Intracellular distribution, purification, and properties of dihydrofolate synthetase from pea seedlings.
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Chromatography, DEAE-Cellulose↗
Biomedical subjects
M Kobashi
Publications and source records attributed to M Kobashi.
Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts.
2-Amino-4-hydroxypteridine compounds were isolated as the Crithidia factor from spinach chloroplasts by DEAE-Sephadex A-50, DEAE-cellulose, Sephadex G-25, and thin-layer chromatography. One of the compounds was characterized as 6-hydroxymethylpterin by gas-liquid chromatography-mass spectrometry and by comparison with authentic specimen.
Chloroplasts↗
Quantitative microanalysis of capsaicin, dihydrocapsaicin and nordihyrocapsaicin using mass fragmentography.
A mass fragmentographic method for the quantitative microanalysis of capsaicin, dihydrocapsaicin and nordihydrocapsaicin in the fruits of Capsicum annuum has been developed. The molecular ions at m/e 377, 379 and 365 in the mass spectra were used for monitoring the trimethylsilyl derivatives of capsaicin, dihydrocapsaicin and nordihydrocapsaicin, respectively. The ratios of the height of each molecular ion to that of an internal standard (cholestane) were linear over the range 5-60 ng. The purification of individual capsaicinoids by high-performance liquid, thin-layer and gas-liquid chromatography is also described.
Capsaicin↗
Occurrence of Crithidia factors and folic acid in various bacteria.
Crithidia factors and folic acid were found to be widely distributed in culture fluids and in cells of 27 species of bacteria, when cultured under aerobic conditions into the stationary phase. Most bacteria excreted more Crithidia factors and folic acid than they retained in their cells. One Crithidia factor produced by Serratia indica and one produced by Bacillus cereus differed from biopterin in their chromatographic behavior. The factor excreted by S. indica appeared to be a 2-amino-4-hydroxy-6-substituted pteridine on the basis of KMnO(4) oxidation and ultraviolet absorption spectra. One of the folate compounds excreted by this organism was shown to be identical to 5,10-methylidynetetrahydrofolic acid by bioautography.
Bacteria↗