Biomedical subjects

M Kaczmarski

Publications and source records attributed to M Kaczmarski.

At least 19 recordsLinked to original sources

PubMed2005

Phase diagram of silicon from atomistic simulations.

In this Letter we present a calculation of the temperature-pressure phase diagram of Si in a range of pressures covering from -5 to 20 GPa and temperatures up to the melting point. The phase boundaries and triple points between the diamond, liquid, beta-Sn, and Si34 clathrate phases are reported. We have employed efficient simulation techniques to calculate free energies and to numerically integrate the Clausius-Clapeyron equation, combined with a tight-binding model capable of an accuracy comparable to that of first-principles methods. The resulting phase diagram agrees well with the available experimental data.

Journal Article↗

PubMed2005

Correlations between leptin, body composition, bone mineral density, and bone metabolism in kidney transplant recipients.

INTRODUCTION: In kidney transplant recipients leptin levels are often elevated and bone mineral density (BMD) decreased. However, to date there are no about correlations between leptin and BMD in this population. It has been suggested that leptin is a predictor of BMD in postmenopausal women. Moreover, leptin acts as a marker of fat stores. We examined the relationships between leptinemia, some markers of nutritional status, BMD, and bone metabolism in kidney transplant recipients. We also assessed whether leptin was a significant and independent predictor of BMD in this population. METHODS: BMD and fat content (global, percentage, trunk) were measured using dual-energy X-ray absorptiometry in 27 kidney allograft recipients. Markers of bone turnover and leptin were studied using commercially available kits. RESULTS: Leptin correlated with the percentage of body fat, trunk fat, lean body mass, serum creatinine, and urea. Insulin growth factor binding protein 1 was negatively related to waist-hip ratio and global and trunk fat, whereas BMD of the lumbar spine was correlated with the daily dose of prednisone, azathioprine, cyclosporine trough levels, serum calcium, as well as osteoprotegerin level. CONCLUSIONS: Leptin levels are associated with graft function and body fat in kidney allograft recipients. Leptin is not related to nutritional status, BMD, or bone metabolism in kidney allograft recipients, but is associated with the current dosage of immunosuppressants and the serum calcium.

Absorptiometry, Photon↗

PubMed2005

The role of the therapeutic team in shaping eating habits and lifestyle in children with dietary calcium deficiency.

PURPOSE: Assessment of the effect of low-calcium diet on bone mineral content in children and adolescents. MATERIAL AND METHODS: The study involved 89 children (49 girls and 40 boys) aged 5-18 years, in whom diseases affecting bony metabolism had been excluded. Children with a history of dietary calcium content below 500 mg/day were recruited. The study group was divided according to age: group I, age 5-9 years (children before puberty); group II, age 9-15 years (early puberty); group III, 15-18 years (late puberty). Dual energy X-ray absorptiometry (DEXA) was used for densitometric measurements. Bone mineral density (BMD) was assessed in the whole skeleton (total BMD), in vertebrae L2-L4 (spine BMD) in g/cm2 and as Z-score. Concentrations of Ca, Ca2, P, activity of alkaline phosphatase (AP) and its bony isoenzyme were determined in the serum. RESULTS: Total bone mass below 5th percentile (according to the norm for age and gender) was found in 56.98% of the children involved in the study. A significant reduction was noted in the spine mineral mass in boys (p < 0.01) as compared to girls (0.731 +/- 0.17 g/cm2 and 0.835 +/- 0.19 g/cm2, respectively). The lowest mean Z-score (-1.850) was observed in group III as compared to group I (-1.194) (p < 0.01) and group II (-1.201) (p < 0.05). There were statistically significantly positive correlations between total and spine BMD and BMI. The correlation coefficient was r = 0.56 and r = 0.41 (p < 0.001), respectively. CONCLUSIONS: In the majority of the children (c. 60%), a reduction in bone mineral content was found. The lowest Z-score (-1.850) was revealed in the oldest children, which may disturb the process of reaching the optimum level of the peak bone mass.

