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Biomedical subjects

M Kotschy

Publications and source records attributed to M Kotschy.

104 records · Page 6Linked to original sources
PubMedDate not supplied

Von Willebrand factor in subretinal fluid.

PURPOSE: To measure the vWF antigen concentration (vWF Ag) in subretinal fluid (SRF) and blood plasma. METHODS: Prospective study of 30 patients, aged from 15 to 78 years (mean 52.7 years), undergoing retinal detachment surgery. Excluded were patients with venous or arterial disease or any other factors that could affect the parameters evaluated. Subretinal samples were obtained at the time of routine drainage during retinal detachment surgery. Venous blood samples were taken from the cubital vein into sodium citrate solution (9:1) immediately after induction of anesthesia but before surgery. VWF in plasma and in SRF and its relation to patients' age, sex, the duration and extent of retinal detachment, number of retinal tears, and cryopexy application were evaluated. RESULTS: The median level of vWFAg in 30 samples of SRF was 6.3%. The median level of vWF Ag in blood plasma was 70.34%. The levels of vWF Ag in SRF were significantly lower than in blood plasma (p<0.00001). We found no correlation between the vWF Ag concentration in plasma or in SRF and sex, the degree of myopia, the duration and extent of retinal detachment, number of retinal tears and the use of cryopexy. CONCLUSIONS: Determination of vWF Ag showed that this factor in SRF is unrelated to patients', sex, the degree of myopia, the duration and extent of retinal detachment, number of retinal tears and use of cryopexy.

Adolescent↗
PubMedDate not supplied

Fibrinolytic activity of subretinal fluid after cryopexy.

PURPOSE: To measure the tissue plasminogen activator antigen (t-PA Ag) content and plasminogen activator inhibitor type 1 (PAI-1) activity of SRF and blood. METHODS: 22 patients aged from 20 to 77 years (median: 57.3 years), were studied, undergoing retinal detachment surgery. Excluded were patients with vein or arterial disease and any other factors that could change the parameters evaluated. Subretinal samples were obtained at the time of routine drainage during retinal detachment surgery, after cryopexy. Venous blood samples were taken from the cubital vein into sodium citrate solution (9:1) immediately after induction of anesthesia but before surgery. T-PA Ag concentration and PAI-1 activity in subretinal fluid and citrated plasma and their relation to patients' age and sex, the duration and extent of retinal detachment, and degenerative changes of the retina were assessed. RESULTS: The median t-PA Ag concentration in 22 samples of SRF was 6.7 ng/ml (interquartile range 3.6 ng/ml) and PAI-1 activity 14.0 IU/ml (interquartile range 7.5 IU/ml). The median levels of t-PA Ag and PAI in plasma were respectively 10.7 ng/ml (interquartile range 8.6 ng/ml) and 15.7 IU/ml (interquartile range 12.2 IU/ml). There were no differences between the t-PA Ag concentration and PAI-1 activity in SRF and blood. We found no correlation between the levels of t-PA Ag and PAI-1 activity in SRF and age, sex, the degree of myopia, the duration and extent of retinal detachment, or retinal degenerative changes. CONCLUSIONS: The parameters of the fibrinolytic cascade studied here indicated the presence of high levels of t-PA Ag and PAI-1 activity in SRF.

Adult↗
PubMedDate not supplied

[Blood coagulation activation in patients with ulcerative colitis].

UNLABELLED: Hemostasis disturbances in patients with ulcerative colitis (uc) can result from quantity and quality changes in blood coagulation factors synthesis (acute phase reaction) as well as their consumption in inflammatory processes affecting in large bowel. The aim of this study was to estimate a coagulation system activation in patients with uc. PATIENTS AND METHODS: 42 patients with uc, 21 in active and 21 in inactive disease phase as well as 26 healthy persons were studied. In all concentration of thrombin-antithrombin III complexes (TAT), antithrombin III and prekallikrein activities, von Willebrand factor (vWf) and fibrinogen concentrations, kaolin-kephalin time (aPTT), prothrombin level and platelets count were determined. RESULTS: In patients with uc higher level of TAT, fibrinogen and platelets count, lower level of prekallikrein activity and shorter aPTT were found than in control group. No significant differences in obtained results of hemostatic parameters between patients with active and inactive uc were found. Whereas, it was disclosed lower platelets count and fibrinogen concentration in cases with inflammatory process only to rectum limited in comparison with more extended inflammatory changes. Using multiple regression method it was found that in studied group the TAT concentration, marker of thrombin in vivo generation, was determined by: aPTT, fibrinogen level, disease severity, inflammatory process extension in large bowel, systemic acute phase reaction. CONCLUSIONS: 1) In patients with uc independently of activity and extension of inflammatory process, the increase of in vivo throminogenesis expressed by higher TAT level was found. 2) Observed coagulation system disturbances may exert an influence on inflammatory process affecting large bowel and increase the risk of thrombotic complications in the course of uc.

