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M KAWAKAMI

Publications and source records attributed to M KAWAKAMI.

At least 19 recordsLinked to original sources
PubMed1965

EXPERIMENTAL SALMONELLOSIS. IV. LIPID CONTENT OF TOXIN L OBTAINED FROM SALMONELLA ENTERITIDIS.

Kawakami, Masaya (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Experimental salmonellosis. IV. Lipid content of toxin L obtained from Salmonella enteritidis. J. Bacteriol. 89:193-197. 1965.-An improved method is described for the chromatographic purification of toxin L from virulent Salmonella enteritidis. Toxin L is heat-labile, and its toxicity is reduced by trypsin and by phospholipase A treatment. Chemical studies of chromatographically and ultracentrifugally homogeneous toxin preparations show that toxin L is a phosphorus-containing protein. The infrared-absorption spectrum of a petroleum ether extract of toxin L hydrolysate indicates that this material possesses a lipid component.

Antitoxins↗
PubMed1963

EXPERIMENTAL SALMONELLOSIS. III. NEW TOXIC FRACTION (L) OBTAINED FROM SALMONELLA ENTERITIDIS AND ITS IMMUNOLOGICAL PROPERTIES.

Kawakami, Masaya (Gunma University, Maebashi, Japan), Nobutaka Osawa, and Susumu Mitsuhashi. Experimental salmonellosis. III. New toxic fraction (L) obtained from Salmonella enteritidis and its immunological properties. J. Bacteriol. 86:872-879. 1963.-A method is described for the purification of the heat-labile toxins of a fully virulent strain, 116-54, of Salmonella enteritidis by ion-exchange chromatography. One component of the heat-labile toxin (L) was homogeneous, as evidenced by the results of the ultracentrifugal analysis and agar gel diffusion test. The mouse ld(50) was 1.3 mug, and chemical studies indicated that this toxin was a simple protein in nature. It was also evidenced by chemical and immunological tests that this toxin differs from the O antigen (lipopolysaccharide-protein complex).

Animals↗