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Biomedical subjects

M Jílek

Publications and source records attributed to M Jílek.

At least 19 recordsLinked to original sources
PubMed1991

Class IgG, IgM and IgA antibodies against Staphylococcus aureus antigens in human serum and saliva.

Using the ELISA method antibodies against the sonicate, teichoic acid (TA) and exoproducts of Staphylococcus aureus were determined in sera and saliva of healthy individuals. Main serum antibodies against all the antigens used were shown to be class IgG antibodies. However, antigens of the sonicate stimulated significantly even the systemic IgA response. In the saliva class IgA antibodies predominated, but IgG antibody levels against TA and exoproducts approached the level of IgA antibodies. Levels of IgM antibodies against all antigens tested were low in both the serum and saliva which corresponds with the anamnestic type of response. On the basis of these results one may assume that not only IgG, but also IgA antibodies are important in the systemic immunity against staphylococcal infection and in the immunity of mucous membranes; besides IgA, even class IgG antibodies play an important role.

Adolescent↗
PubMed1989

The number of plaque-forming cells and its "normal range".

The number of antibody-forming cells is commonly assessed by the plaque technique. In this paper, two questions are discussed, connected with presenting the number of plaque-forming cells found in experiments: (i) the form of probability distribution of the number of plaque-forming cells (PFC), and (ii) determination and use of the so-called "normal range" of the number of PFC. Gottlieb's finding (1974) that the lognormal distribution is a good approximation to the distribution of the number of PFC is supported, and it is suggested that the upper one-sided tolerance limit be used as the limit of normal range of the number of PFC. A numerical study based on 1,450,000 pseudo-random experiments shows that it is important to make a correct assumption about the form of the distribution before a statistical method is used.

Animals↗
PubMed1986

A new mathematical model of proliferation control during immune response.

A considerable proliferation of participating cells is a characteristic feature of the immune response. This proliferation may be controlled by Interleukin 2. Assumptions on the course of the immune response under such a control are formulated, and a new mathematical model of the immune response involving regulation of the proliferation of appropriate cells is constructed.

Antibody Formation↗
PubMed1985

The X----Y----Z scheme after 23 years.

Mathematical modelling of the course of the immune response is undoubtedly one of the most progressive and most promising areas of modern immunology. Mathematical models (along with computer programs) can be taken as "the only means of thoroughly testing and examining a large and intricate theory" (Partridge et al. 1984). The first phase of construction of mathematical models is the formulation of assumptions based on the knowledge of the facts to be modelled (manifested usually in a scheme of the presumed course of the modelled process). The first mathematical models of immune response were based on the hypothesis of a two-stage differentiation of cells participating in the humoral response, published in Prague 23 years ago (Sercarz and Coons 1962; Sterzl 1962) and illustrated by the X----Y----Z scheme. Many contemporary mathematical models still stem from this scheme which undoubtedly fits the fundamental data concerning the immune system.

Animals↗
PubMed1975

An estimate of the number of cells arising by division in mouse cerebral hemispheres from age one to 12 months: an autoradiographic study of DNA synthesis.

Male CBA strain mice aged 1, 2, 4, 6, 8 and 12 months were injected with 5 muCi of 6-H3-thymidine per gm body weight and killed two hours after the injection. Incorporation of the isotope, as a measure of DNA synthesis and cell division, was studied in the cerebral cortex and corpus callosum by light-microscope autoradiography. DNA synthesis was found in a small number of nuclei of non-neuronal cells, both of ecto- and mesodermal origin. The percentage of labeled cells (Labeling Index, or LI) decreased in both regions exponentially with age. On the base of measured LI values, the total number of cells arising from age one to 12 months was calculated. As the reference value the number of cells in one month-old animals was taken. The calculated cell increment in the ectodermal population in the cortex amounted to 19.7-20.6% (average 20.2%) and to 196.0-425.0% (average 247.0%) in the corpus callosum (pairs of figures correspond to values calcualted from LI values measured in sagittal and frontal sections). When all labeled cells (LC) were taken into consideration (of both ecto- and mesodermal origin) the corresponding cell increment ranged from 25.0-30.5% (average 26.8%) in the cortex and from 205.0-454.8% (average 263.0%) in the corpus callosum. The number of all newly arising cells in the whole hemisphere (including both cerebral cortex and corpus calosum) calculated from mean LI values of all LC in sagittal and frontal sections amounted to 52.0%. It is suggested that the observed DNA synthesis and cell division, particularly in the corpus callosum and mesodermal cell groups of both regions studied, correspond mainly to renewal of cell populations. In the cerebral cortex accumulation of some newly arising cells in situ cannot be excluded.

Aging↗