Biomedical subjects

M Kałowski

Publications and source records attributed to M Kałowski.

At least 19 recordsLinked to original sources

PubMed1993

[Study of obligate anaerobic bacterial sensitivity to tinidazole and metronidazole (determination of minimal inhibiting concentration--MIC)].

Comparative susceptibility testing of 428 strains of obligate anaerobic bacteria belonging to genera Propionibacterium, Arachnia, Actinomyces, Bacteroides, Prevotella, Porphyromonas, Anaerorhabdus, Fibrobacter, Fusobacterium, Peptostreptococcus and Clostridium to metronidazole and tinidazole was performed. The study of the susceptibility of anaerobic bacteria was carried out by the method of serial dilution in Brucella agar according to Finegold and Sutter (1972). Strains of B. fragilis species, B. fragilis group, other Bacteroides spp.. Fusobacterium spp. and Clostridium spp. were susceptible to both chemotherapeutics in clinically attainable concentrations. Of particular significance is the greater susceptibility of these bacteria to tinidazole. Taking into account this activity, tinidazole is a superior agent in the treatment of anaerobic infections. Both chemotherapeutics were not active against, rarely isolated from severe infections. Gram-positive anaerobic rods from genera Propionibacterium. Actinomyces and Arachnia and were partially active against peptostreptococci.

Bacteria, Anaerobic↗

PubMed1992

[Rapid preliminary diagnosis of anaerobic bacterial infections. I. Natural fluorescence of pus, purulent fluids and dressing under UV radiation].

For evaluation of usefulness of natural fluorescence of clinical materials in UV radiation as rapid diagnostic method of infections with anaerobes, 405 samples of pus, bloody-purulent fluids, blood, wound secretions, dressings and other materials were investigated. Occurrence of red-brick UV fluorescence of clinical materials was compared with results of culture aimed at isolation of non-sporeforming anaerobes from "B. melaninogenicus group (P. melaninogenica, P. intermedia and P. saccharolytics). Significant correlation red-brick fluorescence of clinical materials resulting from UV irradiation with presence in these materials of anaerobes such as P. melaninogenica, P. intermedia and P. asaccharolytics was detected. Investigation of clinical materials with application of fluorescence in UV radiation lasts only 1-2 minutes and together with preparation and microscopical inspection which is Gram-stained--only 15-20 min. Positive results of this test may constitute a basis for rapid, preliminary identification of the etiologic factor and for direction of chemotherapy in the early period of infection.

Bacteria, Anaerobic↗

PubMed1992

[Rapid preliminary diagnosis of anaerobic bacterial infections. II. Investigation of the presence of volatile and non-volatile fatty acids (C1-C6) in pus, purulent fluids and dressing by application of gas chromatography].

Studies were performed in the years 1986-1990 and dealing with 405 clinical materials originating from infections within the abdominal cavity, maxilla, reproductive organ, post-operative wounds as well as dressings, extremities, blood, bile and other types of material and usefulness of analysis by gas chromatography of presence of lower fatty acids in materials for rapid and preliminary diagnostics with strictly anaerobic bacteria was confirmed. Presence in chromatograms of isobutyric, butyric isovaleric, valeric, isocaproic and caproic acid, and partly of propionic acid, is a good indication of infection with strictly anaerobic bacteria. Moreover, presence of succinic acid may suggest presence of anaerobic infection, similarly as presence in chromatograms of three or more various lower fatty acids. Rapid, approximative diagnosis of anaerobic bacteria by application of gas chromatography is worth of broader application. Investigation of clinical material by this method lasts only 1-2 hours and obtained results my present a basis for preliminary diagnosis of the etiological factor and for direction of chemotherapy, which is very important in most early period of treatment of infections.

Bacteria, Anaerobic↗

PubMed1988

[Sterinole and cetylpirydine chloride influence on non-sporulated anaerobic bacteria of oral cavity].

Sensibility (MBC--Minimal Bactericidal Concentration) of 97 strains of non-sporulated anaerobic bacteria separated from clinical material from oral cavity to Sterinole (Polfa) and cetylpirydine chloride has been examined. Experiments have been carried out by means of suspension method adapted appropriately to the tests on non-sporulated anaerobes. 72 hours' cultures of strains containing 10(9) of live bacterial cells in 1 ml have been used as inoculum. MBC readings have been performed after 7 days' incubation at temperature of (37 degrees C) 310 degrees K. From the total number of 97 strains under examinations 34 strains were sensible to low concentration of Sterinole equal to 7.8 to 15.5 micrograms/ml. Further 19 strains exacted MBC within 15.6 to 31.1 micrograms/ml and further 40 strains perished in the antiseptic concentration of 31.2 to 62.4 micrograms/ml. The remaining 4 strains exacted concentration of 62.5 to 125 micrograms/ml to be perished. Among 97 strains 29 strains were perishing in low concentrations of cetylpirydine chloride equal to 7.8 to 15.5 micrograms/ml. Successive 25 strains were damaging by the antiseptic concentration equal to 15.6 to 31.1 micrograms/ml. Further 28 strains exacted utilization of concentration equal to 31.2 to 62.4 micrograms/ml while in case of the remaining 15 strains MBC values achieved the level of 62.5 to 125 micrograms/ml. Strains from Leptotrichia buccalis and then Gram--positive anaerobic bacteria were the most sensitive ones both to Sterinole and cetylpirydine chloride while Gram--negative anaerobic bacteria and anaerobic micrococcus were less sensitive.

Bacteria, Anaerobic↗

PubMed1988