Acute-Phase Reaction↗
PubMedDate not supplied

Thrombomodulin--endothelial thrombin receptor in blood of patients with unstable angina pectoris.

BACKGROUND: Thrombomodulin (TM) a membrane receptor for thrombin generated in blood in vivo, is present on the surface of vascular endothelium cells. The aim of our work was the determination of thrombomodulin in the blood of patients with unstable angina pectoris. MATERIAL AND METHODS: In the study took part 87 patients with unstable angina pectoris (40 women and 47 men) at age 41-79 years. Thrombomodulin was determined in citrated blood plasma with the use of enzyme immunoassay ELISA with the diagnostic kit manufactured by American Diagnostica. RESULTS: Statistically significant higher thrombomodulin concentration (sTM) was found in the patients when compared with the values recorded in control group. Elevated sTM levels depended on patients age and they were significantly higher in patients over 50 years old. There were no statistically significant differences with respect to patients gender and coexistent risk factors such as arterial hypertension, diabetes or smoking, but significantly higher sTM concentration was observed in patients with high serum cholesterol level. CONCLUSION: Elevated sTM concentrations result from the damage to vascular endothelium cells by the atheromatous process manifested in unstable angina pectoris.

Adult↗
PubMedDate not supplied

Effects of retinal detachment surgery on protein C antigen level.

BACKGROUND: The purpose of the study was to evaluate perioperative changes in protein C antigen (PC Ag) in patients undergoing retinal detachment surgery under general or local anesthesia. MATERIAL AND METHODS: Prospective study of 39 patients: 27 patients, 14 male, 13 female, age from 15 to 78 (mean: 50.8, SD=18.6), undergoing retinal detachment surgery (encirclement with scleral buckling) performed under general anesthesia (group A), and 12 patients, 5 male, 7 female, age from 25 to 78 (mean: 52.9, SD=19.2), operated under local anesthesia (group B). Patients with venous or arterial disease or other factors which could affect the evaluated parameters were excluded from the study. Blood samples were collected from the cubital vein one day before surgery, immediately after induction of anesthesia but before surgery, immediately after the completion of the operation but before the termination of anesthesia, and after surgery (on the 1st and 4th day). The PC Ag concentration was measured in the citrate plasma of the patients studied, utilizing a commercially available enzyme-linked immunosorbent assay. RESULTS: In both groups, the pattern of changes in perioperative PC antigen was similar. A trend towards moderately lower intraoperative levels of PC Ag could be observed. In both groups, PC Ag concentration increased on the first postoperative day, but a significant change was noted only in general anesthesia group. CONCLUSIONS: Retinal detachment surgery evokes a less than statistically significant decrease in intraoperative PC antigen levels in systemic circulation, and these changes are not affected by the type of anesthesia. An increase in PC Ag was observed on the 1st postoperative day, but this was statistically significant only in the general anesthesia group.

Adult↗
PubMedDate not supplied

[Tissue plasminogen activator, its inhibitor and other parameters of fibrinolysis in blood of patients operated for mild hypertrophy of the prostate].

An increased blood fibrinolytic activity manifested by increased tissue plasminogen activator (t-PA) and decreased tissue plasminogen activator inhibitor (PAI-1) and increased FDP levels are seen in 40 patients with mild hypertrophy of prostate. Surgical treatment increased blood fibrinolytic activity manifested in the increase in t-PA, decrease in PAI-1, shortening of ELT, increase in FDP, and decrease in plasminogen and 2-AP activities. Blood fibrinolytic activity was the highest immediately after surgery with tendency to the gradual normalization. Positive ethanol test and decrease in thrombocyte count indicate and activation of blood clotting system induced by the tissue thrombo-elastins released during surgery. Subclinical DCI with the secondary increased fibrinolysis activation is present in patients with mild hypertrophy of the prostate both prior to and after surgery.

