Biomedical subjects

M Jurkiewicz

Publications and source records attributed to M Jurkiewicz.

15 recordsLinked to original sources

PubMed2001

A potential double role of anti-Lewis X antibodies in Helicobacter pylori-associated gastroduodenal diseases.

In this study, we found Lewis X (Le(x)) determinants on 68% of Helicobacter pylori isolates from patients with chronic gastroduodenal diseases. Anti-Le(x) IgG were detected more frequently in the sera from dyspeptic children and adults (45 and 46%), with or without proved (culture) H. pylori infection, than in the sera from healthy individuals (14% and 25%). In contrast, the prevalence of anti-Le(x) IgM was higher in the groups of healthy individuals than in the groups of dyspeptic patients. Moreover, anti-Le(x) monoclonal antibody of IgM class enhanced the uptake of Le(x)(+) but not Le(x)(-) H. pylori isolates by phagocytes. In the sera from some dyspeptic patients, we detected Le(x)-anti-Le(x) IgG immune complexes (Le(x) ICs). There was a great difference between children and adults as regards the presence of Le(x) ICs. The immune complexes were found in the sera from nine out of 29 (27%) H. pylori-infected and three out of eight (37%) uninfected adult dyspeptic patients. In comparison, Le(x)-anti-Le(x) IgG ICs were detected only for two out of 18 (11%) H. pylori-infected children. Le(x) ICs were not found in the sera from healthy individuals. Our results suggest that anti-Le(x) IgM may play a protective role in H. pylori infections. In contrast, anti-Le(x) IgG and particularly Le(x)-anti-Le(x) IgG ICs might contribute to the pathogenesis of chronic H. pylori infections.

Adolescent↗

PubMed2000

[Chemical substances used in plastics intended for manufacture of food packaging in light of European Union legislation].

In the EU countries the use of the chemical substances for plastics intended to come into contact with foodstuffs is regulated by Directive 89/109/EEC and the several specific Directives. Positive lists of the chemical substances for plastics intended to manufacturing of food packaging are placed in Directive 90/128/EEC on plastics materials. This 90/128/EEC Directive so-called "Monomer Directive" has been amended five times by Directives 92/39/EEC, 93/9/EEC, 95/3/EC, 96/11/EC and 99/91/EC. Directive 90/128/EEC so-called "Monomer Directive" provides: list of monomers and other starting substances that can be used in the manufacture of plastics materials and articles, placed in two Sections (A and B), and list of additives initiated from Directive 95/3/EC. Other additives will be added to this list on the basis of their positive national regulatory status in the EU countries and limits of migration (global, specific). According to the EU legislation all the substances before placing on the positive lists are the evaluated and authorized by the scientific bodies (SCF and/or JECFA). The EU legislation on the plastics materials and articles intended to come into contact with foodstuffs will be implemented into Polish law. However all the regulations relating to the chemical substances and limits of migration (global and specific) are already accepted by the National Institute of Hygiene.

European Union↗

PubMed2000

[Production of reactive nitrogen and oxygen intermediates in human granulocytes and monocytes during internalization of live BCG bacilli].

The production of nitric oxide (NO) and reactive oxygen intermediates in granulocytes and macrophages from healthy volunteers, infected in vitro with live Bacille Calmette-Guerin (BCG) mycobacteria, was estimated. Significant differences in the biochemical reactions induced by BCG bacilli in granulocytes and monocytes are described. The activity of phagocytes was also investigated in the cultures with cytokines: IFN-gamma, TNF-alpha, GM-CSF, IL-4.

Cells, Cultured↗

PubMed1999

[Health quality criteria and the analytical procedures for the evaluation of pacifiers].

The health quality criteria for the evaluation of soothers for babies and young children have been developed and the analytical procedures were collected and indicated for the evaluation purposes. They include: sensory analysis global migration migration of metals (Sb, As, Ba, Cd, Pb, Cr, Hg, Se) to 0.07 M HCl analysis of volatile compounds chemical demand for oxygen presence of reaction accelerators presence of antioxidants The requirements concerning mechanical and chemical parameters and also general safety of the product have been developed and the procedures for the conformity checking of these parameters, based on Polish Standard and the draft project of European Standard EN 14000 series concerning soothers for babies and small children have also been presented. These criteria have been accepted by the Ministry of Health and Social Welfare as suitable for issuing of health certificates for placing on the market as well as for the current sanitary surveillance of soothers taken from the market. The criteria will be obligatory until the EN 1400 Standards come into force being introduced into Polish legislation.

Humans↗

PubMed1999

Anti-Lewis X IgM and IgG in H. pylori infections in children and adults.

