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M Klencki

Publications and source records attributed to M Klencki.

12 recordsLinked to original sources
PubMed1997

Multifarious system for quantitative analysis of histologic compartments.

A system is presented for measuring relative volumes of histologic compartments in microscopic slides. The computer program for that system was written by us as an MS windows 3.x application. The applied method of measurements, based on the "point-counting" technique, requires the recognition of the compartments, corresponding to the selected points of an image. The neural network was employed to facilitate the process of classification of the examined points. Nine descriptors of an image were selected as an input to the network (a pattern). Error-free compartment recognition was not always achieved because of some overlapping of the pattern classes. Nevertheless, the semi-automation of the assignment of the examined points to histologic compartments makes the measurements less laborious and time-consuming.

Animals↗
PubMed1994

Interactions between atrial natriuretic factor (ANF) and thyrotropin or somatostatin in their effects on thyroid growth processes; studies in vitro and ex vivo in vitro.

The goal of our present study has been to examine the effects of the atrial natriuretic factor (ANF) on the growth processes in rat thyroid lobes. In the initial in vitro experiment, thyroid lobes were preincubated with rat ANF (Sigma) for 30 min in RPMI 1640 medium with 3H-thymidine (2 microCi/ml), and later on 15% fetal calf serum (FCS), Hepes buffer and the remaining tested substances [TSH 20 mIU/ml, somatostatin (SS) 10(-7)M] were added. Preincubations with ANF were not conducted in the controls and in the group exposed to TSH alone. Incubations of all the examined groups (controls, TSH alone, ANF alone, ANF together with TSH or ANF together with SS) with 3H-thymidine were carried out for 4 hours. We obtained the following results: at none of the examined concentrations (10(-5)M, 10(-7)M, 10(-9)M), did ANF significantly affect the rate of 3H-thymidine incorporation in vitro. Neither did TSH alone nor ANF with TSH jointly significantly influence the process in question. However, we observed increased rates of the 3H-thymidine uptake, following the joint exposure of thyroid lobes to ANF (10(-7)M or 10(-9)M) and SS (10(-7)M), when compared to ANF alone. In the ex vivo in vitro experiment, direct intrathyroidal microinjections of ANF alone or jointly with TSH or SS, were carried out. Twenty four (24) hours after the microinjections, all the animals were sacrificed by decapitation, the thyroid lobes being collected and incubated for 4 hours with 3H-thymidine (2 microCi/ml).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1994

Melatonin-induced decrease of the size of thyrocyte nuclei in rat thyroids incubated in vitro.

The mean volume, intersection area and perimeter of thyrocyte nuclei in rat thyroid lobes incubated with melatonin (Mel), thyrotropin (TSH) or with both substances together, were investigated. The measurements were carried out using a computer system for karyometric examination. Melatonin in concentrations of 10(-8) M, 10(-10) M and 10(-12) M significantly decreased the mean nuclear volume and intersection area of thyrocytes (p < 0.01); the mean perimeter of thyrocyte nuclei was significantly decreased by melatonin in concentrations of 10(-10) M and 10(-12) M (p < 0.01). TSH (20 mIU/ml), when applied alone, significantly decreased the mean volume, intersection area and perimeter of thyrocyte nuclei (p < 0.01). TSH generally did not counteract the melatonin-induced decrease of thyrocyte nuclei except for 10(-10) M melatonin concentration when TSH increased the mean nuclear perimeter (p < 0.01) and slightly enlarged the mean volume (p < 0.05), compared with Mel alone. The results suggest an inhibitory influence of melatonin on thyroid follicular cell activity, and are in compliance with earlier observations.

Animals↗
PubMed1993

The usefulness of computer-aided karyometric examination in preoperative differentiation of follicular neoplasms of the thyroid gland.

Fine-needle aspiration biopsy (FNAB) is the most important procedure in preoperative diagnosis of thyroid neoplasms. However, routine cytological examination is insufficient for differentiation between follicular adenoma and follicular carcinoma. Therefore, we decided to assess the usefulness of karyometric analysis in the examination of aspirates from these neoplasms. Morphometric analysis was performed with the use of the computer system for karyometric measurements--"Karyometry Manager" ver. 1.2. We examined cytological smears derived from 26 patients. In 10 of them a nodular goitre was diagnosed in both cytological and histopathological examinations. In the other 16 patients, "follicular neoplasms" were found in cytological examination. These proved to be follicular adenomas (8 cases) and follicular carcinomas (8 cases) on histopathological examination. The following morphometric parameters were measured in 100 nuclei per smear: volume, intersection area, perimeter, convexity coefficient and shape coefficient. We found that: 1) the mean volume and the mean intersection area of thyrocyte nuclei from follicular carcinomas were significantly (p < 0.001) greater than those of nuclei from adenomas or nodular goitres; 2) the mean perimeter of thyrocyte nuclei from follicular carcinomas was significantly greater than the mean perimeter of thyrocyte nuclei from follicular adenomas (p < 0.025) and nodular goitres (p < 0.001); 3) the mean nuclear area of thyrocytes from follicular adenomas was significantly greater than that of thyrocytes from nodular goitres (p < 0.05). Our results show that karyometric analysis can be useful in cytological differentiation of follicular neoplasms.

Adenocarcinoma, Follicular↗
PubMed1993

Occurrence of goitre in relation to iodine deficiency in school children in Lódź City, the Lódź metropolitan area and in Piotrków, Sieradz, Płock, Włocławek, Radom and Skierniewice Voivodships (Lódź coordinating center).

