Comparison of antigenemia (pp65) assay and polymerase chain reaction in diagnosis of cytomegalovirus infection in renal transplant recipients treated with ATG.
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Adult↗
Biomedical subjects
M Kańtoch
Publications and source records attributed to M Kańtoch.
Usefulness of hybridization and PCR methods in monitoring of CMV infection in renal transplant recipients.
The purpose of this work was to compare hybridization and PCR methods as diagnostic tests in diagnosing and monitoring CMV infection. The investigation was performed in a group of 24 renal transplant recipients treated with ATG. The results we obtained suggest that quantitative variant of hybridization is more useful in diagnosing the infection than PCR, because it enables to monitor the infection. DNA CMV level of about 60 pg/ml or the increasing level in the subsequent samples should be a sign to start antiviral therapy.
Adult↗
Identification of cytomegalovirus (CMV) infection by different laboratory methods in renal transplant recipients undergoing triple-drug immunosupressive treatment.
Early diagnosis of CMV infection is very important mainly in transplant recipients because CMV infection is a frequent complication after transplantation. In this work we compared different laboratory methods: ELISA (IgG, IgM), Western blot,shell vial, antigenemia assay (pp65), the immunofluorescent method with epithelial cells from urine (IF), DNA in leukocytes by PCR and DNA in leukocytes by hybridization (HCS) to estimate the most proper method for diagnosis of CMV in renal transplant recipients. This preliminary study showed that HCS, PCR and Western blot are sensitive methods for detecting CMV infection. Using HCS in quantitative variant we obtained a very good correlation between DNA load and clinical symptoms.
Adult↗
[Papillomaviruses and herpesviruses as selected risk factors in the etiopathogenesis of cervix neck cancer. I. Use of nucleic acid hybridization for diagnosing HPV infections].
HPV infections are regarded as the main etiological factor responsible for the presence of cytological abnormalities and the primary risk factor for cervical cancer development. Diagnostic materials were collected between 1995 and 1997 in the Gynecologic Cancer Prevention, Cancer Center Institute of Oncology, Memorial Hospital Maria Skłodowska-Curie, Warsaw. The patients were divided into three groups: group C--women suspected for viral infection during clinical or cytological examination and two comparative groups A and B--women invited for routine cytological examination. Cytological smears for nucleic acid hybridisation collected before cytological smear sampling with a dacron swab (groups A and C) or collected after cytological smear sampling with a cervical brush (group B) were used. Cytological and clinical data was also used in the investigation. In 52% of the 236 samples tested by nucleic acid hybridisation HPV DNA was found to be present. DNA from the high/intermediate HPV risk group was also present in 36% of the samples and in 11% of the samples from the low risk HPV group. In 5% of the samples we confirmed the presence of mixed infections from both HPV risk groups. The results obtained from nucleic acid hybridisation with pap smear results were compared. It was observed that HPV infections from the high risk group occurred more frequently in pap 3 and pap 4 test results; HPV infections from the low risk group occurred more frequently in pap 1 and pap 2 results, while mixed infections from both risk groups occurred particularly in pap 4 and pap 5 results. Women in the 35-45 age groups suffered more frequently from infections from the high/intermediate risk group. In the 25-35 and 55-65 age groups HPV infections from the low risk group occurred more frequently. In the comparative groups only 2.6% of the women were infected with HPV.
Adult↗
[Papillomaviruses and herpesviruses as selected risk factors in the etiopathogenesis of cervix neck cancer. II. Use of PCR for isolating DNA of human papillomavirus and Herpes simplex].
The aims of the study were to compare polymerase chain reaction PCR with nucleic acid hybridisation HC in the routine diagnosis of HPV infections. Smears collected for PCR were digested for 24 hours using proteinase K. After DNA extraction 174 samples were tested by PCR with human bglobin primers PG04-GH20. The PCR products were separated in 2% agarose gel electrophoresis stained with ethidium bromide. In 80.6% of the samples 256 base pair DNA fragments were observed in the gel in UV light. These samples were tested by PCR with HPV primers MY09-MY11. In 40% of the samples the presence of HPV DNA was confirmed. Next we carried out PCR using a mixture of two pairs of primers bglobin PG04-GH20 and HPV MY09-MY11. DNA for this study was extracted from 24 samples in which the presence of human DNA was not confirmed in the first PCR test and from 7 untested samples. In 21 cases HPV DNA was found to be present in gel electrophoresis. The presence of HPV DNA was confirmed in 44.75% of the samples.