A role of autoimmune processes in the pathology of Helicobacter pylori infections has been suggested. The Lewis determinants present in LPS molecule of H. pylori bacteria have been indicated as the cause of antigenic mimicry. In this study, the prevalence of IgM and IgG antibodies to Lewis X antigen in the sera from children and adults, with or without dyspepsia, infected or not infected with H. pylori, seropositive and seronegative for anti-H. pylori IgG were determined immuno-enzymatically (ELISA). Our results revealed that humans may produce anti-Lewis X antibodies, particularly of IgM class, in the absence of H. pylori infection or H. pylori independent dyspepsia. The production of such antibodies, by healthy children who had never been infected with H. pylori suggested that anti-Lewis X antibodies may occur naturally.

Adolescent↗

PubMed1998

Development of a biparametric bioanalyser for creatinine and urea. Validation of the determination of biochemical parameters associated with hemodialysis.

The construction and evaluation of an automated urea and creatinine biparametric biosystem using flow injection analysis (FIA) are described. The biosystem uses enzyme reactions that hydrolyse urea and creatinine producing ammonium ions. The enzymes used were creatinine deiminase and urease, which are immobilized covalently in flow reactors. The reactor with creatinine deiminase has the enzyme immobilized on controlled-pore glass beads, whereas urease is immobilized on a nylon open tubular reactor. Detection is realised with a flow-through ammonium ion-selective electrode with an inner solid-state contact (graphite-epoxy composite). Ammonium ions are separated from alkali ion interferents through a gas-diffusion cell. The bioanalyser is fully automated using software and electronics developed ex profeso in our laboratories. The analyser was validated off-line by measuring urea and creatinine from discrete effluent samples from hemodialysis equipment. Results agreed with concurrent analyses realised using hospital laboratory methods. There were no significant differences between the two sets of results at the 95% confidence level. Finally, the biparametric bioanalyser was validated on-line by measuring creatinine and urea levels in artificial kidney effluents. These measurements were useful in the determination of key biochemical parameters of clinical interest such as the mass of urea and creatinine extracted from the patient as well as the initial concentration of creatinine and urea in blood plasma. When the results of the bioanalyser were compared with those yielded by the usual methods, they showed no significant differences at the 95% confidence level when determining the mass of the analytes extracted by the hemodialyser or when determining the urea concentration in blood plasma. However, when measuring the creatinine concentration in blood plasma using the developed bioanalyser, significant differences appeared.

Biological Assay↗

PubMed1998

[Hygienic evaluation and analytical methods for the silicon rubber rubber intended to come into contact with food. Part I. Determination of the migration of the soluble substances into the aqueous model fluids].

The review of the regulations for the silicon rubber intended to come into contact with food in some European countries and USA was performed. On the basis of these regulations the principles of the hygienic evaluation and the analytical models for the testing of silicon rubber and elastomers contacting with food in Poland was presented. The testing include: sensoric analysis; migration of the soluble substances into aqueous model fluids; volatile substances; hardeners; catalyzers; migration of certain heavy metals; N-nitrosamines and N-nitrosatables. Some of these methods have been published in Polish Standards or in the Methodical Publications of the National Institute of Hygiene.

Catalysis↗

PubMed1998

[A review of the European community directives relating to materials and articles intended to come into contact with food products in the light of Polish legislation].

The integration of Poland with the European Community makes the harmonization of Polish legislation necessary. Among numerous Directives, which provisions should be introduced into domestic legislation before Poland enters the European Union, the Directives related to materials and articles intended to contact with food consist a large group. The review and analysis of 17 Directives that appeared up to the end of year 1997 have been done. The list of these Directives is shown in the Table I. The framework Directive 89/109/EEC on the approximation of the laws of the Member States relating to materials and articles intended to come into contact with foodstuffs have been comprehensively discussed together with the specific Directives concerning the following matters: symbol which should accompany the materials and articles intendet to contact with food: Directive 80/590/EEC; plastics materials: Directive 90/128/EEC with later ammendments provided by the Directives: 90/128/EEC, 93/9/EEC, 95/3/EC and 96/11/EC; vinyl chloride monomer (acceptable limit and analytical method): Directives: 78/142/EEC, 80/766/EEC and 81/432/EEC, regenerated cellulose film-Directives: 93/10/ECC i 93/111/EEC; ceramic articles: Directive 84/500/EEC; testing migration of constituents of plastic materials-Directives: 85/572/EEC and 82/711/EEC with later ammendments: Directives: 93/8/EEC, 97/48/EC, The relevant Polish regulatory provisions concerning the matters related to materials and articles intendet to contact with foodstuffs have also been discussed. The need for harmonization of Polish regulations with the relevant legislation adopted by the Member States of the European Community was emphasized.

