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M Jullien

Publications and source records attributed to M Jullien.

49 records · Page 3Linked to original sources
PubMed1975

[Quasi-elastic light scattering. Application to biological problems].

The origin of the light scattered from macromolecular solutions is considered and a discussion is made around the use of laser and light beating spectroscopy to analyze the scattered field spectrum. Several experiments are reviewed where quasi elastic light scattering has been applied.

Actins↗
PubMedDate not supplied

Study of hydrophobic interactions between acylated proteins and phospholipid bilayers using BIACORE.

Intracellular proteins of eukaryotic cells are frequently covalently modified by the addition of long chain fatty acids. These modifications are thought to allow otherwise soluble proteins to associate with membranes by lipid-lipid based hydrophobic interactions. The purpose of this work was to quantify the effect of acyl chain length on hydrophobic interactions between acylated proteins and phospholipid monolayers. The binding of an artificially acylated model protein to electrically neutral phospholipids was studied by surface plasmon resonance, using BIACORE. Kinetic rates for the binding of bovine pancreatic ribonuclease A (RNase A), monoacylated on its N-terminal lysine with fatty acids of 10, 12, 14, 16 or 18 carbon atoms, to phospholipids on hydrophobic sensor chips, were measured. Unlike unmodified ribonuclease, acylated RNase A bound to the phospholipids, and the association level increased with the acyl chain length to reach a maximum for C16. Reproducible kinetics were obtained which did not fit a 1:1 Langmuir model but rather a two-step binding profile.

Acylation↗
PubMedDate not supplied

[Needle puncture biopsy: the contribution of extemporaneous examination of samples in the diagnosis of malignant liver tumors].

In this retrospective study, the authors compared the sensitivity and specificity, in terms of the diagnosis of malignancy, of two techniques of ultrasound-guided biopsy of liver tumours. The first technique involving fine needle biopsy with cytological and histological analysis of the specimen was carried out in 39 patients. A technique using a wide calibre needle with frozen section examination of the fragment obtained was performed in 27 patients. All patients had one or more malignant liver tumours. Sensitivity and specificity of the technique with frozen section histology were both 100 per cent, while figures for the technique with delayed histological study were 84.6 and 87.4 per cent. This study suggests that frozen section histology might be associated with greater sensitivity than delayed histology.

Adenocarcinoma↗