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Biomedical subjects

M Koide

Publications and source records attributed to M Koide.

At least 181 records · Page 10Linked to original sources
PubMed1989

[Inhibitory effect of a new proglumide derivative, loxiglumide, on CCK action in isolated rat pancreatic acini].

The effect of a new proglumide derivative, loxiglumide (DL-4-(3,4-dichloro-benzoyl-amino)-5-(N-3-methoxy-propyl-pentylamino+ ++)-5-oxo-pentanic acid; CR 1505), on binding of 125I-CCK-8 and amylase release stimulated by CCK-8 was investigated in isolated rat pancreatic acini. Loxiglumide inhibited CCK-8-stimulated amylase release and binding of 125I-CCK-8 to rat pancreatic acini in a dose-dependent manner. Loxiglumide caused a concentration-dependent rightward shift of the dose-response curve for CCK-8-stimulated amylase release without altering the maximal response. Schild plots showed a slope of 0.82 and pA2 value of 7.05. The inhibitory effect of loxiglumide on amylase release was reversible. Loxiglumide significantly inhibited amylase release in response to CCK-8, caerulein and gastrin-I. However, loxiglumide had no effect on amylase release stimulated by other receptor secretagogues (bombesin, carbamylcholine, secretion and vasoactive intestinal polypeptide) or by agents bypassing receptors (A23187 and TPA). These results indicate that loxiglumide acts as a potent, competitive and specific CCK antagonist on the pancreatic acini.

Amylases↗
PubMed1989

Bioassay of plasma cholecystokinin in rat and human: inhibition of protein synthesis prevents the decrease in the sensitivity and responsiveness of isolated rat pancreatic acini to CCK-8.

Isolated rat pancreatic acini were most sensitive and responsive when stimulated directly with cholecystokinin octapeptide (CCK-8) without preincubation. Both the responsiveness and sensitivity of acini to CCK-8 decreased time dependently with prolonged preincubation. When acini were stimulated with CCK-8 following pulse labeling with radioactive leucine, old protein was discharged together with newly synthesized (labeled) protein. However, the sensitivity and responsiveness of these acini for release of labeled protein were primarily reduced with preincubation, indicating that newly synthesized protein was contributing to the time-dependent loss of sensitivity and responsiveness. Then isolated acini were treated with 300 microM cycloheximide for 2 h. This treatment prevented the decreases in the sensitivity and responsiveness of amylase release to CCK-8 stimulation and made these alterations in one series of experiments negligible. Using these acini, a sensitive and specific bioassay for the measurement of CCK in human and rat plasma was developed.

Amylases↗
PubMed1989

[Plasma cholecystokinin concentration in patients with chronic pancreatitis measured by bioassay].

We developed a specific and sensitive bioassay for measuring plasma cholecystokinin (CCK) in human and investigated CCK response after a test meal in patients with chronic pancreatitis. Treatment with cycloheximide increased the sensitivity and responsiveness of isolated rat pancreatic acini to CCK-octapeptide (CCK-8) and thus plasma levels of CCK-8 as low was 0.17 pM were detectable. Fasting plasma CCK levels in normal subjects as CCK-8 equivalents were 0.75 +/- 0.25 pM and rose to a peak of 6.2 +/- 0.68 pM at 45 min after a test meal consisting of 400 ml milk and 2 boiled eggs. Basal and stimulated plasma levels of CCK-8 in patients with non-calcified chronic pancreatitis were significantly higher than those in normal subjects. In contrast, postprandial responses of plasma CCK-8 in patients with calcified chronic pancreatitis was significantly low compared to those in control subjects, although basal plasma levels were not significantly different from those in controls.

Amylases↗
PubMed1989

[A case of congenital absence of the right coronary aortic cusp].

A 12-year-old boy with absence of the right coronary cusp underwent aortic valve replacement with a 21 mm St. Jude Medical prosthetic valve. He was known to have noncyanotic congenital heart disease at the age of 2 months. The diagnosis of aortic insufficiency was first made when he was 1 1/4 years old. Intensive medical treatment was continued while he was growing up. Cardiac catheterization was performed repeatedly at ages 3 1/2 and 7. The findings on the fourth cardiac catheterization at 12 years of age were pulmonary arterial pressure of 90/60 mmHg, left ventricular end-diastolic pressure of 25 mmHg and poor contraction of the left ventricle. Although IABP support was necessary for treatment of severe low cardiac output state in the early postoperative period, he was discharged at 1 month after operation, Now, he goes to school under anticoagulant therapy. Intensive medical care followed by elective aortic valve replacement may be a useful treatment for isolated congenital absence of the aortic valve cusp.

Aortic Valve↗
PubMed1989

[Assessment of cardiac rejection following heart and lung transplantation (I)].