Adolescent↗

PubMed2005

Helicobacter pylori eradication as prevention against chronic peptic ulcer disease in children.

The changes caused by Helicobacter pylori are a slow, progressing inflammatory process developing from several to dozen years. H. pylori infection leads to an inflammatory response in the gastric mucosa with granulocyte infiltrates in an acute form of the inflammation, and lymphocytes, plasmatic, macrophages and eosinophils in a chronic form inducing the development of gastric and duodenal ulcers and gastric cancer in some patients. The frequency and the type of morphological changes in the gastric mucosa were analyzed in children with positive IgG against H. pylori and the incidence of gastric and duodenal ulcers in family members of children examined was evaluated in our study. Gastritis was reported in 68.8% of children with positive IgG against H. pylori. Gastric ulcer was confirmed in 37.1% of families of children included in the study. Duodenal ulcers were found in 22.9% of families. The results obtained, indicate the usefulness of long-term observation and clinical follow-up of children with chronic gastritis of H. pylori ethiology taking into consideration bacterium eradication as prophylaxis of peptic ulceration.

Adolescent↗

PubMed2005

Cholecystokinin octapeptide (CCK-8) concentration in plasma is not affected in functional abdominal pain in children.

PURPOSE: Cholecystokinin regulates gut motility and visceral sensation. The aim of the study was to determine the diagnostic value of plasma cholecystokinin octapeptide (CCK-8) concentration in children with functional abdominal pain (FAP). MATERIAL AND METHODS: Fifty-two children (33 girls and 19 boys) aged 6-17 years with chronic abdominal pain were included in this study. On the basis of clinical data, results of endoscopy and Criteria for Functional Disorders the patients were divided into three groups: group 1--functional dyspepsia (FD), group 2--irritable bowel syndrome (IBS), group 3--non-specific FAP. The control group consisted of children without abdominal pain in anamnesis. CCK-8 concentrations in plasma were measured with radio immunoassay technique, after plasma extraction. In study protocol we analysed CCK-8 levels in fasting state and 15, 30, 60 minutes after a standard test meal. RESULTS: In the fasting state plasma levels of CCK-8 were similar in each group and in controls. In the IBS patients CCK-8 levels were not increased after meal. In groups 1, 3 and controls postprandial levels were higher when compared to fasting state (p<0.05). Area under curve of CCK-8 plasma concentration was the lowest in group 2, but not significant compared to controls and other groups. No correlation was found between main symptoms of FD and IBS and CCK-8 concentration in plasma. CONCLUSIONS: We conclude that gut dysmotility and symptoms of functional abdominal pain in children are not concerned with alteration of plasma CCK-8 levels before and after meal.

Abdominal Pain↗

PubMed2005

Atopy patch test in the diagnosis of food allergy in children with atopic eczema dermatitis syndrome.

