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Biomedical subjects

M Knob

Publications and source records attributed to M Knob.

15 recordsLinked to original sources
PubMed1980

Creatine kinase and its isoenzymes in dog sera.

Creatine kinase activity was determined in 103 clinically healthy dogs. Normal values of total creatine kinase and its isoenzymes have been established, and our method of electrophoresis is described. The normal range of the total-CK activity in dog was higher than that in man (up to 84 U./l in dog, up to 50 U./l in man). The electrophoretic pattern of isoenzymes was also different. The main isoenzyme found in dog serum was the CK-BB, and there was a minor, but evident band of the CK-MM fraction (up to 50 U./l). The CK-MB fraction in normal serum was not measurable (less than 1 U./l). The presence of the CK-BB fraction in serum of the dog, and the activities of single isoenzymes are discussed.

Animals↗
PubMed1979

[Plasma myoglobin level as a course criterium in patients with acute myocardial infarct].

Myoglobin and the enzymatic activity of creatine phosphokinase CK), MB-isoenzyme of CK (CK-MB), aspartate aminotransferase (GOT), alanine aminotransferase (GPT) and lactic acid dehydrogenase (LDH) were serially determined in 10 patients with acute myocardial infarction. Additionally the same parameters were assessed in 5 patients with angina pectoris for 24 hours after bicycle ergometry. 10 in-patients served as controls. Myoglobin was determined by radioimmunoassay and the other enzyme activities according to the current kinetic methods. Comparison of myoglobin with the enzymatic parameters showed that the myoglobin peak occurs 5.6 hours after the beginning of the sampling period, i.e. 7.3 hours earlier than CK and CK-MB and 11.6 hours earlier than GOT. In analogy to this finding the descending limb of the myoglobin curve was significantly earlier at a level of one third of the peak value, i.e. 8.2 hours earlier than CK-MB, 18.8 hours earlier than CK and 27.3 hours earlier than GOT. No signs of myocardial necrosis in terms of myoglobin or enzymatic activity could be detected after bicycle ergometry. It is concluded that myoglobin is a more sensitive parameter for assessment of the acute phase in patients with myocardial infarction than the usualy enzymatic parameters.

Acute Disease↗
PubMed1979

Comparison of red cell creatine level and reticulocyte count in appraising the severity of hemolytic processes.

In seeking a sensitive indicator for quantitative assessment of hemolytic disease, we found a close dependence of red cell creatine level on cell age. Studies in 21 patients with steady-state hemolysis showed high correlation (r=0.89, p less than 0.001) between reticulocyte counts and red cell creatine levels. Excluding elevation of the creatine level as a variable epiphenomenon of increased erythropoietic activity, density separation of normal red cells revealed distinctly higher creatine levels in younger cells. The reticulocyte counts and creatine levels as quantitative predictors of hemolytic processes were compared: in severe hemolytic anemias (T50Cr less than 11 days), erythrocyte survival (T50Cr) correlated well with creatine levels (r = -0.86, p less than 0.01) and, to a lesser degree, with reticulocyte counts (r = -0.72, p less than 0.05). In milder disease (T50Cr greater than 11 days), however, no correlation existed between reticulocyte counts and T50Cr, whereas the creating levels correlated closely with T50Cr (r = -0.84, p less than 0.001). Thus, on the basis of our regression equations, useful estimation of red cell survival may be obtained from single measurements of erythrocyte creatine.

Adolescent↗
PubMed1978

[Linearity and blank value in the serum creatine phophokinase MB-fraction determination by means of the inhibition test].

The inhibition test shows elevated creatine kinase MB (CK-MB) activity if the test volume is reduced. Hence, sera with considerably increased serum protein values may produce negative CK-MB results. What is involved here is an apparent blank phenomenon dependent on the serum proteins. This effect is not derived from the Inh-CK-M antibody but is directly connected with the CK-reagents, since analogous relationships are also shown with the conventional CK-method. In order to produce reliable inhibition test results, a reflectant blank versus test-volume and serum protein level must be taken into account.

Clinical Enzyme Tests↗
PubMed1975

[Enzymic determination of total serum cholesterol with centrifugal analyzers (author's transl)].

The enzymic determination of total cholesterol described by P. Röschlau (Z. Klin. Chem. Klin. Biochem. 12, 403-407 (1974)) was modified and adapted to the Centrifichem system. The method which requires only 5 mul serum, is accurate, quick and simple. A separate serum blank is not necessary. Compared with the manual method, smaller quantities of reagents used and the resulting decrease in cost are appreciable for large series of analyses. The course of the reaction at 30 and 37 degrees C, the linearity, calibration, precision, recovery, and correlation with the Lieberman-Burchard method and the enzymic manual method are described.

Autoanalysis↗