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Biomedical subjects

M J Lang

Publications and source records attributed to M J Lang.

13 recordsLinked to original sources
PubMed2001

[Medetomidine-butorphanol combination anesthesia in the dog].

The combination of Medetomidine and Butorphanol leads to a sufficient sedation with good analgesia and is thus superior to the application of Medetomidine only. By reducing the dosage of Medetomidine, reduced cardio-respiratory side-effects are to be expected. The comparative application of the two Butorphanol preparations Torbugesic (group T) and Morphasol (group M) did not show any differences concerning effect and tolerance. No statistically significant and clinically relevant differences between the two groups were observed for any of the parameters.

Analgesics, Opioid↗
PubMed2000

Expression of proliferating cell nuclear antigen (PCNA) in oral submucous fibrosis, oral epithelial hyperkeratosis and oral epithelial dysplasia in Taiwan.

To test whether the oral epithelia of oral submucous fibrosis (OSF), epithelial hyperkeratosis (EH) and epithelial dysplasia (ED) may have increased proliferative activity under the long-term exposure to areca quid ingredients and whether there is an increased expression of proliferating cell nuclear antigen (PCNA) in oral premalignant lesions with disease progression, we used an immunohistochemical technique with the mouse monoclonal antibody PC10 to investigate PCNA expression in histologic sections of OSF, EH, ED and normal oral mucosa (NOM). Positive PCNA staining was found mainly in basal and parabasal epithelial cells in all specimens of OSF, EH, ED and NOM. The mean PCNA labeling indices (LI) in NOM, OSF, EH and ED were 8.8+/-2.7%, 22.1+/-12.5%, 25.5+/-5. 2% and 44.9+/-15.4%, respectively. Significant differences in the PCNA LI were noted between NOM and OSF (P<0.01), EH (P<0.001) or ED (P<0.001), as well as between ED and OSF (P<0.001) or EH (P<0.01). The gradual increase of PCNA expression with the morphologic transformation of normal epithelial cells into dysplastic epithelial cells suggests that there is increased proliferative activity in oral premalignant lesions with disease progression. However, no significant correlation was found between PCNA LI in OSF epithelium and the clinicohistologic parameters of OSF. In addition, the mean PCNA LI of p53-positive OSF cases (23.7+/-12.0%) was very close to that of p53-negative OSF cases (23.9+/-13.1%), suggesting that there was no association between PCNA and p53 expression in OSF.

Adult↗
PubMed2000

Expression of p53 protein in oral submucous fibrosis, oral epithelial hyperkeratosis, and oral epithelial dysplasia.

BACKGROUND AND PURPOSE: In our previous study, positive p53 staining was observed in 47 of 81 (58%) cases of oral squamous cell carcinoma associated with areca quid (AQ) chewing and cigarette smoking. This study looked for expression of p53 protein in premalignant oral lesions in patients who chewed AQ or smoked cigarettes, or both. METHODS: Expression of p53 protein was examined in histologic sections of oral submucous fibrosis (OSF, n = 50), epithelial hyperkeratosis (EH, n = 10), epithelial dysplasia (ED, n = 10), and normal oral mucosa (NOM, n = 10) with antibodies against p53 protein using an immunoperoxidase technique. RESULTS: Positive p53 staining was observed in 30 (60%) OSF specimens, four (40%) EH specimens, seven (70%) ED specimens, and none of the NOM specimens. Only four (8%) of the OSF specimens and none of the EH specimens had more than 25% p53-positive keratinocytes. However, in four (40%) of the ED specimens, more than 50% of the keratinocytes were p53-positive. The degree of p53 staining increased with the morphologic transformation of normal-appearing epithelial cells into dysplastic epithelial cells. There was no significant correlation between expression of p53 in OSF epithelium and the clinicohistologic parameters of patients with OSF. CONCLUSIONS: These results demonstrate that p53 is often present in precancerous lesions of patients who chew AQ and smoke cigarettes. We suggest that p53 may play a role in dysplastic cell transformation in premalignant oral lesions.

Adult↗
PubMed1999

Improvement of EPA method 8330: complete separation using a two-phase approach.

Complete separation of the 14 nitroaromatic and nitramine analytes targeted in EPA Method 8330 was achieved using a 30 x 4.6 mm Bondesil CN guard column in series with a 250 x 4.6 mm Bondesil C18 column (5 microns particles). Consistent with Method 8330 specifications, the mobile phase in the separation was 1:1 methanol:water and the flow-rate was 1.5 ml min-1. The success of this two-phase system proved to be largely a consequence of the unique resolution afforded by the Bondesil C18 column: complete separation of the 14 explosives could not be achieved using other CN/C18 column combinations. Additionally, while Method 8330 calls for a 100-microliter injection loop, separation on the Bondesil CN/C18 system was possible only using a 20-microliter injection loop. The loss of resolution with larger injection volume appears to be a result of the injection solvent, methanol, modifying the composition of the mobile phase both in the CN guard column and in the initial portion of the C18 column. The current method nevertheless represents an improvement over Method 8330, which calls for both a screening and confirming analysis. By separating the 14 Method 8330 analytes in a single run, the two-phase approach can increase sample throughput by decreasing analysis times.

