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M Kemali

Publications and source records attributed to M Kemali.

At least 19 recordsLinked to original sources
PubMed2000

Inelastic neutron scattering measurements and Ab initio calculations of hydrogen in single-crystal palladium

Using a single-crystal sample of PdH, we have measured the inelastic form factor for exciting the proton from its ground state to various excited states as a function of crystal orientation. Because of the anharmonicity of the potential well which splits otherwise degenerate states, each inelastic form factor could be measured individually. Agreement between theory and experiment is excellent, both with regard to the transition energies and to the modulation of the intensity, giving confidence in the quantum treatment of the proton and hence in the use of the model to predict other properties of the system.

Journal Article↗
PubMed1990

The asymmetry of the habenular nuclei of female and male frogs in spring and in winter.

Male and female frog brains stained according to the Nissl method and cut transversely, 15 microns thick, at the level of the habenular nuclei, were investigated in spring and winter. Right and left habenular nuclei were examined. The volume and the standard deviations were calculated in each portion of the habenular nuclei investigated. The frog habenula consists of a single cell group: one on the right and two on the left side of the brain which differ, among themselves, both in the volumes of the neuropil and of the cellular ring. Functional corollaries of this striking asymmetry are still unknown. However, female and male frogs' habenular nuclei are longer and larger in spring--when frogs are sexually active--than in winter. We propose that structural brain asymmetries may be sex linked and may be induced by steroid hormonal effect in the central nervous system.

Animals↗
PubMed1990

Neuroelectrophysiology of the morphologically asymmetric habenulae of the frog.

1. Electrophysiological recordings of the spontaneous and evoked activities of the frog habenular nuclei are presented. 2. The traces were recorded simultaneously from both habenular nuclei and an histological test was performed in order to show the exact location where the electrodes were inserted in the brain for the purpose of making the recordings. 3. The traces show a dissimilarity which agrees with the morphological asymmetry of these nuclei demonstrated histologically.

Action Potentials↗
PubMed1990

Superficial and deep blood vessel distribution in the frog telencephalon. Reference to morphological brain asymmetries.

Nine frogs of the species "Rana esculenta" were heart perfused with Microfile Silicone Rubber. The frogs were examined both after dissection (cut with a razor blade) to study the superficial blood vessel pattern, and histologically (the Nissl staining method) to study the distribution of the deep blood capillaries. While the superficial blood vary in pattern, the deep capillaries are distributed symmetrically. This finding does not support a correlation between blood vessel pattern and morphological brain asymmetry, at least in the frog, and thus other explanations must be sought to explain brain asymmetry.

Animals↗
PubMed1989

Lithium decreases retinal melatonin levels in the frog.

The effect of the acute i.p. administration of lithium chloride (1 mg/kg) and of haloperidol (1 mg/kg) on retinal melatonin levels was studied in light-adapted and dark-adapted frogs of the species Rana esculenta. Two hours following drug administration, animals were killed by decapitation and a single retina from each frog was collected and homogenized in 1 ml of chilled 0.1 N HCl. After centrifugation, the pH of the supernatant was adjusted to 7.0. Melatonin was extracted by diethylether and assayed by double antibody radioimmunoassay (RIA). Lithium induced a significant decrease of retinal melatonin levels both in light-adapted (P less than 0.006) and in dark-adapted (P less than 0.01) animals, whereas no change was observed after haloperidol treatment. These results suggest that the scleral aggregation of pigment granules induced by lithium in the frog retina is not mediated by a stimulation of melatonin synthesis.

Animals↗
PubMed1989

Substance P and hydra: an immunohistochemical and physiological study.

1. The distribution of substance P-like immunoreactivity was studied in Hydra attenuata using the peroxidase-antiperoxidase technique. 2. Positive immunoreactivity was observed in ectodermal nerve cells and fibers as well as in nematoblasts at various stages of differentiation. 3. Administration of synthetic substance P to regenerating hydra did not affect regeneration rates. Exogenous substance P administration stimulated tentacle contraction and nematocyst displacement within battery cells. 4. It is suggested that substance P acts on the contractile apparatus of Hydra tissues.