This paper presents the results of studies performed within the Applied Project of the Ministry of Health and Social Welfare and of the Committee for Scientific Research: "Studies on Iodine Deficiency in Poland", including the school children in Lódź City and seven voivodships of the Central Poland. According to the results, the analyzed regions can be regarded as areas of goitrous endemia of a moderate degree (the goitre was found in 32.7% of examined population); the same regions can also be classified among the regions of mild iodine deficiency (the mean iodide concentration in urine was 64.0 micrograms/l). The obtained results prove the to-date's iodine prophylaxis to be not entirely efficient in the areas of interest, demanding an effective model of iodine complementation in diet to be designed.

Adolescent↗
PubMed1993

[Effect of melatonin on the function of the pituitary-gonadal axis in food restriction states].

The effects of melatonin (25 micrograms s.c. daily in the late afternoon during ten days) on the ovarian morphometry and the gonadotropin secretion were investigated in food-restricted and fed ad libitum rats. It was found that in food-restricted rats melatonin produced the significant decrease of the surface area of the whole ovary and of zona granulosa. Moreover melatonin treatment of food-restricted rats resulted in significant decrease of the relative areas of graafian follicles and zona granulosa estimated in relation to the whole ovary cross-section surface area. On the other hand melatonin did not produced significant alterations of the morphometric indices of the ovary in fed ad libitum rats. Melatonin administration was found to inhibit the effects of gonadotropin release in food-restricted but not in normally fed rats. These findings suggest that food restriction sensitizes the pituitary ovarian axis to antigonadotropic melatonin action.

Animals↗
PubMed1992

Food restriction enhances melatonin effects on the pituitary-gonadal axis in female rats.

The effects of melatonin (25 micrograms s.c. daily in the late afternoon for 10 days) on the ovarian morphometry and the gonadotropin secretion were investigated in food-restricted rats and rats fed ad libitum. In food-restricted rats melatonin produced the significant decrease of the surface area of the ovary and of zona granulosa. Moreover, melatonin treatment of food-restricted rats resulted in significant diminishment of the relative areas of Graafian follicles and zona granulosa estimated in relation to the ovary cross-sectional surface area. On the other hand, melatonin did not produce significant alterations of the morphometric indices of the ovary in rats fed ad libitum. Melatonin administration was found to inhibit the effects of gonadoliberin on gonadotropin release in food-restricted but not in normally-fed rats. These findings suggest that food restriction sensitizes the pituitary-ovarian axis to antigonadotropic melatonin action.

Animals↗
PubMed1992

[Comparative analysis of fine needle aspiration biopsy results performed in the laboratory of thyroid diseases, University School of Medicine in Lodz in 1985-91 with results of postoperative histopathologic examinations].

From 1985 to 1991 there were 5889 fine-needle aspiration biopsies of thyroid performed in our laboratory. 703 cytological diagnoses based on biopsy specimens taken from 679 patients, were compared with the results of postoperative histopathological examinations. There were 14% non-diagnostic biopsies. The statistical analysis was performed considering difficulties in differentiation between follicular adenomas and follicular carcinomas. Difficulties in evaluation of biopsies of cystic lesions were also considered. The results of cytological and histopathological examinations were agreeing with one another in 88% cases. In regard to diagnosis of malignant neoplasms, the sensitivity of the cytological investigation was equal to 63% and the specificity equaled to 90%. While considering detection of papillary carcinomas, the sensitivity was equal to 67%. Our results are in a compliance with the view, that the fine-needle aspiration biopsy is a useful method in a preoperative diagnosis of thyroid lesions.

Biopsy, Needle↗
PubMedDate not supplied

Size of thyrocyte nuclei in aspirates from follicular adenomas: correlation with patients' ages.

OBJECTIVE: To determine whether the size of thyrocyte nuclei in aspirates from follicular adenoma correlates with the age of patients. STUDY DESIGN: The karyometric parameters were evaluated in routine aspirates, obtained from 27 patients with follicular adenoma as diagnosed in postoperative histopathologic examinations. The ages of the patients ranged from 11 to 70 years (36.8 +/- 16.3, x +/- SD). The cytologic examination of all the aspirates before surgery revealed "follicular neoplasms." The karyometric evaluation was performed with Karyometry Manager, version 1.2, an image analysis computer system. RESULTS: Neither the mean nuclear volume, mean nuclear intersection area nor mean nuclear perimeter correlated with the ages of the patients. CONCLUSION: These results suggest that in contrast to nodular goiter, in the cytologic examination of follicular neoplasms, the ages of patients should be disregarded when interpreting the size of thyrocyte nuclei.

Adenoma↗
PubMedDate not supplied

Age-related changes in the size of thyrocyte nuclei in aspirates from nontoxic nodular goiter.

It is believed that the size of thyrocyte nuclei correlates with the age of the patient in both normal thyroid tissue and nodular goiter. Therefore, we examined karyometric parameters in aspirates obtained from 38 patients ranging from 18 to 79 years (48.8 +/- 15.2, mean +/- SD). Routine cytologic evaluation revealed nodular goiters in all the patients. Subsequently, the same smears were analyzed with a computer system for karyometric measurements. The mean volume, intersection area and perimeter of nuclei of thyrocytes from nodular goiters correlated positively with age. The results suggest that the age of the patient should be considered when interpreting thyrocyte nuclear size during cytologic examination.

Adult↗