PubMed Health⌕ Search

Biomedical subjects

M Katsumi

Publications and source records attributed to M Katsumi.

At least 19 recordsLinked to original sources
PubMed1997

Bacterial isolation from slaughtered pigs associated with endocarditis, especially the isolation of Streptococcus suis.

Bacterial isolation from slaughtered pigs with endocarditis was carried out from 1985 to 1994. A total of 495 (0.025%) out of 2,006,127 pigs were diagnosed as having endocarditis. Though bacteria were significantly isolated from 399 of the 495 pigs, bacteria could not be isolated in 96 pigs (19.4%). In 11 pigs, 2 bacterial species were isolated from heart lesion. Streptococcus suis was isolated from 127 cases (25.7%), Streptococcus dysgalactiae from 75 (15.2%), Erysipelothrix rhusiopathiae from 63 (12.7%), Actinomyces pyogenes from 39 (7.9%), Pasteurella multocida from 11 (2.2%). Staphylococcus aureus from 10 (2.0%), and Streptococcus porcinus from 8 (1.6%). Among the 99 isolates biochemically identified as S. suis, the major serotype was S. suis type 2 (35.4%). The remainder of the typable isolates were identified as serotypes 1/2 (2.0%) and 9 (1.0%). A total of 61 isolates (61.6%) were untypable.

Abattoirs↗
PubMed1996

Comparative preparation methods of sialylated capsule antigen from Streptococcus suis type 2 with type specific antigenicity.

The capsular polysaccharide (CPS) of Streptococcus (S.) suis type 2 was isolated from a type strain of S. suis NCTC 10234 by three different preparative methods: (A) lysozyme treatment method, (B) autoclave extraction method, and (C) HCl-extraction method. The structural characteristics of the three CPS (CPS-A, B and C) were examined by gel permeation chromatography, reactivity against rabbit antiserum and proton-nuclear magnetic resonance (1H-NMR). N-Acetylneuraminic acid (NeuAc) residues as sialic acid in CPS-C were partially dissociated or degraded during preparation with a remarkable decrease in the molecular mass and the antigen activity. Although both methods A and B produced intact CPS without releasing NeuAc residues, method B was considered to be a more suitable procedure for preparing the CPS antigen because of time-saving and safety factors. Sugar analysis by high performance liquid chromatography and gas liquid chromatography showed that CPS-B consisted of five kinds of sugars: Rhamnose (Rha), Glucose (Glc). Galactose (Gal), N-acetylglucosamine (GlcNAc) and NeuAc, in a molar ratio of 1.00:0.95:3.68:0.80:1.31. After complete removal of NeuAc residues by mild acid hydrolysis of CPS-B, the reactivity with anti-type 2 serum was not detected. The NeuAc residue in CPS of S. suis type 2 strain was thought to be the antigen epitope portion.

Antigens, Bacterial↗
PubMed1991

Typing of Rhodococcus equi isolated from submaxillary lymph nodes of pigs in Japan.

Isolation of Rhodococcus equi from the submaxillary lymph nodes of pigs, with or without caseous lymphadenitis, and typing of the isolates by two serological methods were carried out. The rate of isolation of the organisms from the lymph nodes of pigs was 5 times higher in the lymph nodes with caseous lymphadenitis than in those without the lesion. Of 219 isolates, 146 (66.7%) were typable by the method of Prescott, while all the 219 isolates (100%) were typable by the method of Nakazawa et al. The most frequently isolated were serotype 2 of Prescott (identical to serogroup 16 of Nakazawa et al.), and serogroup 3 of Nakazawa et al., which did not correspond with any serotypes of Prescott. Serotypes/serogroups of R. equi from pigs were thus first clarified in Japan.

Actinomycetales Infections↗
PubMed1989

[Clinical effect of combined chemotherapy with UFT, MMC and OK-432 in recurring or advanced cancer of the digestive organs].

The usefulness of UFTMO therapy (combined chemotherapy with UFT, MMC and OK-432) performed in 40 cases of recurring or advanced cancer of the digestive organs was investigated. According the response criteria by Koyama et al., of 40 eligible cases, the treatment was judged effective in 13, 2 CR and 11 PR cases with a response rate of 32.5%, while of the 35 complete cases, 2 CR and 9 PR cases made for 11 effective cases and a response rate of 31.4%. Side effects were observed in 58.3% of the 36 evaluated cases; of the subjective and objective side effects, however, none were serious enough to require cessation of administration, while stopping administration in the cases of abnormal laboratory findings resulted in rapid recovery. UFTMO therapy, therefore, is considered to be one of the beneficial treatments for recurring or advanced cancer of the digestive organs.

Adult↗
PubMed1987

Induction of activated natural killer cells from murine spleen cells primed in vivo and subsequently challenged in vitro with the streptococcal preparation OK432.