PURPOSE: Food allergy has been demonstrated to play an important role in the pathogenesis of atopic eczema dermatitis syndrome (AEDS), affecting often atopic infants and young children. The most commonly offending foods are cow's milk, hen's egg, wheat and soy; implicating immediate (IgE-mediated) and late-phase (T-cells) immunological reactions in the pathogenesis of skin lesions. The diagnostic work-up of suspected immediate food reactions includes skin prick tests (SPT) and the measurement of food-specific antibodies (sIgE). The methodology of atopy patch test (APT) has been reported as a diagnostic tool with high predictive capacity for late-phase clinical reactions in children with atopic dermatitis. Although APT has been introduced into the diagnostic procedure for food allergy, its diagnostic accuracy remains still controversial; especially in older children. The aim of study was to evaluate the diagnostic accuracy of the atopy patch test in the detection of food allergy in correlation with SPT, sIgE and positive oral food challenge to milk, in children suffering from AEDS and to assess the sensitivity and specificity of this method in dependence on the age of investigated children. MATERIAL AND METHODS: 34 children (25 boys, 9 girls) aged 5 months-16 years with suspicion of milk-related AEDS were investigated. These patients were subdivided into 2 age groups: group A--20 children (<3 years), group B--14 children (>3 years). The diagnostic procedures as skin-prick tests and atopy patch test were performed. The specific IgE to cow's milk allergens were also measured. The open and blind diagnostic oral food challenge were performed to verify the results of tests. Sensitivity, specificity, positive (PPV) and negative (NPV) predictive value of APT were calculated in both age groups. RESULTS: A positive challenge response to milk was found in 65.0% of investigated children in group A and in 35.7% in group B. No statistical differences in the prevalence of immediate (p<0.1905) and delayed-type (p<0.409) reactions has been found between age groups. Positive APT to milk were noticed in 55.0% of patients in group A and in 35.7% of children from group B, that has been in correlation with positive delayed-type reactions in oral food challenge in 72.7% and 80.0% in corresponding age groups. Polysensitization to other food allergens confirmed by SPT and/or sIgE was detected in 35.0% of patients younger than 3 years of age and in 50.0% of older children. The prevalence of positive APT to other foods (soy, rice, maize, cereals) was significantly higher (p<0.0073) in the polysensitized children from group A. Sensitivity of SPT/sIgE in children with immediate-type reactions to milk was 100%, specificity 94%. Sensitivity of APT to cow's milk in children with late-phase reactions was 80% in both age groups; specificity 70%/89% with comparable PPV in both groups (73%/80%). Parallel skin testing with combined patch test and evaluation of sIgE enhanced the value of sensitivity to 92% in the group A and specificity to 89% in the group B. For PPV corresponding figures were 85%/80%. CONCLUSIONS: APT was found to be more sensitive and specific method than SPT/sIgE in diagnosing delayed food allergy in children with AEDS. No age correlation between positive results of APT and oral food challenge and higher specificity of APT in older children confirm its accuracy in diagnosing delayed cow's milk allergy in all age groups of children. Combined skin prick and patch testing significantly enhances identification of food allergy in children with AEDS. The outcome of the APT with food does not seem to be influenced by age of children, but because of its variability of sensitivity and specificity, a diagnosis of food allergy should be confirmed by oral food challenge.

Adolescent↗

PubMed2005

Immunoblotting in the diagnosis of cross-reactivity in children allergic to birch.

PURPOSE: The scientific experiments with new immunological methods (immunoblotting, RAST inhibition) and isolation of recombinant allergens suggest structural similarities in the allergenic components responsible for cross-reactions. Immunochemical and molecular biology studies indicate that epitopes of major allergen (Bet v 1, Mal d 1) contain more IgE binding epitopes than minor allergens (Bet v 2, Mal d 2), what explained clinical importance of major birch and apple allergens, but it is individual. The important role in cross-reactivity play also proteins with low molecular weight; a potentially dangerous allergen is lipid transfer protein (LTP) inducing severe systemic reactions in allergic subjects. The recent studies indicate that the IgE cross-reactivity patterns and the clinical relevance is still not clear and that only some of patients with confirmed IgE cross allergy to Bet v 1 and Mal d 1 demonstrated clinical symptoms after ingesting of apple. The aim of study was to establish the pattern of cross-reactivity between major (Bet v 1) and minor (Bet v 2) birch pollen allergens and apple proteins in children allergic to birch using recombinant allergens and immunoblotting method. MATERIAL AND METHODS: The prospective study were carried out on the group of 13 children aged 4-16 years, referred to the IIIrd Department of Paediatrics in Białystok and outpatient clinic with clinical symptoms of food and inhalant allergy. Inclusion criteria to the study were: allergy to birch pollen recombinant allergens and apple, confirmed by presence of specific IgE in the sera of patients. The allergens from peel and pulp of apple and birch were separated and loaded onto the polyacrylamide electrophoretic gel and than transferred to membranes by Western blotting. Antigen-IgE complex was detected using goat anti-human IgE antibodies labelled with alkaline phosphatase. RESULTS: Only few sera presented strong reactions in immunoblotting to birch pollen proteins with a molecular weight of 17-18 kDa, corresponding to the main birch allergen Bet v 1. Most of sera having positive reaction vs Bet v 1 showed cross-reactivity with Mal d 1. All sera recognized specifically the main allergen of apple peel Mal d 3 with molecular weight < 10 kDa (Lipid Transfer Protein). CONCLUSIONS: Immunoblotting method allows to verification of cross-reactivity recognized by presence of specific IgE. The nature of proteins responsible for sensitization can influence the spectrum of offending foods and the clinical features of allergic reactions.