Aniline Compounds↗
PubMed1995

Energy transfer and trapping in photosystem I reaction centers from cyanobacteria.

A mutant strain of the cyanobacterium Synechocystis 6803, TolE4B, was constructed by genetic deletion of the protein that links phycobilisomes to thylakoid membranes and of the CP43 and CP47 proteins of photosystem II (PSII), leaving the photosystem I (PSI) center as the sole chromophore in the photosynthetic membranes. Both intact membrane and detergent-isolated samples of PSI were characterized by time-resolved and steady-state fluorescence methods. A decay component of approximately 25 ps dominates (99% of the amplitude) the fluorescence of the membrane sample. This result indicates that an intermediate lifetime is not associated with the intact membrane preparation and the charge separation in PSI is irreversible. The decay time of the detergent-isolated sample is similar. The 600-nm excited steady-state fluorescence spectrum displays a red fluorescence peak at approximately 703 nm at room temperature. The 450-nm excited steady-state fluorescence spectrum is dominated by a single peak around 700 nm without 680-nm "bulk" fluorescence. The experimental results were compared with several computer simulations. Assuming an antenna size of 130 chlorophyll molecules, an apparent charge separation time of approximately 1 ps is estimated. Alternatively, the kinetics could be modeled on the basis of a two-domain antenna for PSI, consistent with the available structural data, each containing approximately 65 chlorophyll a molecules. If excitation can migrate freely within each domain and communication between domains occurs only close to the reaction center, a charge separation time of 3-4 ps is obtained instead.

Cyanobacteria↗
PubMed1993

Human peritoneal mesothelial cells synthesize interleukin-6: induction by IL-1 beta and TNF alpha.

Recent studies have demonstrated increased levels of IL-6 in the peritoneal cavity during CAPD peritonitis. The current investigation was initiated (i) to examine the human peritoneal mesothelial cell (HPMC) as a possible source of this secreted IL-6 and (ii) to characterize the released product and examine its regulation by other cytokines. Unstimulated HPMC under growth arrested conditions released IL-6 in a time dependent manner. After 24-hour HPMC IL-6 release (mean +/- SEM, N = 13) (expressed as pg/micrograms cell protein) was 1.67 +/- 0.33. Stimulation of HPMC with IL-1 beta or TNF alpha resulted in a time (increasing up to 48 hr) and dose dependent IL-6 generation. After 24 hours the levels induced by IL-1 beta and TNF alpha (both at 1000 pg/ml) were (mean +/- SEM, N = 13) 19.08 +/- 2.98 and 6.62 +/- 1.72, respectively. Stimulation with combinations of IL-1 beta and TNF alpha resulted in additive increases in IL-6 release. This release could be inhibited by co-incubation with anti-IL-1 beta and/or anti-TNF alpha antibodies. The level of released HPMC IL-6 measured by immunometric assay (ELISA) correlated directly with that detected in the 7TD1 IL-6 bioassay (r = 0.63; P < 0.001). Western blot analysis of concentrated HPMC supernatants using specific anti-IL-6 antibody demonstrated immunoreactive bands at 23 and 28 Kd following IL-1 beta or TNF alpha treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

Base Sequence↗
PubMed1991

Dizygotic twins concordant for truncus arteriosus.

Persistent truncus arteriosus (TA) is an uncommon congenital cardiovascular malformation, which comprises between 0.4% and 4% of all congenital heart defects. Occurrence of TA in siblings has been reported infrequently. Twins concordant for isolated TA appear to have been reported only once previously. In this paper, we describe dizygotic twin females who were concordant for isolated TA.

Diseases in Twins↗
PubMed1990

A controlled trial of human surfactant replacement therapy for severe respiratory distress syndrome in very low birth weight infants.

In a randomized, controlled study, human surfactant derived from amniotic fluid was administered within 12 hours of birth to infants with severe respiratory distress syndrome who were born at 24 to 32 weeks of gestation weighing less than or equal to 1500 gm. A second dose of surfactant was given to patients in the treatment group if they met ventilator requirements indicating relapse or lack of response to the initial dose. No significant improvement was observed in mortality rate (9/28 vs 15/31) or incidence of bronchopulmonary dysplasia (5/28 vs 3/31) when surfactant-treated infants were compared with control subjects, although there was a significant reduction in initial respirator and inspired oxygen requirements and the arterial/alveolar oxygen ratio improved. In addition, there was a significant reduction in pulmonary air leak in treated infants (10/28 vs 20/31; p less than 0.05). Retreatment was associated with an attenuated ventilatory response and with a higher mortality rate (7/14) than that of infants who did not require a second dose (2/14; p = 0.05), indicating a more severe form of disease. Multiple discriminant analysis, including eight independent variables, revealed that increasing birth weight, earlier age at surfactant treatment, and female gender were significantly associated with survival. These data suggest that early surfactant treatment may reduce mortality rates in very low birth weight infants with severe respiratory distress syndrome, as well as reduce ventilator requirements and the incidence of pulmonary air leaks.

