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Biomedical subjects

M Kahle

Publications and source records attributed to M Kahle.

At least 19 recordsLinked to original sources
PubMed2006

Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates.

Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.

Amino Acid Sequence↗
PubMed2000

[Isolated hyoid bone fracture].

We present a rare case of isolated hyoid bone fracture caused by direct trauma. We will describe the mechanism of injury, how to find diagnosis and our treatment.

Adolescent↗
PubMed2000

Megaplex DNA typing can provide a strong indication of the authenticity of ancient DNA amplifications by clearly recognizing any possible type of modern contamination.

Recent experiments revealed the perfect applicability of megaplex typing by autosomal short tandem repeats (STRs) to degraded DNA. The advantages of megaplex approaches lie in reduced amounts of sample material that are necessary and in remarkable time saving. Furthermore, megaplex typing clearly recognizes possible contaminations and thus has a large potential for indicating authenticity in ancient DNA analysis. This is demonstrated by three examples in which various types of contaminations could clearly be identified as such and even traced back to their origin. This would have been impossible using control samples, due to the sporadic nature of these types of contaminations.

Artifacts↗
PubMed1993

[Abdominal actinomycosis].

Twelve years after adnexectomy, performed for actinomycosis of the left ovary, a 32-year-old woman developed abdominal and back pain. A solid tumour was palpated in the left lower abdomen. Colon contrast examination revealed a subtotal stenosis in the sigmoid colon, while sonography showed a complete stenosis of the left ureter with left hydronephrosis. Relaparotomy demonstrated a stone-hard tumour at the rectosigmoid junction, which involved the right ovary and ureter, as well as having infiltrated the retroperitoneum, predominantly on the left. After resection of the sigmoid colon, uterus and right ovary, as well as of the ureteric stenosis with reanastomosis, the further course was without complication. Histological examination confirmed actinomycosis of the left ovary, sigmoid colon and pelvic mesocolon. Therapy with amoxycillin, 500 mg three times daily, was started. As the patient had worn an intrauterine pessary for several years, primary infection of the uterus with spread into the abdominal cavity via tube and ovaries is likely to have been the course of events. The recurrence was probably caused by reactivation of residual actinomycetes in the retroperitoneum and pelvic mesocolon.

Abdomen↗
PubMed1992

[Complicated aneurysm of the splenic artery].

For 2 1/2 years after sustaining blunt trauma to the abdomen a 51-year-old woman had experienced episodes of pain, recently with throbbing, in the left upper quadrant. A smooth, circumscribed, painless tumour filling the left upper and mid-abdomen was palpable under the left costal margin. Ultrasound revealed a 12 x 11 cm space-occupying mass with cystic-fluid components, at first interpreted as a blood-containing pseudocyst. Computed tomography demonstrated a connection with the splenic artery. Digital subtraction angiography established the diagnosis of an aneurysm of the splenic artery arising immediately beyond its origin from the coeliac trunk. Splenectomy and resection of the aneurysm were performed. Total removal of the aneurysm was not undertaken because the dorsal aspect of the aneurysm wall was very thick and fused with the retroperitoneum. The postoperative course was unremarkable. The genesis of splenic aneurysm often remains unexplained. Its occurrence after blunt abdominal trauma is exceedingly rare.

Abdominal Injuries↗
PubMed1992

[The dynamic condylar screw (DCS) in proximal femoral fractures. Initial experiences].

Beginning January 1st 1987 19 fractures of the proximal femur were stabilized utilizing the "dynamic condylar screw" (DCS). During or after reduction of the fracture the DCS is accomplished by a supporting plate. In the cases we applied the DCS hitherto employment of a bone distractor of complementary cancellous bone graft has been mandatory. Although we feel encouraged by our preliminary results the DCS will have to undergo arduous clinical trial.

Adult↗
PubMed1991

Effects of positive end-expiratory pressure (PEEP) ventilation on the exocrine pancreas in minipigs.

The effect of positive end-expiratory pressure (PEEP) ventilation on the pancreas was studied in 22 anesthetized minipigs. Five pigs were treated with intermittent positive pressure ventilation (IPPV) for 21 h (Group I) and six pigs were treated with a PEEP of 15 cm H2O for 21 h (Group II). We next explored the influence of PEEP ventilation while stimulating the pancreas with Ceruletide, a synthetic cholecystokinin (CCK) analog, at 2.3 micrograms/kg per h.i.v. for 3 h. Ventilation with IPPV for 4 h (n = 5, group III) was compared with PEEP of 15 cm H2O for 4 h (n = 6, group IV). Changes in serum-lipase were observed in all groups. The average lipase level rose from 12.6 U/l to 67 U/l group I and from 15.1 U/l to 129.2 U/l in group II (P = 0.025 for group I vs group II). In group IV (PEEP and Ceruletide), the mean lipase level rose about six times more than in group III (IPPV and Ceruletide). The difference was significant (P less than 0.00005). By three-factor ANOVA analysis, effects due to the drug (P less than 0.000006) and to PEEP (P less than 0.038) as well as a threefold interaction could be demonstrated, i.e., using Ceruletide a greater PEEP effect on lipase than without the drug (P less than 0.023) took place. There were no significant histological changes of the pancreas in groups I and III. In group II (21 h PEEP), vacuolization of acinar cells was evident; on the ultrastructural level, indication was given that these vacuoles derived both from the Golgi apparatus and from fusion of individual zymogen granules. In group IV (PEEP and Ceruletide), the focal appearance of fatty-tissue necrosis and acinar cells as well as hemorrhage of the gland was observed. We conclude that PEEP ventilation impairs the function of the pancreas and produces even more deleterious effects when the gland is stimulated. These effects should be considered when PEEP is used in clinical practice in the treatment of acute pancreatitis.