Adult↗
["Shell vial" method in diagnosis of cytomegalovirus infections].
The paper presents description of "shell vial" method and results obtained in the study of 331 materials derived from 3 different groups of patients. Problems of referring materials to laboratory, choice of type of material and method of staining are discussed. Also advantages (detection of virus capable of replication) and disadvantages (low sensitivity, toxicity of materials for detector cells) are discussed.
Blood↗
[Tick-borne encephalitis--etiopathogenesis and implications for public health in Poland].
Tick-borne encephalitis (tbe) belongs to infectious units being under study in Poland since over 40 years: clinical, virological, immunological, epidemiological (see eg. fig. 1 of the review) observations succeeded in developing tbe map of Poland, and organization of satisfactory diagnostic virological control of the disease. This article covers most important data since the early (1952-1953) expeditions to the endemic districts of Poland, studies of human beings, animal reservoir, both wild and domestic animals, biological vectors, migrating and local birds, frequency of virus isolations from man, ticks, mosquitos, wild rodents, with special reference to specific (vaccines, immunoglobulins) prophylaxis and associated unexpected and negative reactions. The article points also on north-east districts as of high tbe exposure, on described milk-associated outbreaks. The problems are discussed in relation with analogical and/or probable reactions related with other viral infections. Because of so meritorically differentiated and long-time performed studies and observations, the tbe is still in center of medical and public interest in Poland.
Adult↗
Serological confirmation of human herpesvirus type 6 infection in different age groups.
Human herpesvirus type 6 (HHV-6) infection was proved by IgG immunofluorescence method. 354 sera at various age groups were tested and 161 sera from children in more detail. There was no significant differences in our results and obtained by other authors, when testing sera from adults. Our investigation of younger children sera confirmed that mother antibodies are transferred, but also suggest the possibility of virus vertical infection. Our study also confirmed that antibodies are acquired very early in life in the same age group as exanthema subitum does.
Adolescent↗
Comparative analysis of HSV-1 temperature mutants proteins and their reactivity.
Protein's characteristic of temperature sensitive (ts 28 degrees C) and temperature resistant (tr 39 degrees C) mutants and native HSV-1 strain was performed. Electrophoretic and Western-blott analysis of virus proteins and their reactivity tested by immunoenzymatic staining was the main subject of the presented paper. There were found no significant differences in the protein structure between native strain and its temperature clones in electrophoresis. It is important that slight differences observed in protein's antigenic reactivity of ts 28 degrees C (54 kDa) and tr 39 degrees C (99-100; 160 kDa), do not influence on the possible application of the mutants for immunization and challenging.
Antigens, Viral↗
Viral vaccines: selected topics.
Significant role of viruses in pathology, their dominating position in etiology of infectious diseases point at the special position of active prophylactic procedures based on vaccination. The real role and value of viral vaccines of classic and modern generations, the limitation of immune potency in suppression of defence mechanisms, some problems of immunization against virus vertical transmission are presented in the paper. The reader may find tables which cumulate selected but significant patterns of viral vaccines and vaccinations, and selected papers devoted to topics discussed.
Adult↗
Studies on the stability of HSV-1 temperature sensitive 28 degrees C clone.
The temperature mutants of herpes simplex virus type 1 has been received. The following properties on different levels of passages were investigated: stability of virulence, virus infection titer and immunogenicity. The virulence stability was expressed by percent of survivors and mean survival time in infected mice (CFW/Pzh and BALB/cPzh strains). Virus infection titer's stability was measured after prolonged exposure of temperature sensitive mutant (ts 28 degrees C) to critical temperatures (+4 degrees C and from +20 degrees C to +23 degrees C). Stability of immunogenic potency of ts 28 degrees C was evaluated in mice immunized with the virus on different level of passages and after that they were challenged with highly pathogenic temperature resistant clone (tr 39 degrees C). The performed experiments proved the stability of investigated features.
Animals↗
[Possibilities and etiologic problems in prophylaxis of human virus infections].
