most strains of B canis, B neotomae and B suis gave a positive urease reaction within 15 minutes, although some exceptions were noted. A substantial proportion of strains of B abortus and B melitensis also hydrolysed urea as rapidly as most B suis strains. Although most B ovis strains were negative to the urease test, 28.9 per cent of those examined gave positive reactions.

Brucella↗
PubMed1984

The interaction of Brucella cell surface components with plant agglutinins.

Cells of all Brucella species were agglutinated by the basic protein-reactive lectins of Mangifera indica and Persea americana. Cells of non-smooth strains including B. ovis and B. canis but not smooth strains were also agglutinated by concanavalin A. These lectins also reacted in a similar pattern with lipopolysaccharide extracts of these organisms. The lectin from Bandeiraea simplicifolia precipitated with the lipopolysaccharide antigens of smooth Brucella organisms and with those of serologically cross-reactive strains of Escherichia coli, Pseudomonas maltophilia, Salmonella and Yersinia enterocolitica but not with antigenically unrelated strains.

Agglutination Tests↗
PubMed1984

Properties of an immunogenic fraction from Brucella abortus 45/20.

Hot saline extracts of Brucella abortus strain 45/20 contain up to 4 protein components. Some of these components are shared with other Brucella strains but one appears specific to B. abortus 45/20 or possibly other non-smooth Brucella strains. The extracts are antigenic in guinea-pigs and evoke protective immunity against virulent B. abortus. The rough antigen specific activity appears to be associated with a protein component rather than lipopolysaccharide.

Animals↗
PubMed1984

Properties of Brucella-phages lytic for non-smooth Brucella strains.

A series of host-range mutants has been selected for brucella-phage R. Two of these mutants designated R/O and R/C have been used for typing purposes. Phage R/O is lytic for non-smooth strains of Brucella abortus and for B. ovis. It is genetically unstable however and produces mutants lytic for smooth B. obortus and B. suis. Phage R/C is lytic for non-smooth B. abortus and for B. ovis and B. canis. It is much more stable than phages R or R/O and shows little or no lytic activity on smooth Brucella strains. It has been effective in differentiating B. canis from B. suis in tests on a limited number of strains. In their properties, all of the brucella-phages of the R series resemble their parent phage.

Adsorption↗
PubMed1984

Observations on serological cross-reactions between smooth Brucella species and organisms of other genera.

Serological cross-reactions have been demonstrated between smooth Brucella species and Escherichia coli 0:116 and 0:157, Salmonella serotypes of Kauffmann-White group N, Pseudomonas maltophilia and Yersinia enterocolitica 0:9 and 0:16. All of these organisms may evoke significant titres of cross-reacting antibodies in cattle and pigs inoculated by parenteral routes. E. coli 0:157, Salmonella urbana and Y. enterocolitica 0:9 evoked substantial titres of cross-reacting antibodies in cattle inoculated by the oral route with live organisms. Only Y. enterocolitica 0:9 was effective in evoking cross-reacting antibodies to Brucella in pigs inoculated by the same route. Differentiation of cross-reacting and homologous antibodies to Brucella was difficult and was not accomplished consistently by any single test.

Animals↗