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Biomedical subjects

M Jandoseková

Publications and source records attributed to M Jandoseková.

4 recordsLinked to original sources
PubMed1999

Anoxia/induced membrane changes in human red blood cells.

The cation-osmotic hemolysis was studied in human red blood cells incubated under anoxic conditions. In relation to the time course of anoxia, two phases of hemolysis were distinguished. A significant decrease of hemolysis was found between 3 and 24 h of incubation. On the other hand, hemolysis was significantly increased after prolonged incubation (48-72 h). Using the method of cation-osmotic hemolysis, the properties of two membrane constituents, spectrine membrane skeleton and membrane bilayer, were studied. The relation between cation-osmotic hemolysis and erythrocyte deformability is being discussed.

Erythrocyte Membrane↗
PubMed1999

Differences between cation-osmotic hemolysis and filterability in exaprolol- and glutaraldehyde-treated human red blood cells.

The changes in human red blood cell microrheology in different glutaraldehyde (3.0 and 5.0 x 10(-6) mol x l(-1)) and exaprolol (2.5 and 5.0 x 10(-4) mol x l(-1)) concentrations were studied. The method of millipore filtration was compared with the method of cation-osmotic hemolysis. Both drugs prolonged the filtration time. Cation-osmotic hemolysis in glutaraldehyde-treated cells was significantly lower in comparison with the control group. On the other hand, there was a significant increase in cation-osmotic hemolysis in exaprolol-treated cells. Besides cation-osmotic hemolysis and filterability of erythrocytes, we evaluated the medium cell volume (MCV) and the medium cell hemoglobin concentration (MCHC). No changes in MCV and MCHC in glutaraldehyde-treated cells were observed. However, the MCV was significantly lower and the MCHC was significantly higher in exaprolol-treated cells. In conclusion, we suggest that the method of cation-osmotic hemolysis is more sensitive than the filtration method for determination of red blood cell microrheology.

Adrenergic beta-Antagonists↗
PubMedDate not supplied

Comparison of two methods in erythrocyte microrheology determination using glutaraldehyde-treated cells.

The change in human red blood cell microrheology in different glutaraldehyde concentrations (1.5, 3.0 and 5.0 x 10(-6) mol l-1) was studied. The method of millipore filtration was compared with the method of cation-osmotic hemolysis. The obtained results revealed that the prolongation of the erythrocyte filtration time correlated with the curve shifts found in cation-osmotic hemolysis. Contrary to the filtration method, significant differences between two lower concentrations of glutaraldehyde (1.5 and 3.0 x 10(-6) mol l-1) were found. Therefore, we conclude that the cation-osmotic hemolysis is more sensitive than the filtration method in determining the red blood cell deformability.

Adult↗