Animals↗
PubMed1987

A horseradish peroxidase study of the olfactory system of the frog, Rana esculenta.

The olfactory system of the frog Rana esculenta was studied by using horseradish peroxidase (HRP) tracing of axonal pathways. Injections of HRP were made in the main olfactory bulb (MOB), accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), the amygdala (AMY), and in a zone of the leteral wall of the telencephalic hemisphere immediately posterior to the AOB. Projections from these sites are described and are generally similar to those obtained by degeneration methods. However, HRP reveals more extensive olfactory connections than previously reported. Ipsilateral, contralateral, and bilateral projections are described. The MOB, AOB, and AON have ipsilateral connections to each other. The MOB and AOB have very different projections. The MOB and AON project via the habenular commissure (HC) to the contralateral medial wall of the telencephalon. Ipsilateral MOB fibers also terminate in this cell-free zone where the medial forebrain bundle (MFB) originates. The AOB projects to the lateral cortex of the contralateral telencephalic hemisphere via the HC and also to the ipsilateral AMY and lateral forebrain bundle (LFB) from where some fibers project contralaterally. HRP injections in the AMY retrogradely fill cells in the ipsilateral AOB, two nuclei of the ipsilateral hypothalamus and a nucleus of cells caudal to the ipsilateral nucleus isthmi. Fibers are also labeled that project to the contralateral AMY. Few fibers were observed to decussate in the interpeduncular nucleus or optic chiasma. No olfactory fibers were found to project to the habenular nuclei, and no labeled neurons were found to project to the olfactory bulbs. No morphological asymmetry was observed qualitatively in the distribution of olfactory fibers in the two halves of the brain.

Amygdala↗
PubMed1987

Frog retinal pigment screening and lithium.

The effect of the intraperitoneal injection of lithium on the pattern of the pigment screening (PS) of the frog retina has been studied in various illumination conditions. Lithium enhances the PS pattern induced by dark and it substantially modifies the PS pattern induced by the other light conditions. The dark-induced PS pattern is very stable and might be regulated by a single factor. The PS of light-adapted frogs is easily modified by drug administration and probably depends on a variety of factors which may be affected differently by the various injected substances. The possibility that lithium, melatonin and darkness might act in the same way or on the same system which regulates the PS in dark-adaptation must not be disregarded.

Animals↗
PubMed1987

Lithium and melatonin: morphological modifications induced in frog retina pigment screening.

Pigment screening (PS) is a phenomenon occurring in the retina of lower vertebrates, which consists in the dispersion, induced by the light, of melatonin granules into processes of the pigment epithelium which extend between photoreceptors, and in the aggregation, induced by the dark, of the same granules within the cell bodies of the pigment epithelium. It has been hypothesized that this phenomenon might be modulated by melatonin, which is present not only in the pineal but also in the retina, and whose retinal content is high in the dark and low in the light. In the present study we demonstrate that a single intraperitoneal injection of lithium chloride affects in a peculiar way PS of light-adapted frogs and of frogs subjected to natural day/night conditions, but not that of dark-adapted frogs. The implications of this findings are briefly discussed, also in view of the reported effect of lithium on the 24-hour rhythm of retinal melatonin, and of the possible relationship between this effect and the altering properties of lithium on other biological rhythms.

Adaptation, Ocular↗
PubMed1985

Substance P-like immunoreactivity in the frog dorsal root ganglia.

The distribution of substance P-like immunoreactivity was studied in the thoracic dorsal root ganglia of the frog Rana esculenta by immunohistochemistry. Substance P-like immunoreactivity was contained in approximately 50% of primary sensory neurons. The immunoreactive fibers arising from the cell bodies are collected in small bundles within the ganglia neuropil before entering the central and peripheral roots.

Animals↗
PubMed1985

Cobalt injected into the right and left fasciculi retroflexes clarifies the organization of this pathway.

