Treatment of advanced cancer of head and neck by large doses of methotrexate.
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Administration, Oral↗
Biomedical subjects
M J Moriarty
Publications and source records attributed to M J Moriarty.
Glucose analogues alter the response of CHO-KI cells to gamma irradiation.
Pretreatment of CHO-KI cells with 2-deoxy-D-glucose or L-glucose, two glucose analogues, reduces their survival if subsequently exposed to 60Co irradiation. The reduction in survival is constant irrespective of the time the cells are in contact with the analogues before irradiation and occurred even when cells were irradiated 6 hours after plating, suggesting that cell cycle effects are probably not involved. Interestingly, split-dose recovery was not affected to the expected degree, despite a reduction in the extrapolation number of the primary curve. It is suggested that interference with energy production from glucose is responsible for the reduced capacity of cells to survive the irradiation.
Animals↗
Radiobiologic response of CHO-KI cells treated with vitamin A.
Treatment of CHO-KI cells with vitamin A altered their response to subsequent gamma irradiation. In general longterm preincubation with low doses of the vitamin caused a relative increase in the number of cells surviving a given radiation dose. The effect resulted in an increase in the D0 of the survival curve. Long or short term exposure to high concentrations of the vitamin caused a decrease in the number of surviving cells leading to a decrease in the extrapolation number of the survival curve. Recovery of cells from radiation damage, assessed using the split dose technique, was also impaired by vitamin A pretreatment. A mechanism involving repair of potentially lethal damage may explain the protective effect of vitamin A since this was highly dependent on the cell density of cultures at the time of irradiation. However, in view of the data showing that the vitamin A concentrations necessary to alter the radiation survival curve shoulder caused a significant release of sialic acid into the medium, a mechanism involving membrane stability may account for both the reduction in repair/recovery capacity of the treated cells and the radioprotective effect.
Animals↗