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Biomedical subjects

M J Curtis

Publications and source records attributed to M J Curtis.

101 records · Page 6Linked to original sources
PubMed1977

The effect of Escherichia coli endotoxin on lysosomal enzymes in the domestic fowl.

During the 2 h following the injection of chickens (aged nine to 11 weeks) with endotoxin isolated from a pathogenic strain of Escherichia coli (O78) there was a transient rise in the activity of acid phosphatase, aryl sulphatase and beta-glucuronidase in the plasma, suggesting increased release of these enzymes from lysosomes. This was followed by a fall in their activity which may have been due largely to stabilisation of the lysosomal membrane brought about by increased secretion of adrenocortical hormones and/or accelerated removal of the enzymes from circulation by the reticuloendothelial system.

Acid Phosphatase↗
PubMed1977

The effect of Escherichia coli endotoxin on plasma histaminase activity in the domestic fowl and the involvement of caeruloplasmin.

The injection of four-to nine-week-old fowls with Escherichia coli O111:B4 endotoxin (0-1 and 1-0 mg/kg) produced a two to eight fold rise in the histaminase activity of the plasma 24 h afterwards. In some cases this increase was still detectable after 48 h. This activity was strongly correlated with the p-phenylenediamine oxidase activity of caeruloplasmin. Electrophoretic studies with 7-5 per cent polyacrylamide gels indicated that fowl caeruloplasmin also was histaminase and putrescinase activity and that the release of this protein from the liver by endotoxin is largely responsible for the increase in the activity of the plasma. In untreated fowls this activity was lower than published values for several mammals and does not explain the relative resistance of the fowl to the acute effects of endotoxins and large doses of histamine.

Amine Oxidase (Copper-Containing)↗
PubMed1975

The effects of Escherichia coli endotoxin on the concentrations of mineral elements in the plasma of the domestic fowl.

The intravenous injection of E coli endotoxin (serogroup 0111 : B4) into eight to nine week old disease-free fowls (0-025-3-0 mg/kg) produced successive falls in the plasma potassium and calcium levels during the 9 h following the injection. This change in the potassium concentration was accompanied by a slight rise in that of sodium, and in some cases a slight rise in that of sodium, and in some cases a slight reduction in the magnesium concentration was detected when that of calcium was reduced. No effect was observed 24 h after the injection. The metal-binding properties of endotoxin, its affinity for membranes and its effect on the secretion of adrenocortical hormones may be involved in the production of these responses. Their pathophysiological significance is also discussed.

Animals↗
PubMed1969

The action of dilute aqueous NN-dimethylhydrazine on bacterial cell walls.

1. Walls of certain Gram-positive bacteria dissolved on incubation with dilute aqueous NN-dimethylhydrazine in the presence of air, by a reaction that probably involves free radicals. 2. Under the conditions described, the soluble products from the peptidoglycan were almost all non-diffusible. After brief incubation of walls of some organisms with reagent, part of the peptidoglycan component was obtained as a high-molecular-weight gel, the viscosity of which was rapidly decreased by incubation with lysozyme. 3. The extent to which peptidoglycan dissolved varied with different organisms, depending possibly on the extent of cross-linking, but the nature of the bonds that were destroyed has not been established. 4. Teichoic acids and polysaccharides were solubilized by this treatment and could be isolated in high overall yield. 5. The procedure is valuable in the examination of the distribution of heteropolymers in walls, and has been used to show that the polysaccharide present in walls of Lactobacillus arabinosus 17-5 is phosphorylated and may account for 20% of the total phosphate of the wall.

Bacillus megaterium↗
PubMedDate not supplied

Tarsometatarsal joint injuries in the athlete.

This is a retrospective review of the presentation, diagnosis, treatment, and outcome of 19 patients who injured the tarsometatarsal joint of the foot during athletic activity. Diagnosis by clinical and radiographic examination was supplemented by stress fluoroscopy of the articulation under anesthesia. Injuries were classified as either a first- or second-degree sprain of the tarsometatarsal joint, a third-degree sprain (with diastasis between the metatarsals or cuneiforms), a fracture, or frank dislocation. Poor functional results were seen in those for whom diagnosis was delayed and for whom the injury was not treated adequately. Three patients were unable to return to sports, one of whom eventually required fusion of the tarsometatarsal joint. The third-degree sprains were indistinguishable from fracture and fracture-dislocations in that good results were not reliably obtained by nonoperative treatment, and both classes of injury seem to require open reduction and internal fixation for optimal return to function. The delay in return to full activity is a marker of the severity of this injury despite an often benign appearance on radiograph.

Adolescent↗