Adult↗
PubMedDate not supplied

[Antithrombin III and alpha-2-antiplasmin activities compared with other hemostasis parameters in uncomplicated diabetes mellitus, type 2].

In 40 patients with diabetes mellitus type II without clinical signs of any organ complications and in the respective control group the following indices of hemostasis were assessed: 1) activity of AI-III, 2) activity of alfa-2-AP, 3) fibrinogen, 4) time of fibrinolysis, 5) platelets count, adhesiveness and spontaneous aggregation, 6) kaolin-cephalin and profil stipven-cephalin plasma times. All these indices were normal in uncomplicated diabetes mellitus with the expectation of platelets activity. Stimulation of platelets activity and increase of corresponding parameters appears in diabetes mellitus type II before any other symptoms of angiopathy.

Adult↗
PubMedDate not supplied

[Blood coagulation and fibrinolysis in blood donors after plasmapheresis].

Authors were interested in blood coagulation proteins and fibrinolysis in blood donors following several plasmaphereses. This interest was related to the occurrence of thrombo-embolic and hemorrhagic complications in these subjects. Blood morphology, serum protein, blood coagulation and fibrinolysis have been examined in 40 healthy blood donors, aged between 19 and 46 years, who gave blood plasma by plasmapheresis technique for 1-59 times. Results did not show any significant changes in blood morphology and serum protein levels prior to and after consecutive plasmaphereses. No significant decrease in blood coagulation proteins and fibrinolysis has been noted. However, a significant increase in factors VIII and IX activities was noted in several blood donors, who underwent the largest number of plasmaphereses. It may predispose these donors to thrombo-embolic complications.

Adult↗
PubMedDate not supplied

[Effect of vascular surgery on values of thrombin-antithrombin III complexes in patients with atherosclerosis of lower limb arteries].

In 25 patients with atherosclerosis of lower limb arteries (ALLA) in whom vascular operation was performed, the level of thrombin-antithrombin III (TAT) complexes and antithrombin III (AT III) activity were determined in comprehensive studies of the blood clotting system. The comparative group for the assessment of operation effect on primarily not disturbed haemostasis in humans included 10 men operated on for inguinal hernia, while the control group consisted of 54 healthy people. Blood for the determinations was taken before the operation, on the day of the operation (0-30 minutes after the operation), on the first day after it, and also on the third day in patients with hernia, and on the 7th day in patients with ALLA. In the patients with ALLA, blood hypercoagulation features were found, expressed as increased level of TAT complexes in spite of lack of abnormalities in comprehensive coagulation studies, while in the patients with hernia only increased AT III activity was found. The surgical operation caused in the patients with ALLA enhancing of hypercoagulation which was evidenced by over threefold increase of TAT complexes on the day of the operation.

Adult↗
PubMedDate not supplied

[Tissue plasminogen activator (T-PA) and tissue plasminogen activator inhibitor (PAI-1) in patients after head injury].

The following factors of fibrinolysis: tissue plasminogen activator (t-PA) and tissue plasminogen activator inhibitor-1 (PAI-1) play an important role in patients after trauma. Their possible mechanisms in head-injured patients remain unknown. We studied the maintenance of those markers of fibrinolysis in the plasma and cerebrospinal fluid of 19 patients after severe head injury (initially GCS less than 8 p) without intracranial haematoma. We measured changes of the level of t-PA antigen and PAI-1 activity in days 0-3, 4-6 and later. T-PA antigen level in the plasma was higher than normally (4-8 ng/ml). T-Pa was present in the cerebrospinal fluid, but its level reached only 30% of the plasma level. In the days following injury the t-PA antigen level decreased. The PAI-1 activity in the plasma was normal (0-15 IU/ml). However, its activity in csf was high and reached, 80% of the plasma level and systematically increase in the following days particularly in patients who died. PAI-1 activity can be connected with the presence of damaged brain tissue and its necrosis and its increase can be a marker of poor prognosis.

Adult↗