Allergens↗

PubMed2005

Hypersensitivity to hydrolyzed cow's milk protein formula in infants and young children with atopic eczema/dermatitis syndrome with cow's milk protein allergy.

PURPOSE: Atopic eczema/dermatitis syndrome (AEDS) is often the first manifestation of atopic disease in children. Food hypersensitivity should be considered in approximately 40% of these patients. AEDS children with cow's milk allergy are commonly prescribed a hydrolyzed formulas or amino acid-based formulas for an alternative protein source. The aim of this study was to investigate hypersensitivity to extensive hydrolyzed casein and whey proteins in AEDS children with cow's milk protein allergy (CMA). MATERIAL AND METHODS: The study included 67 hospitalized children with AEDS (m/f--43/24), aged 1-28 months (mean 11.34 +/- 8.52) and CMA confirmed by oral food challenge. All patients were treated with extensively hydrolyzed formulas: 48/67 children with casein hydrolysates and 19/67 children with whey hydrolysates. RESULTS: In most of studied children we recognized severe AEDS (SCORAD Index: mean 55.41 +/- 17.4; 95% CI 51.17-59.66) with elevated total IgE (mean 432.98 +/- 1030.46; 95% CI 181.63-684.33). In 22/67 children (32.8%) we established diagnosis of hypersensitivities to hydrolyzed formula (HHF): in 17/22 to casein hydrolysates, in 4/22 to whey hydrolysates and in 1/22 to amino-acid based formula. Children with HHF did not differ in the severity of AEDS evaluated by SCORAD (57.18 +/- 16.59 vs 54.56 +/- 17.90), the serum level of total IgE (603.9 +/- 1253 vs 349.4 +/- 906.1) and the time of breast-feeding (4.4 +/- 4.0 months vs 6.8 +/- 7.28). They differ in the number of plasma eosinophils and positive correlation between number of eosinophils and serum level of total IgE (p<0.05, r=0.46 vs r=0.07). CONCLUSIONS: Children with moderate or severe atopic eczema/dermatitis syndrome can demonstrate hypersensitivity to hydrolyzed formula recommended for therapeutic indications.

Allergens↗

PubMed2004

Sleep-related breathing disorders in small children with nocturnal acid gastro-oesophageal reflux.

PURPOSE: Coincidence of gastroesophageal reflux disease with obstructive sleep apnea/hypopnea syndrome has been discussed in recent years. Treatment with nasal continuous positive airway pressure (nCPAP) reduces gastroesophageal reflux (GER) in adult patients with obstructive sleep apnea (OSA). Moreover, treatment of gastroesophageal reflux with omeprazole can reduce the severity of obstructive sleep in selected individuals. The aim of the study was to test the hypothesis that gastroesophageal reflux does not influence sleep quality and breathing pattern during sleep in children. MATERIAL AND METHODS: 24 children (14 boys, 10 girls, aged 2 months-3 years) with sleep disturbances indicating GER were studied. Standard polysomnography with parallel recording of 24-h oesophageal monitoring was performed. Apnea/hypopnea index (AHI) in active/REM sleep and quiet/NREM sleep was compared between nocturnal acid GER children (13 children; 7 boys, 6 girls; aged 1.28 +/- 0.95y; FRT-18.63 +/- 11.83%) and nocturnal acid GER-free controls (11 children; 7 boys, 4 girls; aged 1.64 +/- 0.97y; FRT-2.93 +/- 2.08%). Exclusion criteria were: 1. laboratory signs of infection (increasing OB, increasing CRP, increasing alpha2-globulin); 2. clinical symptoms of infection in the respiratory tract, the alimentary tract or in the urinary tract. RESULTS: In children with nocturnal GER higher incidence of obstructive apnea/hypopnea during REM sleep was found: AHI = 23.35/h +/- 19.1; (CI 95%11.81-34.89) vs AHI = 4.99/h +/- 3.12 in children without nocturnal GER. We found no differences between the groups in saturation < 90% time during sleep. CONCLUSIONS: The study confirms coincidence of nocturnal gastroesophageal reflux and sleep-related breathing disorders in children. Higher number of apnea/hypopnea during REM sleep was found in children with nocturnal gastroesophageal reflux.