A cobaltic lysine complex was injected separately into the right and left fasciculi retroflexes of the frog. This tracing technique labeled, in a Golgi-like manner, the neurons which initiate the fasciculi retroflexes and revealed details of their morphological pattern. The fasciculi retroflexes originate from various neurons distributed in the diencephalon and mesencephalon, but their main source is the habenular nuclei. In the frog there are dorsal and ventral habenulae which are homologous to the medial and lateral habenulae, respectively, of mammals. In the frog the dorsal habenulae are strikingly asymmetric. Our study shows that the fasciculus retroflexus is composed of several compact separated bundles of fibers and that the fibers originating from the dorsal habenular nuclei project to the interpeduncular nucleus, while those originating from the ventral habenular nuclei project beyond the interpeduncular nucleus, but so far we have not been able to show the exact site of their termination. The labeling of cells within the interpeduncular nucleus raises the possibility that the habenulo-interpeduncular tract is reciprocal in function. The finds support our previously reported hypothesis on the theoretical interpretation of the functional circuitry of the frog habenulo-interpeduncular system.

Animals↗
PubMed1984

Drugs and the frog retina. Effect of dopaminergic agents on the pigment screening of light- and dark-adapted frogs.

This is a study, using the light and electron microscope, of the action of a dopamine agonist (apomorphine) and of a dopamine antagonist (haloperidol) on the retinal pigment screening (PS) of light- and dark-adapted frogs. Pigment screening is a phenomenon which consists of the migration of melanin granules into processes of the pigment epithelium that extend between photoreceptors, in response to changes in the conditions of illumination. In the light the pigment migrates vitreally , in the dark it aggregates sclerally . A single intravenous injection of apomorphine (0.15 mg/kg) and of haloperidol (1 mg/kg) did not induce substantial modifications in the pattern of pigment screening which was similar to that of controls both in light- and dark-adapted frogs. This suggests that dopamine is probably not directly involved in the phenomenon of pigment screening in the retina of the frog.

Animals↗
PubMed1984

The distribution of substance P in the habenulo-interpeduncular system of the frog shown by an immunohistochemical method.

The distribution of substance P(SP)-like immunoreactivity was studied in the habenulo-interpeduncular (HAB-ITP) complex of the frog Rana esculenta by means of the immunohistochemical peroxidase-antiperoxidase (PAP) method. The HAB of the frog were subdivided into dorsal and ventral nuclei, corresponding to the medial and lateral HAB of mammals respectively. In addition, the dorsal HAB of the frog were morphologically asymmetric, since only the left has a lateral and a medial subnucleus. SP-like immunoreactive cells were found in the right dorsal HAB, in the lateral subnucleus of the left dorsal HAB and in a nucleus of scattered cells located rostrodorsally to the ITP. However, the medial subnucleus of the left dorsal HAB and a portion of the right dorsal HAB facing the 3rd ventricle, did not show SP immunoreactivity. Immunoreactive fibers were found in the medial subnucleus of the left dorsal HAB, in the two fasciculi retroflexes and in the ITP. Moreover, immunoreactive terminals were observed in the ventral HAB of both sides, in the ITP and on the blood vessels which cross the ITP. It is assumed that a portion of fibers connecting the various structures of the HAB-ITP complex belongs to the SP peptidergic system as suggested for mammals. However, in contrast to the mammals, the results on the frog are in favour of an histochemical asymmetry of the SP-like immunoreactivity, since a different distribution of this peptide was found between the right and left side of the brain. Such histochemical asymmetry was less pronounced than the morphological asymmetry and its functional meaning is unknown.

Animals↗
PubMed1983

Neuroanatomical asymmetry in the telencephalic hemispheres of the frog Rana esculenta.

The frog's brain postfixed with osmium tetroxide and stained with uranyl acetate was examined for asymmetries. Examination with the light microscope of serial semithin sections of the telencephalon revealed a nucleus of dark cells located in the lateral cortex of only the left hemisphere in a region close to the 'porcion arqueada' of Pedro Ramón y Cajal. Electron microscopy of ultrathin sections suggests that the cells of this asymmetric nucleus are neurons with a coarse cytoplasmic texture, where large inclusions occur, and with broad cisternae of endoplasmic reticulum.

Animals↗