Humans↗

PubMed1996

Validation of an automatic urea analyser used in the continuous monitoring of hemodialysis parameters.

The validation of an automatic urea analyser used in the monitoring of hemodialysis processes is reported. The analyser can indirectly determine dialysis parameters as dialysis delivery (KT/V) and protein catabolism (PCRn). These parameters are useful for the prescription and optimization of hemodialysis. The analyser, based on a previously-reported flow-injection analytical biosystem, was connected on-line to the effluent of a dialysis machine during several hemodialysis sessions. The urea concentration data were continuously processed and dialysis parameters were obtained in quasi real time by means of the integration of an adjusted time-dependent exponential function. These values were compared with those obtained by applying the methods traditionally employed in hospital laboratories. The evaluation comprised 24 data sets from several patients of different gender and age. No significant differences were found between the KT/V and PCRn results obtained with the usual method and those results produced by the analyser proposed here.

Humans↗

PubMed1996

[A method of determining global migration from plastic packing materials into model fluids simulating food products containing fat according to directives of the EWG Commission].

A method is described for determination of global migration into model fluids simulating food products with fat of isooctane, 50% and 96% ethanol from 33 samples of foils and laminates used for food packing. Bag, chamber, immersion and padding methods were used. Most samples complied with the requirements of the EEC Directives and the used method is simple and convenient. It should be introduced promptly for routine control of packing materials in the laboratories of the Province Sanitary-Epidemiological Stations.

Ethanol↗

PubMed1995

[A method for determining global migration into model fluids from plastic packaging materials, in accordance with recommendations of the EWG commission].

Samples of polypropylene, polyethylene, polystyrene foils, softened polyvinyl chloride foils and laminates, in which polyethylene or EVA copolymer or SULRYN ionomer formed the inner layer were subjected to tests for determination of the global migration to aqueous fluids using model methods: chamber test, bag test, immersion test and lining test. The tested materials comprised 37 samples of packaging materials. The tested samples were not exceeding the range of permitted global migration into aqueous model fluids as recommended by the EEC directives. The obtained results fell significantly below the accepted limit of 10 mg/dm2.

Food Contamination↗

PubMed1994

[Formaldehyde determination in packing paper for food products by the colorimetric method with acetylacetone].

Papers used for food packing were studied for formaldehyde migration from them by the colorimetric method with acetylacetone. The studied material comprised 14 samples of paper of Polish or foreign production: pergamenous paper, offset and sulphite papers and one sample from waste paper imported from Germany. Formaldehyde migration from these samples differed but never exceeded 1 mg/dm2, with the exception of the recycled paper from which it was 1.77 mg/dm2.

Colorimetry↗

PubMed1993

[Determination of formaldehyde content in artificial cellulose casings by the colorimetric method with acetylacetone].

Artificial cellulose casings destined for production of processed pork were studied for their ability of formaldehyde release by a colorimetric method with acetylacetone. The studied material comprised 24 samples of colourless imported cellulose casings. The migration of formaldehyde into distilled water under conditions laid down in the BGA regulations (Empfehlung XLIV) was not exceeding 0.5 mg/dm3.

Animals↗

PubMed1993

[Determination of unbound formaldehyde in protein coverings using the colorimetric method with chromotropic acid].

In the period 1989-1991 in a control of health quality of packing material and utility objects in contact with food 1964 samples of protein coverings for processed food were analysed, determining the content of formaldehyde, lead, arsenic and copper according to the requirements of Production Standard ZN-76/MPSS-M-19-149. It was found that in about 40% of covering samples formaldehyde level exceeded the acceptable level of 1000 mg/kg. In about 90% of the samples the level of formaldehyde was below 1800 mg/kg. In 14% of the samples the lead level was increased. Arsenic and copper were within normal range in most analysed coverings.

Colorimetry↗

PubMed1990

[Examination of plastic food-packaging materials].

The aim of the work was evaluation of the hygienic quality of plastic packaging material and its elements intended for direct contact with food products. The study carried out in the years 1986-1989 comprised 3066 samples taken from 20 provinces. A considerable number of the studied materials had no attest of the State Institute of Hygiene (19.54%). The proportion of samples with such attest accepted as unsuitable was lower than that without attest (unacceptable organoleptic features--4.83%, or results of chemical investigations--2.87%). In some materials high amounts of cadmium and lead were found which suggested that they had been made of technical plastic materials containing unacceptable stabilizers or dyes derived from these metals. The obtained results indicate the necessity of control of plastic materials intended for contact with food products to eliminate these which would have a negative effects on the health.

Cadmium↗