Case-Control Studies↗

PubMed2004

Diagnostic value of birch recombinant allergens (rBet v 1, profilin rBet v 2) in children with pollen-related food allergy.

PURPOSE: Pollen-related food allergy to fresh fruits and vegetables is a well-known clinical phenomenon. Bet v 1, the major birch pollen allergen, has been cloned and shows homologies to various food allergens (e.g. hazelnut, apple, celery, tomato). Allergy to profilin Bet v 2 was also described in 10-15% of patients sensitized to birch pollen. Objective of our work was to assess the diagnostic value of recombinant allergens (rBet v 1, rBet v 2) for diagnosis of children sensitized to birch pollen with associated food allergy. MATERIAL AND METHODS: The investigations were carried out on the group of 14 children aged 4-17 years, with a history of allergic reactions and sensitized to birch pollen with associated food allergy. Skin prick tests were performed with natural foods and commercial aeroallergens (Bencard). Sera-specific IgE antibodies to recombinant and other allergens (Pharmacia Upjohn) were measured with a fluoroimmunoenzymatic essay (UniCAP). Oral food challenge tests were performed to confirm adverse food reactions. RESULTS: 64% were sensitized to rBet v 1, 14% to rBet v 2, 7% to both of them. 50% of children with allergy to Bet v 1 had also concomitant allergy to other pollens and food allergy to fruits from family Rosaceae. Patients with positive reaction to Bet v 2 represented allergy to vegetables from family Umbelliferae. The most common form of allergic reactions were: allergic rhinitis in 64%, atopic dermatitis in 36%, oral allergy syndrome in 21% of investigated children. CONCLUSIONS: Use of two recombinant allergens permits the diagnosis of birch pollen sensitization in children with food-pollen related allergy and gives the pattern of possible cross-reactivity between pollen and food allergens in children with allergic diseases.

Adolescent↗

PubMed2004

Cytotoxic lymphocytes (CD8+) in the antrum mucosa in children with chronic Helicobacter pylori-related inflammation before and after bacteria eradication.

The authors assessed the expression of cytotoxic CD8 lymphocytes in the antrum mucosa of children with chronic Helicobacter pylori-related inflammation, before and after bacteria eradication. Biopsy specimens of gastric mucosa were evaluated in specimens, collected from 59 H. pylori-positive patients (Group I), 29 patients after H. pylori infection (Group II) and 18 H. pylori-negative children (Group III). The obtained specimens were assessed for infection and inflammation and the expression of CD8+ lymphocytes was estimated, using monoclonal antibodies. The number of CD8+ lymphocytes in the mucosa was counted. The results of the study showed an increase in the expression of CD8+ lymphocytes in children with H. pylori infection, in comparison to the values in children after bacteria eradication. The increased expression of CD8+ lymphocytes correlated with the severity degree of antrum gastritis.

Biopsy↗

PubMed2004

Helicobacter pylori infection and mast cells of the antrum mucosa.

