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Biomedical subjects

M Kokot

Publications and source records attributed to M Kokot.

28 records · Page 2Linked to original sources
PubMed1992

[Estimation of renal tubular function after uropoline administration in certain disease states].

24 h urinary excretion of beta-microglobulin (beta 2M) and Tamm-Horsfall protein (THP) before and after administration 40 ml of 75% Uropoline were assessed, as a specific markers of proximal and distal tubular dysfunction respectively. 22 patients without renal diseases and hypertension (C), 22 hypertensive patients (HP), 14 patients with renal stone disease (RSD) and 16 patients with pyelonephritis (PN) were examined. Administration of Uropoline did not change beta 2M and THP urinary excretion in C, but increased beta 2M excretion in HP and decreased THP urinary excretion in patients with RSD. It is concluded, that Uropoline shows a noxious effect on the proximal tubule in HP and on the distal tubule in patients with RSD.

Adult↗
PubMed1992

[Effects of furosemide, propranolol and nifedipine on urinary excretion of Tamm-Horsfall protein in patients with arterial hypertension].

UNLABELLED: In 65 hypertensive patients the influence of 10 day treatment with furosemide (24 subjects), nifedipine (21 subjects) and propranolol (20 subjects) respectively on urinary Tamm-Horsfall protein (U-THP), sodium and potassium excretion, 24 hour urinary volume and blood pressure was determined. In 23 control subjects the above mentioned parameters were assessed only under basal conditions. In hypertensive patients urinary THP excretion was not different from controls. In all examined groups a significant positive correlation was found between urine volume and urinary THP excretion under basal conditions. Such a correlation was absent after nifedipine or propranolol therapy respectively, but still existed after furosemide administration. No correlation was found between urinary THP and Na and K excretion respectively. In contrast to nifedipine and propranolol, a 10 day treatment with furosemide caused a significant increase in U-THP. CONCLUSIONS: Urinary THP excretion in hypertensive patients did not differ from U-THP in healthy subjects. In contrast to propranolol and nifedipine, treatment with furosemide caused an increase in THP urinary excretion.

Adult↗
PubMed1992

[Urinary excretion of Tamm-Horsfall protein by patients with acute renal failure].

Urinary excretion of Tamm-Horsfall protein (THP), sodium and potassium was assessed in 12 patients 5, 8, 11, 14, 17, and 22 days after the onset of acute renal failure (ARF) and in 23 control subjects. In patients with ARF at the oliguric phase urinary excretion was significantly reduced but significantly increased (when compared with controls) at the onset of the polyuric phase. In contrast to healthy subjects in patients with ARF no significant correlation was found between urine volume and urinary excretion of THP. Normalization of THP excretion was noticed prior to normalization of serum creatinine level. Results obtained in this study prove absence of the physiological relationship between urinary excretion of THP and urine volume in patients with ARF.

Acute Kidney Injury↗
PubMedDate not supplied

Quantification of human ooplasmic mitochondria.

It is likely that there is an association between the fitness of mitochondria and their ability to support normal cellular function. Oocytes are greatly enriched in the number of mitochondria as they support essential developmental processes such as oocyte maturation and embryonic development, while their replication is deferred until gastrulation. The mitochondrial DNA (mtDNA) copy number in 87 human oocytes from 29 patients was evaluated after DNA extraction and real-time quantitative polymerase chain reaction (PCR). The average mtDNA copy number was 795,000 (+/- 243,000) in metaphase II oocytes. mtDNA content varied considerably between oocytes, even within the same patient. No relationship was found between mtDNA copy number and maternal age. The findings suggest that mtDNA replication is fully accomplished by the germinal vesicle stage in the fully developed oocyte.

DNA, Mitochondrial↗