Biomedical subjects

M J da Costa

Publications and source records attributed to M J da Costa.

4 recordsLinked to original sources

PubMed1992

Effect of air temperature and humidity on ingestive behaviour of sheep.

Thirty-two Polwarth ewes, of ages up to 1 year, were observed in a climatic chamber (24 to 45 degrees C) for eight periods of 5 h each. The observations were made through a window in the chamber wall. All animals were observed four times, then shorn and observed four times again. The animals were given weighed quantities of water and feed consisting of commercial concentrate plus Rhodes grass (Chloris gayana) hay. The water and feed remaining after 5 h of observation were weighed. The following traits were analysed: time eating hay (TEH), time eating concentrate (TEC), time drinking water (TDW), weight of hay eaten (WHE), weight of concentrate eaten (WCE), volume of ingested water (VIW), ruminating time standing up (RTS), ruminating time lying down (RTL), idling time standing up (ITS), and idling time lying down (ITL). Shearing had a significant effect for all traits except ITS. Shearing resulted in higher values for all traits except for ITS and ITL. Ingestion of hay (TEH and WHE) decreased with increased air temperature and humidity, while the ingestion of concentrate (TEC) and WHE) and water (TDW and VIW) increased. Rumination decreased with increased air temperature and humidity, and was higher in shorn than in unshorn sheep.

Animals↗

PubMed1992

Influence of hot environments on some blood variables of sheep.

Thirty-two Polwarth sheep of ages up to 1 year were observed under temperatures varying from 10.5 to 46.5 degrees C. The following blood cell counts were made: erythrocyte (RBC), leucocyte (WBC), eosinophil (EOS), neutrophil (NEU), lymphocyte (LYM) and monocyte (MON). Other traits measured were: haemoglobin (HB), haematocrit (HT), blood glucose (GLU) and serum protein (PROT). Multivariate analysis of variance was used and the results showed a significant (P < 0.001) effect for the interaction of shearing and temperature treatment. Under temperatures > 25 degrees C, sheep presented a decrease of RBC, WBC, HB and HT, these differences being greater in the shorn than in the unshorn animals. Unshorn animals presented higher variations in EOS, NEU, LYM, MON and GLU. Blood glucose increased under high temperatures in the shorn animals (from 56.36 +/- 0.65 mg/100 ml to 60.52 +/- 0.69 mg/100 ml) as in the unshorn animals (from 54.72 +/- 0.74 mg/100 ml to 57.56 +/- 0.77 mg/100 ml).

Animals↗

PubMed1991

[Post-vaccination tuberculin response using PPD derived from BCG].

The aim of this study was to compare the biological response obtained with two tuberculin tests--PPD-RT 23 and PPD derived from BCG--among children already vaccinated with the Calmette-Guérin bacillus. Results varied widely, and the differences observed were statistically significant. This shows the need for additional studies to assess the sensitivity, specificity and predictive value of both tests.

BCG Vaccine↗

PubMedDate not supplied

Detection of N-myc amplification in neuroblastomas using Southern blotting on fine needle aspirates.

OBJECTIVE: To verify the possibility of detecting N-myc amplification in fine needle aspiration (FNA) cytology from neuroblastomas by the Southern blotting technique. STUDY DESIGN: Fifteen neuroblastomas diagnosed by FNA in the Department of Pathology, Hospital de S. João, between 1990 and 1998, were studied for N-myc amplification using the Southern blotting technique in cytologic and histologic material. RESULTS: DNA extraction from the cytologic material was performed in all cases (N = 15). In two cases the quality/quantity of the DNA did not allow the study of N-myc status by the Southern blotting technique. We detected N-myc amplification in 1 of 13 patients (7.6%) from whom material was available for genetic study. CONCLUSION: It is possible to use the Southern blotting technique to demonstrate N-myc amplification in material obtained from FNA of neuroblastomas.

Adrenal Gland Neoplasms↗