The studies aimed at evaluating mast cells in inflammatory infiltration of gastric mucosa in children with H. pylori infection, as well as in those after the infection eradication. Biopsy specimens of gastric mucosa were evaluated, the specimens collected from 59 H. pylori-positive patients (Group I), 29 patients after H. pylori infection (Group II) and 18 H. pylori-negative children (Group III). The specimens were assessed for infection and inflammation and stained with anti-human mast cell tryptase to count mucosal mast cells. The evaluations of histopathological changes in the antrum mucosa of the children were performed, according to Sydney's Classification. In morphometric evaluation, slight differences were found in the numbers of mast cells among Groups: I, II and III of the examined children (the number of mastocytes being: 86.4, 81.4 and 70.2 cells/mm2 of specimen, respectively).

Adolescent↗

PubMed2004

B (CD20+) lymphocytes in the antrum mucosa of children with Helicobacter pylori infection.

The aim of the study was to estimate the expression of CD20+ lymphocytes in the antrum mucosa in children, infected with Helicobacter pylori and after bacteria eradication. Biopsy specimens of gastric mucosa were the specimens, collected from 59 H. pylori-positive patients (Group I), 29 patients after H. pylori infection (Group II) and 18 H. pylori-negative children (Group III). The collected specimens were assessed for infection and inflammation and the expression of CD20+ lymphocytes was estimated, using mice monoclonal antibodies. The expression of CD20+ lymphocytes in the inflammatory infiltrate of the antrum mucosa correlated with the severity of gastritis, found in children with Helicobacter pylori infection and was the highest in comparison with the group of children after H. pylori eradication.

Antigens, CD↗

PubMed2004

Memory cells in the antral mucosa of children with Helicobacter pylori infection.

A total of 106 patients, included in the study, were divided into three groups with regard to Helicobacter pylori infection. Endoscopy and histopathological examination of the stomach, based on the Sydney's System, were performed in all the children. CD45RA and CD45RO cells were identified by means of specific antibodies in the inflammatory infiltrate of the antral mucosa. An increased expression of CD45RO and CD45RA lymphocytes was reported, basing on the results of the study.

Adolescent↗

PubMed2004

Macrophages of the antral mucosa in children with Helicobacter pylori infection and after eradication.

Our study included 59 children, aged 12.2 +/- 4.6 years, with Helicobacter pylori infection and 29 children, aged 11.0 +/- 4.2 years, with past H. pylori infection after spontaneous eradication with positive IgG antibodies against H. pylori and with functional disorders of the gastrointestinal tract, without H. pylori infection, with normal IgG concentration against H. pylori. All biopsy specimens from each of the study groups were stained by an immunohistochemical method for the evaluation of CD68+ macrophages in the antral mucosa. Histopathological changes in the antral mucosa of children with Helicobacter pylori are characterized by an increased infiltrate of macrophages, dependent on the severity grade of inflammation.

Adolescent↗

PubMed2003

Correlations of new markers of bone formation and resorption in kidney transplant recipients.

Renal osteodystrophy is a common complication of chronic renal failure and renal replacement therapy. Successful kidney transplantation reverses many of these abnormalities, but the improvement is often incomplete. The evaluation of renal osteodystrophy in everyday practice is based on noninvasive measurements. Taking this into consideration the aim of the present study was to assess new markers of bone metabolism: serum CrossLaps degradation products of C-terminal telopeptides of type I collagen tartrate-resistant acid phosphatase (TRAP) and bone-specific alkaline phosphatase (bALP), as well as their correlations with bone mineral disease (BMD) in kidney transplant recipients. Twenty-six patients (aged 26 to 54 years) receiving a triple immunosuppressive regimen with stable graft function were enrolled in the study. Serum parathormone (PTH) osteocalcin type collagen C-terminal peptides (ICTP), and procollagen type I carboxyterminal extension peptide (PICP) concentrations were measured by radioimmunoassay (RIA), Serum CrossLaps, bALP, beta2-microglobulin, TRAP 5b by enzyme-linked immunoassay (ELISA), and deoxypyridinoline (DPD) in urine immunochemiluminescence. BMD, as measured by dual-energy X-ray absorptiometry (DEXA), correlated negatively with markers of bone formation (bALP, osteoclacin, and PICP) and resorption (TRAP, ICTP, and beta2-microglobulin). The only positive correlation was between urine DPD and BMD at the femoral neck. Interestingly, BMD correlated negatively with CsA concentration. TRAP 5b correlated positively with serum creatinine, ALP, bALP, osteocalcin, iPTH, ICTP, and serum beta2-microglobulin, and negatively with CsA concentration, and azathioprine and prednisone dose. DPD did not correlate with any parameters. Serum CrossLaps correlated with markers of both bone formation and resorption. Because TRAP and serum CrossLaps correlated with markers of both bone formation and or resorption, additional studies are needed to establish the value of these markers of bone resorption to assess renal osteodystrophy.