The present study shows that natural killer cell-mediated cytotoxicity of BALB/c mouse spleen cells to syngeneic tumor cells was augmented by in vivo priming or in vitro stimulation with the streptococcal preparation OK432. The augmentation of spleen cell cytotoxicity to syngeneic tumor cells by in vivo priming alone with OK432 was lower than that obtained by in vitro stimulation alone with OK432. When the murine spleen cells primed in vivo with OK432 were rechallenged in vitro with OK432 at various intervals, the natural cytotoxicity was more strongly enhanced than that seen with in vitro stimulation alone. The cell surface phenotype of killer cells activated with OK432 was Thy 1+ and asialo GM1+, suggesting the activated natural killer cell. Next, mice were transplanted with syngeneic colon adenocarcinoma cells, and primed in vivo with OK432. These spleen cells were subsequently challenged in vitro with OK432. These spleen cells displayed a strong cytotoxic activity not only to the transplanted adenocarcinoma cells but also to other syngeneic tumor cells.

Adenocarcinoma↗
PubMed1987

[5-FU concentration in tumor tissue and the antitumor effect in patients with gastric cancer after oral administration of UFT].

Serum and tumor tissue concentration of FT, 5-FU and uracil were measured in 23 patients with gastric cancer who were administered UFT preoperatively. The degeneration of cancer cells was evaluated histologically and the correlation between 5-FU concentration in the tumor tissue and the antitumor effect was assessed. The average concentration of 5-FU in tumor tissue (0.122 microgram/g) was significantly higher than that in normal gastric tissues. Serum 5-FU concentration was very low, suggesting no accumulation of 5-FU in blood. A positive correlation between the concentration of 5-FU and uracil in tumor tissues was found. A 5-FU level higher than 0.05 microgram/g was frequently demonstrated in tumor tissue, resulting in moderate degeneration of cancer cells in many cases. When tumors were classified according to histological type, intratumoral 5-FU concentration was not always correlated with degeneration of cancer cells. The above results suggested that UFT was a very effective drug for stomach cancer because of high tumor affinity for 5-FU, but that it is necessary to consider the sensitivity of tumor cells to antitumor drugs in order to obtain an excellent antitumor effect.

Administration, Oral↗
PubMed1985

Microwave tissue coagulation applied clinically in endoscopic surgery.

On the basis of the ample basic knowledge acquired by repeated experiments, we applied our microwave tissue coagulator in endoscopic surgery, in a total of 59 patients with benign and malignant lesions encountered during a period of two years beginning in July, 1981. Hemostasis was achieved in 96.5% of all the cases with bleeding lesions in the digestive tract. Stenosis was alleviated in 86% of the cases with esophageal or rectal stenosis. Furthermore, the technique was successfully used for hemostasis and tumor reduction in inoperable early cancer cases. Our device is unique in that the electrode is thrust into tissue, which assures us of a satisfactory result. In this point, it must be clearly distinguished from an electrocoagulator or a laser coagulator, which is associated with a risk of injuring intact tissue. The range of coagulation is adjustable by changing the length of the monopolar antenna and electric output, and by employing a coaxial cable of appropriate thickness. In conclusion, our microwave tissue coagulator can be used easily and safely in clinical endoscopic surgery.

Adult↗
PubMed1985

[Analysis of intestinal motility by enteric sound recording system--clinical evaluation of aclatonium napadisilate and prostaglandine F2 alpha on intestinal movement].

In patients with so-called postoperative enteroplegia after laparotomy, effects of aclatonium napadisilate (abbreviated as TM-723 hereinafter) and prostaglandine F2 alpha (abbreviated as PGF2 alpha) on intestinal motility were determined using an enteric sound recording system (phonointestinography) and simultaneously the time required for breaking wind after operation was examined. TM-723 was administered at a dose of 50 mg from the intestinal fistula after dissolved in 5 ml of physiological saline, while PGF2 alpha was administered as an i.v. drip infusion at a dose of 2 mg dissolved in 500 ml of 5% glucose solution. Intestinal movement was determined at McBurney point using the enteric sound recording system developed at our department. The time required for breaking wind after operation was determined in three groups received TM-723, PGF2 alpha and placebo, respectively. No significant intergroup difference was observed. As to effect on intestinal motility, TM-723 increased intestinal motility when it was reduced in so-called postoperative enteroplegia phase. Its efficacy became higher with a decrease in motility. On the contrary, TM-723 rather tended to depress intestinal motility when it was increased. On the other hand, PGF2 alpha tended to increase intestinal motility regardless of motility in enteroplegia phase.

Acetylcholine↗
PubMed1985

[Study on the function of the sphincter of Oddi in patients with gallstone disease by dynamic hepatobiliary scintigraphy].

By using hepatobiliary scintigraphy, function of the sphincter of Oddi in 88 patients undergoing cholecystectomy with or without common bile duct exploration and T-tube drainage for gallstone disease was studied. These patients consisted of three groups; 57 patients with gallbladder stones (Group I), 25 with common duct stones migrated from the gallbladder (Group II) and 6 with primary common duct stones (Group III). Time-activity curves (T-A curve) obtained from the region of terminal end of the common bile duct were classified into 4 types which were different from each other. On the T-A curve, the rate of decrease (ROD, %) was defined as the difference between the count rate in the maximum and that at the 20 min after the maximum. The ROD in Group I was significantly greater than that in Group II or III (p less than 0.01). The ROD in Group II was also significantly greater than that in Group III (p less than 0.05). The increase of ROD in Group III after cholecystokinin-pancreozymin administration was less remarkable as compared with that in Group II. Thus, we conclude that the function of the sphincter of Oddi in these three groups may be different from each other.

Ampulla of Vater↗