Biomedical subjects

M Kinbara

Publications and source records attributed to M Kinbara.

5 recordsLinked to original sources

PubMed1992

Expression of peroxidase activity in rat tracheal epithelial cells associated with Mycoplasma pulmonis.

Endogenous peroxidase activity has not been localized in the tracheal mucosal epithelial cells of specific pathogen-free (SPF) rats. After natural infection with Mycoplasma pulmonis in SPF rats, peroxidase activity became localized to the endoplasmic reticulum, nuclear envelope, and Golgi apparatus of tracheal ciliated or mucous secretory cells. Some secretory cells occasionally had peroxidase-positive secretory granules. At 1 wk M. pulmonis was found to attach to these epithelial cells, which then showed positive peroxidase activity at 2 wk. Serum antibody titers against M. pulmonis were positive at 5 wk. These results suggest that virulent mycoplasma infection and interaction with the tracheal epithelial cells trigger the de novo expression of peroxidase activity, which seems to play a role in mucosal anti-microbial defense mechanisms.

Animals↗

PubMed1990

Do the cardiac glands exist? 6. The cat.

We performed histological and histochemical investigations on so-called cardiac glands and gastric glands in the cat to clarify the existence of the former. 1. Cardiac glands possess some parietal cells and strongly PAS-positive glandular cells in the base of glands containing a small number of fine pepsinogen granules. Consequently, we consider them to be undifferentiated gastric glands. Therefore, the cardiac glands do not exist in the cat. 2. Glandular cells in the basal part of undifferentiated gastric glands react negatively or very weakly to AB (pH 2.5 and 0.5) and present a red color with PAS-AB (pH 2.5) stain. 3. A large number of strongly PAS-positive and pepsinogen granule containing cells are distributed not only in the glandular body but also in the basal part of the glands. These cells are immature chief cells. 4. The above-mentioned cells react weakly or moderately to AB (pH 2.5 and 0.5) and stain light purple by PAS-AB (pH 2.5). They possess weak and strong acid mucopolysaccharides and sialomucin. 5. Mature chief cells are distributed mainly in the glandular base. They are PAS-negative, react moderately or strongly to AB (pH 2.5 and 0.5), and present a dark color of AB with PAS-AB (pH 2.5) stain. Consequently, it can be concluded that these cells contain weak and strong acid mucopolysaccharides but no sialomucin. 6. Mature chief cells possess no sialomucin. However, they present stronger reactions to AB (pH 2.5 and 0.5) than those in immature chief cells. This characteristic does not appear in man and many other types of mammals studied.

Animals↗

PubMed1989

Does the polystomatic gland exist?

According to the P.N.A., the N.A.J. and some scholars, the sublingual gland has the ductus sublingualis major and ductus sublinguales minores. This means that the gland is a polystomatic gland. We intended to determine whether the so-called polystomatic gland exists or not. 1. According to the P.N.A., the N.A.J. and some scholars, the gl. sublingualis has the ductus sublingualis major and ductus sublinguales minores. This means the gland is a polystomatic gland. However, the formation of one gland with plural excretory ducts is embryologically impossible, in other words, the polystomatic gland does not exist. 2. Many scholars described that the gl. sublingualis was composed of the gl. sublingualis major and g11. sublinguales minores. However, they are completely different kinds of glands. Accordingly, we suggest the terms for these glands: the g1. sublingualis and its ductus sublingualis ("major" is useless), the g11. sublinguales minores and their ductus sublinguales minores. 3. The N.A.V.J. and some scholars use the term g1. sublingualis polystomatica or parvicanalaris. However, this is a group of a number of independent glands each of which has its own excretory duct. Such a gland should not be regarded as a single gland. We suggest that the term g11. sublinguales minores and their excretory ducts should be replaced with the term the ductus sublinguales minores. 4. The g1. lingualis anterior, g1. retromolaris and g1. lacrimalis are not single glands but a group of several independent glands each of which has its own excretory duct. Accordingly, they should be termed the g11. linguales anteriores, g11. retromolares and g11. lacrimales such as the g11. labiales, g11. buccales and g11. palatinae.

Animals↗

PubMed1989

A new differentiation type of the chief cells in the cat gastric gland.

We demonstrated a new differentiation type of chief cell in the cat gastric gland. 1. Numerous PAS-positive cells were distributed not only in the glandular body but also in the glandular base. 2. These cells, namely immature chief cells, contained a large number of claret-colored fine pepsinogen granules. 3. Immature chief cells did not exist in the glandular base of man and many other types of animals. 4. The PAS-positive cells in the glandular base gradually decreased in PAS-positive substance and changed into PAS-negative cells with a reticulate framework. These mature chief cells contained numerous dark blue coarse pepsinogen granules. 5. The mature chief cells reacted moderately to AB (pH 2.5), and weakly to AB (pH 0.5), while staining with PAS-AB (pH 2.5) produced a moderate to strong color reaction of AB. Such reactions were characteristic to these cells and could not be found in man or many other types of animals studied. 6. PAS-positive cells i.e. the immature chief cells, reacted strongly to AB (pH 2.5), and moderately to AB (pH 0.5), while staining with PAS-AB (pH 2.5) produced a violet color. 7. The above-mentioned reactions were the same in mucous neck cells with mean undifferentiated characteristics. Such cells do not exist in the glandular base of man and many other types of animals. 8. The glandular base is generally occupied only by mature chief cells, though a large number of undifferentiated cells or immature chief cells were distributed in the same area of the cat.(ABSTRACT TRUNCATED AT 250 WORDS)

Aminosalicylic Acid↗

PubMed1984

[Experimental studies on oral administration of nitrosourea anti-tumor agent, MCNU].

Experimental studies with orally administered MCNU, a water-soluble nitrosourea, yielded the following results. MCNU produced a significant increase in life span, and 60-day survivors were observed by various schedules in L1210 leukemia. The therapeutic ratios of MCNU were almost similar to those of CCNU. With Lewis lung carcinoma and Ehrlich ascites carcinoma implanted into the stomach wall, its antitumor activity by oral administration was slightly more effective than by intravenous route. In Beagle dogs, hematologic toxicity and gastrointestinal toxicity (vomiting, diarrhea) were noted by oral administration, similar to intravenous administration, but its toxicity was mild. The maximum blood level of MCNU was noted at 30 minutes after oral administration in Beagle dogs. The half life (23.7 min) by oral administration was similar to that by intravenous route. From these results, the oral administration of MCNU deserves the consideration as a form of treatment now given other MCNU routes.

Administration, Oral↗