Adult↗

PubMed2003

Peripheral blood lymphocyte population in children infected with Helicobacter pylori.

PURPOSE: Helicobacter pylori infection in children is associated with a chronic inflammatory process of gastric and duodenal mucosa, which may have a various clinical course ranging from asymptomatic and chronic inflammatory condition to gastric ulceration. The immune system may contribute especially to chronic gastric mucosa inflammation. The aim of our study was to assess the levels of peripheral blood T (CD3+, CD4+, CD8+) and B lymphocyte subpopulation (CD19+) in children with Helicobacter pylori infection and to evaluate their relation to degree of antrum mucosa inflammation. MATERIAL AND METHODS: The study was performed in 32 children aged 7-18 years, hospitalized due to dyspeptic symptoms. The endoscopic examination of upper gastrointestinal tract was performed and gastric and duodenal mucosa was estimated in all patients. The endoscopic and histological evaluation of gastric mucosa was performed according to the Sydney System [4]. The urease test (CLO-test-H. pylori) was made to estimate the severity of the infection. RESULTS: Moderate antrum mucosa inflammation was found in 41.2% of the examined. The highest percentage of children (58.8%) presented marked inflammation. No mild inflammation was found in children examined. CONCLUSIONS: No correlation was found between lymphocyte levels and the degree of the inflammatory changes in antrum mucosa. The evaluation of peripheral blood lymphocytes performed in children with Helicobacter pylori infection suggests that T lymphocytes may play a predominant role in this infection.

Adolescent↗

PubMed2003

Comparative evaluation of gastric mucosa morphological changes in children and adults with positive IgG antibodies to Helicobacter pylori.

PURPOSE: Helicobacter pylori colonization of gastric epithelium causes a local and systemic, cellular and humoral immune response. Despite this immune response involvement in the infection, its elimination from the organism does not take place and the process usually becomes chronic. The purpose of the study was to establish the prevalence of gastric mucosa inflammation in children and adults with serum positive anti-Helicobacter pylori antibodies IgG. MATERIAL AND METHODS: The study included 171 patients comprising 109 (63.7%) children and 62 (36.3%) adults with IgG positive titre against Helicobacter pylori, who were qualified to the study basing on epidemiological examinations estimating the prevalence of Helicobacter pylori infection in the population of north-eastern Poland living in the country, town and city. All patients reported dyspeptic symptoms. The evaluation was performed basing on the morphological (endoscopy) and histopathological examinations estimating the changes in gastric mucosa of these patients. RESULTS: The evaluation of antrum and corpus gastric mucosa proved normal gastric mucosa in 34 children (31.1%) and 10 adults (16.1%) with positive IgG antibodies against Helicobacter pylori. The evaluation of the severity showed the predominance of moderate inflammation within corpus in children (37.6%) and marked inflammation in adults (45.1%). CONCLUSIONS: The concentration of IgG antibodies against Helicobacter pylori in both groups was highest in patients with marked antrum gastric mucosa inflammation.

Adult↗