Age Factors↗
PubMed1989

Nonphenylketonuric hyperphenylalaninemia.

Sixteen subjects with nonphenylketonuric hyperphenylalaninemia were followed up during a period of years. Dietary treatment did not seem to influence the outcome, and no relationship between blood phenylalanine and intellectual outcome was demonstrable.

Adolescent↗
PubMed1988

[Expression of epithelial and nervous-system-related antigens on pleomorphic adenoma of the salivary glands--an analysis of 20 cases].

The expression of cytokeratin (40-52 kD), carcinoembryonic antigen (CEA), vimentin, neuron-specific enolase (NSE), S-100 protein, glial fibrillary acidic protein (GFAP) were investigated in 20 cases of pleomorphic adenoma of the salivary glands and 10 cases of normal salivary glands, in order to analyze and correlate the antigens' expressions with the probably histogenetic mechanisms of the various histopathological differentiations in pleomorphic adenoma of the salivary glands and their probably original cells in normal salivary glands. Immunohistochemistry has provided some evidence for the relationship of the tumor cells to normal salivary glands: In the normal glands, the acinic cells exhibited cytokeratin, CEA and focal, predominantly nuclear S-100 protein staining. In both normal glands and pleomorphic adenomas, the duct-lining cells were immunoreactive for cytokeratin, CEA and had both cytoplasmic and nuclear S-100 positivity; The myoepithelial cells of the normal glands as well as the periduct cells, epithelial nests/cords, squamous metaplasia and the stellate/spindle/cartilaginous cells in the myxomatous-chondroid areas of the pleomorphic adenoma contain immunoreactive vimentin, NSE, S-100 proteins and GFAP, and lesser amounts cytokeratin (40-52 kD)/CEA. The varicosities of the terminal axon may lie directly on the basal membrane, or penetrate the basal membrane and lie in direct contact with the effector cells (duct-acinar-myoepithelial cells) of the salivary glands. The peripheral neurons and axons of the autonomic nervous system were identified by vimentin, NSE, S-100 proteins and GFAP. The combination of epithelial cytokeratin and nervous system-related vimentin, NSE, S-100 and GFAP immunostaining in myoepithelium of the normal glands and in all component elements (particularly the periduct cells) of pleomorphic adenoma reflects pleomorphic adenoma of the salivary glands is an epithelial tumor, the probably original cells or the probably histogenetic mechanisms of the various histopathological differentiations is correlated not only with "duct-acinar-myoepithelial cells" but also with the neuroectoderm in the normal salivary glands."

Adenoma↗
PubMed1984

Developing and negotiating transfer agreements in allied health.

The Kentucky Council on Higher Education has developed a statewide articulated system for allied health education. The articulated system permits entry and exit of prepared professionals at a variety of levels. The six disciplinary clusters included in the system are clinical laboratory sciences, dental auxiliaries, dietetics/nutrition, rehabilitation therapies, radiological sciences, and respiratory therapy. Competency-based education was used as the theoretical framework for developing and negotiating transfer agreements. Types of agreements included the block credit transfer agreement and the course specific transfer agreement. Agreements were negotiated between education systems and between specific programs/institutions. To date, approximately 30 transfer agreements have been negotiated. The process for negotiating transfer agreements included the following sequence: identify programs, develop a theoretical articulation model, rank order transfer agreements, determine the type of agreement, review respective curricula, determine the existing amount of transferable coursework, draft a pre-articulation agreement, schedule a meeting between faculty and administrators to discuss the agreement, and secure authorizing signatures on the final transfer agreement. Facilitators and barriers to negotiating agreements are discussed and recommendations offered for others who may be interested in developing transfer agreements.

Allied Health Personnel↗
PubMed1984

Statewide articulation for allied health: a conceptual model in action.

Under the auspices of the Kentucky Council on Higher Education, and with the aim of delineating issues in allied health education and making recommendations for alleviating the issues, an indepth, two-year study was completed in 1975. The primary recommendations pertained to the development of a statewide plan for allied health education that would result in an articulated statewide system permitting entry and exit of prepared personnel at a variety of levels and improving inter-institutional efforts to share educational resources. In the following four years a foundation was laid for developing the system. An articulated system of allied health education was developed in six disciplinary clusters to serve as a model for other states: clinical laboratory, dental auxiliaries, dietetics/nutrition, rehabilitation therapies, radiological science, and respiratory therapy programs. All levels of programs within each discipline cluster existing in Kentucky are included in the system. The study includes a design phase and an implementation phase. During the design phase, six discipline advisory groups developed articulation models for the programs in each discipline. Implementation phase accomplishments include signed transfer agreements, development of model and core curricula, design for graduate follow-up, publications, development of manpower models, and increased cooperation among project participants.

Allied Health Personnel↗