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Biomedical subjects

M J Milano

Publications and source records attributed to M J Milano.

11 recordsLinked to original sources

Reality versus suggestion: pseudomemory in hypnotizable and simulating subjects.

Assigned hypnotizable (N = 56) and simulating Ss (N = 44) to 1 of 4 conditions: heard a phone ring and conversation, received a suggestion to hear a phone ring and conversation, received a suggestion and heard a phone ring and conversation, or neither heard a phone nor received a suggestion. Hypnotizable Ss successfully discriminated objective events from suggested sources of input. When Ss received a suggestion to hear a phone ring, only 11.5% indicated it actually rang in their open-ended reports; in response to a forced-choice question, none did so. In spontaneous reports, none of the hypnotizable Ss who heard a phone ring indicated it was suggested; only one did so in response to a forced-choice item (vs. 2 simulators). In the no-phone/no-suggestion condition, more simulators than hypnotizable Ss indicated that a phone rang or was suggested.

Auditory Perception↗

Applications of a Vidicon spectrometer to analytical problems in clinical chemistry.

This report discusses characteristics of a custom-designed vidicon spectrometer and evaluates its applicability to several clinical analysis problems. Data show that the vidicon detector response is linear with intensity over about four orders of magnitude and that the uncertainty in absorbance measurements can approach 0.001 absorbance units in the range from 0 to 2 absorbance units. Applications include the enzymatic determination of glucose, the determination of lactate dehydrogenase, and determinations of barbital, chlordiazepoxide, and glutethimide. Capabilities of the instrument system for first-derivative spectroscopy are also discussed. The discussion included a critical evaluation of the potential advantages and limitations of the concept.

Autoanalysis↗

Vidicon spectrometer applied to simultaneous enzyme determinations.

Lactate dehydrogenase and alkaline phosphatase activities in the same medium can be determined simulataneously, at 350 and 550 nm, with a vidicon spectrometer. Substrate concentrations and ph have been made optimum for the combined analysis. These conditions result in activities for lactate dehydrogenase that are equivalent to those found by methods in common use, and activites for alkaline phosphatase that are about 31% below the maximum values that could be obtained with its substrate used at the same ph and temperature in the absence of NAD+ and lactate. However, activites measured by the simultaneous analysis were proportional to those obtained by other methods used in clinical laboratories, and the coefficients of variation were 2.3% for lactate dehydrogenase and 3% for alkaline phosphatase.

Alkaline Phosphatase↗