There are areas of proved and successful prophylaxis of human's virus infections: vaccines, gammaglobulins and synthetic viral inactivators. In case of vaccine application the very important tasks concern situations of decreased immunological potency and viral latency. The progress and up to now achievements according to viral vaccines which may be used in immunocompromised people are presented in the paper, as well as the specificity of studies on vaccines against latent viral infections. Gammaglobulin preparations especially of commercial production, are characterized. Mechanism of their activity, different efficiency in viral infections and problems of safety, point on the important complementary role of gammaglobulin prophylactic application. Disinfecting agents grow in importance, so the main topic presented concerns the requirements, which must be fulfilled to achieve satisfactory results in preventing virus infections.
Adult↗
Studies on etiopathogenesis and immunity in viral infections.
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Animals↗
[Antiviral activity of IgG subclasses of immunoglobulin preparations for intravenous use].
Preparations of human immunoglobulins for intravenous use (IVIG) should contain proper percentage of IgG subclasses, responding to physiological preparations with preserved activity of antibodies. The study was aimed at establishment of activity against measles, CMV and HSV viruses in individual subclasses of Bioglobulin (Biomed, Warszawa) and a comparison with preparation Polygam (Baxter, USA). Indirect immunofluorescence test was used. The results revealed presence of antiviral activity mostly in subclass IgG1 and also in IgG3, which is in keeping with results obtained by other authors. We have found an anti-HSV activity in a subclass IgG2 and IgG4 in IVIG preparations which was not yet described in the literature; it is confirmed, however, in investigations with application of sera of HSV-positive persons. These discrepancies may be caused by different methods of detection. Viral antigens in ELISA and IF tests may differ among themselves by content of individual epitopes. Basing on obtained results it can be stated that Polish preparation Bioglobulin (in which for the first time antiviral activity was determined in subclasses) is comparable with Polygam.
Antibodies, Viral↗
[Seroconversion after vaccine with trivalent influenza vaccine during the epidemic season 1990/1 in Poland].
Vaccination was performed by influenza virus vaccine, trivalent type A and B. We vaccinated about 50,000 persons in Poland in different groups of age. HI and NI tests were used for testing antibody level for A/Taiwan/1/86 (H1N1), A/Shanghai/16/89 (H3N2) and B/Yamagata/16/89. The rise of antibody level was statistically significant.
Adolescent↗
[Influence of high dose intravenous immunoglobulins (IVIG) on HSV-1 infection in immunosuppressed mice].
Influence of low and high doses of intravenous immunoglobulin (IVIG) was compared on the basis of survival studies of mice infected with temperature sensitive (ts 28 degrees C) clone of Herpes simplex virus (HSV-1) after previous treatment with cyclophosphamide (CY), Sandoglobulin containing intact IgG and Gamma-Venin containing only fragments of (Fab)2 IgG were used. It was found that 4 and 16 mg doses protected animals in contrast to the 32 mg dose of Sandoglobulin which protected mice in only small degree.
Animals↗
[Use of western blotting methods in diagnosis of viral infection causing tick-borne encephalitis].
A Western blot method for investigation of antigens and of immunological response to tick-borne encephalitis was prepared. An analysis was performed testing cross reactions of proteins of prepared antigen with sera for four Polish strains of tick-borne encephalitis and four other flaviviruses. Usefulness for diagnosis of infections in humans was determined by comparison of Western blot method with ELISA immunoenzymatic test. It was found that elaborated method permits equally as ELISA for evaluation of immune response in immunoglobulin classes. By evaluation of response for individual virus components, Western blot method enables at the same time verifications of nonspecific determinations which result from reaction of antibodies present in tested diagnostic materials with nonviral antigens.
Antibodies, Viral↗
[Laboratory problems of chemotherapy of viral infections in humans].
The following problems are presented and discussed: scientific and epidemiologic basis of chemotherapy, relations of antiviral activity in vitro and in vivo especially important when new-drugs are developed and evaluated, inhibitors used in medical treatment and mechanisms of their antiviral functions, genesis and nature of virus resistance, possible role of interfering defective particles. The paper is addressed both to laboratories involved in new drugs construction as well to clinical ones performing etiologic diagnostic procedures.
Acquired Immunodeficiency Syndrome↗