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M Jíra

Publications and source records attributed to M Jíra.

47 records · Page 3Linked to original sources
PubMed1984

Further evidence of immunomodulatory effects of cimetidine.

The immunomodulatory effect of cimetidine was demonstrated both on human lymphocytes in vitro and in an experimental mice tumour model. Cimetidine increased the incorporation of tritiated thymidine into peripheral blood lymphocytes of healthy donors after nonspecific stimulation with PHA and Con A in vitro. Cimetidine prolonged the survival of mice bearing the syngeneic ascitic lymphoma EL-4. It had no cytotoxic effect on cells of ascitic lymphoma EL-4 in tissue culture.

Adjuvants, Immunologic↗
PubMed1983

Ultrastructural localization of DNA in tissue culture cell nuclei by means of enzyme-gold and autoimmune sera-protein A-gold techniques.

The post-embedding localization of DNA was investigated in cell nuclei by means of DNase I-gold and autoimmune sera-protein A-gold techniques. Using the former technique, gold particles were found mainly over euchromatin, nucleoli exhibit occasionally high labeling. In the latter technique, the use of the serum binding dsDNA confined the label mainly to condensed chromatin.

Actins↗
PubMed1981

Lymphocyte sensitization detected by the macrophage electrophoretic mobility assay in patients with renal cell carcinoma: Theophylline increases the sensitivity of the assay.

An antigen-induced release of a macrophage slowing factor (MSF) by peripheral blood lymphocytes was used to evaluate lymphocyte sensitization to various antigens in 30 patients with renal cell carcinoma (RCC) and in 14 normal individuals. Twenty-three of 30 (77%) patients with RCC, but no healthy controls were found to be sensitized to a soluble antigen prepared from an allogeneic kidney tumor by 3 M potassium chloride extraction. Peripheral blood lymphocytes from some patients with RCC displayed sensitization to protein isolated from fetal kidney (6 of 24; 25%), control "normal" kidney (6 of 30; 20%) and urinary bladder carcinoma (3 of 21; 14%) tissues. It has been suggested that cyclic adenosine 3' ,5'-monophosphate (cyclic AMP) could play a role in the mechanism of the MSF action (24). In agreement with this idea, the presence of 10-4 M theophylline enhanced the macrophage electrophoretic mobility (MEM) reduction caused by MSF. Furthermore, the DNA synthesis in lymphocytes (monitored by measuring the uptake of tritiated thymidine) on contact with phytohemagglutinin (PHA) was depressed in 9 of 21 (43%) patients with RCC as compared with healthy controls.

Adenocarcinoma↗