To diagnose the cardiac rejection after the heart and lung transplantation, the histopathological assessment is the most reliable method at the present time. In this study, the heterotopic heart and lung transplantation was performed using rat model and the rejection process of heart allograft was sequentially examined at 1 day, 4 days, 7 days, and 12 days after the transplantation. The gross finding of both ventricles were observed to investigate the development process of the rejection. Then, the most typical findings in each period were analysed using a microscope with our criteria including three components, which are 1. Perivascular findings 2. Interstitial findings, 3. Myocyte degeneration. This study demonstrates that allograft rejection after heart and lung transplantation initiates from right ventricular free wall and spreads into outer layer of left ventricle as same fashion as orthotopic cardiac transplantation. The assessment of these 3 components using the indicators shown in this study might be very useful method to objectively determine the grades of rejection after the heart and lung transplantation.

Animals↗
PubMed1989

[Assessment of cardiac rejection following heart and lung transplantation (II)].

To investigate the histologic consequence of combined heart and lung transplantation, 15 transplanted hearts were obtained from heterotopic heart and lung transplantation using rat model. The allografts were randomly obtained between 1 and 13 days after the transplantation without use of any immunosuppressants. Based upon our criteria established in our previous study, the degree of cardiac rejection was evaluated using rejection score, independently at seven segments in both ventricles, which are 1) Right Ventricle Free Wall (RVFW), 2) Transient zone between RVFW and Septum (RVFW-SEP), 3) Septum (RVSEP), 4) Left Ventricle (Right outer layer) (LVRO), 5) Left Ventricle (Left outer layer) (LVLO), 6) Left Ventricle (Right inner layer) (LVRI), 7) Left Ventricle (Left inner layer)(LVLI). The degree of cardiac rejection in each segment shows differently and the rejection initiates from RVFW and spreads into RVFW-SEP and outer layers of left ventricle. The segments of RVSEP and inner layers of left ventricle remained normal longer than other segments, and were thought to be less sensitive to diagnose the rejection in early phase. Significant correlation between post-transplanted days and the degree of rejection was observed in all segments except RVSEP. In conclusion, this study demonstrates that the myocardial biopsy obtained from RVFW, RUVF-SEP and LVRO after the combined heart and lung transplantation might be more sensitive than any other segments including RVSEP.

Animals↗
PubMed1989

[Single left coronary artery with a fistula to the right ventricle: report of a case with successful closure].

A 4-year-old boy of single left coronary artery associated with a fistula to the right ventricle was reported. Cardiac catheterization and selective coronary angiography revealed single left coronary artery with a fistula to the right ventricle. At operation, a single coronary artery arising from the left aortic sinus was present. A dilated and tortuous left circumflex branch passed to the right and anterior . This vessel terminated into the outflow tract of the right ventricle. The dilated vessel was doubly ligated at just distal portion of a acute marginal branch. The fistula to the right ventricle was directly sutured from arteriotomy. Then three small branches from the anterior descending artery terminating into the terminal portion of the dilated vessel were found and these were ligated respectively. After repair of arteriotomy, the remainder of the dilated and tortuous vessel was wrapped with a Dacron mesh for reinforcement. Postoperative course was uneventful and there were no complications.

Arteries↗
PubMed1989

[Surgical experience with a case of subaortic stenosis previously treated by patch closure of ventricular septal defect].

A patient who developed subaortic stenosis ten years after patch closure of perimembranous VSD was surgically treated. The subaortic obstruction could not relief sufficiently by the conventional transaortic maneuver. In this surgery, additional ventriculo-septoplasty (modified Konno's operation or Cooley's operation) was performed, preserving the patient's own aortic valve, because of the valve and the valvular ring diameter were almost normal. The postoperative course was uneventful. In this case, a posterior deviation of the infundibular septum and the ventricular septal patch seemed to have played an important role in the pathogenesis of the development of subaortic stenosis.

Adolescent↗
PubMed1989

[A case of successful operation of acute pulmonary embolism due to right ventricular thrombus detected by two-dimensional echocardiography].

A 44-year-old woman suddenly developed dyspnea and loss of consciousness four days after cholecystectomy. Under the diagnosis of acute pulmonary embolism, the patient was treated with anticoagulant with temporary relief of her symptoms. But emergency embolectomy was performed because of large right ventricular thrombus detected by two-dimensional echocardiography. Many reports suggest a relationship between pulmonary embolism and right-sided cardiac thrombi. We consider that two-dimensional echocardiography is essential in case of acute pulmonary embolism.

Acute Disease↗
PubMed1989

[Early recognition of exercise-induced myocardial ischemia by plasma free fatty acid].

