[Toxic damage to liver cells and the effect of heavy metal salts on mixed function oxidase enzyme system activity].
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Alanine Transaminase↗
Biomedical subjects
M Kadiiska
Publications and source records attributed to M Kadiiska.
Effect of cadmium in vitro on benzphetamine-N-demethylation and ethoxyresorufin-O-deethylation in rats of different age.
The in vitro influence of CdSO4 on the liver microsomal benzphetamine-N-demethylation and ethoxyresorufin-O-deethylation activities in male Wistar rats/age 10, 30, 60 and 240 days/as well as the type of inhibition were studied after phenobarbital or beta-naphthoflavone pretreatment. Both reactions were inhibited by CdSO4 in dependence on concentration in all age groups. After phenobarbital pretreatment the inhibition of N-demethylation was slightly pronounced in 10-day old rats. In 10 and 30-days old rats the type of inhibition was competitive while in 60 and 240-days old rats a non-competitive type of inhibition was observed. O-deethylation of ethoxyresorufin was inhibited also in 10-day old rats, the inhibition type was non-competitive in all age groups. After beta-naphthoflavone pretreatment N-demethylation was inhibited competitively in all age groups. The inhibition of O-deethylation was more pronounced in 30-day old rats, in other age groups the inhibition was weak but concentration dependent. The type of inhibition was noncompetitive for all age groups studied.
Aging↗
Effect of acute intoxication with some heavy metals on drug metabolism.
In experiments on male albino rats after subcutaneous injection of a single toxic dose it was found that copper sulphate (CuSO4), cobaltous nitrate (Co(NO3)2, cadmium sulphate (CdSO4), lead acetate Pb(CH3COO)2 significantly inhibited the hexobarbital-oxidazing enzyme system, while with Nickel sulphate (NiSO4), Zinc sulphate (ZnSO4), Arsenic trioxide (As2O3), Mercuric chloride (HgCl2), Bismuth [Bi(NO3)2] and stannous chloride (SnCl2) the differences were insignificant. Aniline hydroxilase was significantly inhibited by CuSO4, Co(NO3)2, CdSO4, Pb(CH3COO)2, NiSO4, and As2O3 and not inhibited at all by HgCl2. Bi(NO3)2, SnCl2, and ZnSO4. In determination of ethylmorphine demethylase activity CuSO4, Co(NO3)2, CdSO4, and NiSO4 significantly inhibited the enzyme activity, while with the other salts the differences were insignificant. The metals which exerted an inhibitory effect on the above-mentioned enzymes reduced also the microsomal cytochrome P-450 content.
Animals↗