Amylases↗
PubMed1990

[Malignant teratoma of the thyroid gland].

Two years after a bilateral subtotal thyroidectomy because of nodular goitre a 45-year-old woman noticed a firm nodule on the right side of her neck. Because malignant cells were suspected on cytological examination of a fine-needle biopsy, thyroidectomy was performed which revealed an immature malignant teratoma of the thyroid. A 4 cm local recurrence was noted by computed tomography only three weeks later. Despite chemotherapy (four cycles of vinblastine, cisplatin and bleomycin), radiotherapy to the neck (total dose of 60 Gy) and surgical excision of the infiltrated sternocleidomastoid muscle, bone, liver and lung metastases occurred within 10 months. Four weeks later the patient died of global cardiac failure and tumour cachexia.

Adult↗
PubMed1990

[Luxations in the sternoclavicular joint].

Primary open reduction and fixation with Kirschner wires and/or fascia lata or PDS is recommended in the treatment of acute traumatic luxations of the sternoclavicular joint. If not bent or secured against migration, K-wires may be the reason for lethal postoperative complications. We report on 7 own patients with luxation of the sternoclavicular joint and discuss the problems in diagnosis, operative and postoperative treatment of this rare lesion.

Adult↗
PubMed1989

Lysolecithin concentration in pancreatic tissue during therapy with phospholipase A2-inhibitors in acute necrotizing pancreatitis.

Four hours after hemorrhagic necrotizing pancreatitis was induced in experimental animals the concentration of lysolecithin in pancreatic tissue rose (p = 0.0025). Parenteral administration of unspecific inhibitors of phospholipase A2, such as chlorpromazine, gabexat mesilat and CaNa2 EDTA, can neither inhibit elevation of the enzyme nor influence the course of the disease.

Acute Disease↗
PubMed1989

[Fibrolamellar liver cell cancer. A case report].

Fibrolamellar hepatocellular carcinoma of the liver is a rare variant of hepatocellular carcinoma with characteristic morphological patterns and a good prognosis. Preoperatively the tumor is rarely diagnosed. Surgical treatment is resection, hemihepatectomy or transplantation of the liver. We report a case of a 51 years old patient with fibrolamellar hepatocellular carcinoma of the liver.

Carcinoma, Hepatocellular↗
PubMed1988

[Alpha-mercaptopropionylglycine in the experimental hemorrhagic shock animal model].

Oxygen radicals may have an important role in the shock process. Several studies showed that stimulated polymorphological granulocytes altered biological membranes to a varying degree, especially in the pulmonary circulation. It could be demonstrated on isolated mitochondria that substances of the thiol group carrying SH-groups are capable of counteracting this damaging influence of toxic O2-metabolites. Furthermore the use of the thiol reagents was associated with an improvement in the mitochondrial energy situation (adenosine triphosphate increase). A total of 12 dogs were included into the study. Using standardised conditions haemorrhagic shock was induced by controlled bleeding. After reaching a mean blood pressure of 40 mmHg and a stabilisation of this stage, alternatively either 250 mg of alpha-mercaptopropionylglycine (therapy group T) or an equivalent volume of physiologic saline (controls K) were administered at random. In the dogs on controlled respiration the effect of the above-mentioned substance upon the haemodynamic of the total organism was monitored by means of continuous measuring of the arterial pressure by a Swan-Ganz catheter. Furthermore the acid-base balance, the electrolytes sodium, potassium and calcium were analyzed. Whereas the sodium and potassium values as well as the acid-base balance did not show any difference between both groups, the haemodynamic parameters partly showed very distinct differences, however, without being statistically significant in most cases. The administration of the thiol reagent (group T) led to a reduction in mean arterial pressure, heart rate, peripheral resistance and rate-pressure product. As far as the survival rate at the end of the study was concerned, no difference between the control and therapy group was seen.(ABSTRACT TRUNCATED AT 250 WORDS)

Acid-Base Equilibrium↗
PubMed1988

[Malignant hyperthermia. An unusual course of a rare disease].

An account is given of unusual course of a hyperthermic crisis in a 23-year-old male who underwent repeated anesthetics. As yet little has been reported about Isoflurane, which we presume to have been the triggering agent. In this case only the surgically untreated lower extremity developed rigor, with which malignant hyperthermia is associated, whereas the surgically treated extremity, where circulation had been stopped with a tourniquet, remained unaffected. Rigor and contracture of the affected extremity were so severe that they led to a compartment syndrome, necessitating fasciotomy. No observation of this kind has been published before. In addition to a discussion of this dissociated effect in malignant hyperthermia, a detailed account of the course of the crisis is given.

Adult↗
PubMed1985

[Phospholipase A2 inhibitors in hemorrhagic-necrotizing pancreatitis. Animal experiment evaluation of a new treatment concept].

In mongrel dogs with induced pancreatitis we studied the effects of the nonspecific phospholipase A2-inhibitors chlorpromazine, gabexate mesilate and CaNa2EDTA on the course of pancreatogenic shock and the intrapancreatic lysolecithin production. The local level of lysolecithine the metabolite of phospholipase A2 was lower in the treated than in the untreated control groups. Gabexate mesilate was the most active inhibitor. However, none of the drugs had a beneficial influence on hemodynamics, chlorpromazine made it even worse. Morphologically no difference was seen between the treated and untreated groups.

Acute Disease↗