To investigate whether or not myocardial free fatty acid (FFA) uptake is useful for early recognition of exercise-induced myocardial ischemia compared with myocardial lactate uptake, we studied in 8 patients with stable effort angina pectoris by measuring hemodynamic and metabolic parameters during supine multistage bicycle ergometer exercise (BEx). Analysis of FFA composition was performed by high performance liquid chromatography. The exercise endpoint in the control study was moderate chest pain in all cases. In all cases, BEx testing using the same method, duration and workload was repeated 1.5 hours after the oral administration of 6 mg of nilvadipine (a new calcium antagonist). Exercise time of the subjects studied was 463 +/- 40 sec (mean +/- SE, 180-540 sec). After administration of nilvadipine, chest pain was not induced in 6 patients and was lessened in severity in 2 patients. Mean ST segment depression was 0.21 +/- 0.05 mV in the control period and it was significantly reduced to 0.08 +/- 0.03 mV after nilvadipine administration (p less than 0.05). Myocardial FFA uptake decreased from 21.1 +/- 4.4% at rest to 8.1 +/- 2.1% at peak exercise in the control study (p less than 0.05). Myocardial lactate uptake was unchanged at rest and peak exercise in the control study (21.6 +/- 2.7 vs 22.2 +/- 3.3%). After administration of nilvadipine, myocardial FFA uptake did not change during BEx (25.3 +/- 2.7 at rest and 21.3 +/- 3.6% at peak exercise). At peak exercise, myocardial FFA uptake increased significantly after administration of nilvadipine (21.3 +/- 3.6 vs 8.1 +/- 2.1%, p less than 0.05). In FFA composition, the percentage of each of 12 FFA did not change during myocardial passage at rest and peak exercise.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Atrial septal defect with tricuspid valve regurgitation and heart failure in early childhood: report of a case with successful surgical management consisting of special patch closure technique and use of polydioxanone suture for the tricuspid annuloplasty].

A management of surgery for infant having a small left ventricular cavity associated with atrial septal defect and tricuspid valve regurgitation was presented. A right upper part of the defect was remained in open during a patch closure of ASD and this portion was temporarily sutured by prolene stayed extracardialy through Waterston's groove. During 20 minutes after weaning from the cardiopulmonary bypass, left heart failure did not appear and then it was completely closed. DeVega's method was employed for the tricuspid valve regurgitation using a absorbale Polydioxanone suture, because of a growth of the sutured annulus. Postoperative course was uneventful and trivial tricuspid valve regurgitation was recognized in angiocardiographic studies performed in 2 weeks and 6 months after operation.

Child, Preschool↗
PubMed1988

[Serial copulatory behavior of male hamsters and rats].

The copulatory behavior of mammalian males is generally characterized by the male's repeated approaches to and mounting of the female. The mounting behavior can lead to intromission, and after several intromissions, an ejaculation occurs. Following ejaculation, the male refrains from sexual activity for a period of time, the so-called "post-ejaculatory interval (PEI)". Most mammals will return to copulate again. Both male hamsters and rats were used and each animal performed five series of copulations with a proestrous female. From the 1st to 5th series of copulations the hamsters showed a shorter PEI than the rats. In addition, the PEI of the hamsters showed no change after each ejaculation, while the rats gradually showed a significantly increased PEI during the five series of copulations.

Animals↗
PubMed1987

Polyacrylamide gel electrophoresis of several proteins in the presence of sodium oligooxyethylene dodecyl ether sulfates or a commercially available analog.

The behavior of water-soluble proteins and a typical membrane protein in polyacrylamide gel electrophoresis was studied in the presence of sodium oligooxyethylene dodecyl ether sulfates with a defined number of oxyethylene units or a commercially available analog with distribution and heterogeneity for the oxyethylene chain length and alkyl group, respectively. It was concluded that most water-soluble proteins do not interact with the anionic surfactants as long as their oxyethylene chain lengths are sufficiently long; the commercially available surfactant binds exceptionally well to beta-lactoglobulin without causing denaturation and subsequent dissociation; such surfactants are expected to solubilize membrane proteins without causing denaturation as judged from the result with Na+,K+-ATPase and are promising as new solubilizing agents for membrane proteins which enable efficient electrophoretic analysis or separation after the solubilization.

Animals↗
PubMed1987

Effects of a new second generation calcium channel blocker, nilvadipine (FR34235), on exercise-induced hemodynamic changes in stable angina pectoris.

The mechanism of the antianginal actions of nilvadipine was investigated in 11 patients with effort angina pectoris. Hemodynamic data were obtained by angina-limited supine multistage bicycle ergometer exercise testing before and after a single 6 mg dose of nilvadipine. Compared with chest pain during control exercise testing, pain at peak exercise disappeared or abated and the ST segment at peak exercise also showed less significant depression after administration of nilvadipine. At rest and at peak exercise, mean blood pressure, pulmonary artery wedge pressure and systemic vascular resistance decreased significantly, whereas heart rate and cardiac index increased significantly after nilvadipine. Rate-pressure product and stroke volume index did not change significantly. Coronary sinus flow at peak exercise increased significantly and total coronary vascular resistance at rest and at peak exercise decreased significantly after nilvadipine. The plasma concentrations of nilvadipine 1.5 hours after administration ranged from 1.15 to 8.23 ng/ml. These data suggest that the principal factors in the antianginal actions of nilvadipine are an increase in myocardial oxygen supply due to increased coronary blood flow and a reduction in myocardial oxygen demand mainly by a decrease in afterload and additionally by a decrease in preload.

Adult↗